Noise affects the accuracy of three-dimensional shape recovery. Its occurrence is unpredictable and depends on several mechanical, environmental, and other factors. When two-dimensional image sequences are obtained for shape from focus (SFF), mechanical vibration occurs in the translational stage, causing an error in the three-dimensional shape recovery. To address this issue, mechanical vibration is modeled using Newton's second law and the principle of the rack and pinion gear. Then, an optimal sampling step size considering the mechanical vibration is suggested through theoretical demonstration. Experiments conducted with real objects verify the effectiveness of the proposed sampling step size. In this paper, in a realistic environment with noise, the potential of obtaining more accurate three-dimensional reconstruction results of the objects is explored by acquiring the optimal sampling step size, which improves the sampling step size relative to those reported in a previous study performed under similar conditions.