In order for electro-optical tracking system (EOTS) to have accurate target coordinate, accurate navigation results are required. If an integrated navigation system is configured using an inertial measurement unit (IMU) of EOTS and the vehicle's navigation results, navigation results with high rate can be obtained. Due to the time-delay of the navigation results of the vehicle in the EOTS and scale factor errors of the EOTS IMU in high-speed and high dynamic operation of the vehicle, it is much more difficult to have accurate navigation results. In this paper, an integrated navigation system of EOTS which compensates time-delay and scale factor error is proposed. The proposed integrated navigation system consists of vehicle's navigation system which provides time-delayed navigation results, an EOTS IMU, an inertial navigation system (INS), an augmented Kalman filter and integration Kalman filter. The augmented Kalman filter outputs navigation results, in which the time-delay of the vehicle's navigation results is compensated. The integration Kalman filter estimates position, velocity, attitude error of the EOTS INS and accelerometer bias, accelerometer scale factor error, gyro bias and gyro scale factor error from the difference between the output of the augmented Kalman filter and the navigation result of the EOTS INS. In order to check performance of the proposed integrated navigation system, simulations for output data of a measurement generator and land vehicle experiments were performed. The performance evaluation results show that the proposed integrated navigation system provides more accurate navigation results.