Chitosan is applied as a dressing for oral mucous wound and a tampon following radical treatment of maxillary sinus. Furthermore, it is being investigated as an absorbing membrane for endodontic and periodontic surgeries. A few studies have reported osteoconduction and osteogenesia at the site of chitosan implant in vivo. However, compared with soft tissue healing processes, the mechanisms concerning effects of chitosan for biological mineralization have not yet been resoil In the present study, we studied the gene expression pattern using cDNA microarray and RT-PCR analyses in hard tissue forming osteoblasts cultured with water-soluble and low molecular weight chitooligosaccharide. cDNA microarray analysis revealed that 16 genes were expressed at 〉1.5-fold higher signal ratio levels in the experimental group compared with the control group after 3 days. RT-PCR analysis showed that chitosan oligomer induced an increase in the expression of two genes, CD56 antigen and tissue-type plasminogen activator. Furthermore, the expression of mRNAs for BMP-2 was almost identical in the experimental and control groups after 3 days of culture, but slightly increased after 7 days of culture with chitosan oligomer. These results suggest that a super-low concentration of chitooligosaccharide could modulate the activity of osteoblastic cells through mRNA levels and that the genes concerning cell proliferation and differentiation can be controlled by water-soluble chitosan.

The aim of this study was to compare the effects of three Ni-Ti instruments on leaning ability by evaluating the volumetric and morphological changes in the apical 6mm of the root canals before and after preparation, using three-dimensionally reconstructed root canals of extracted human teeth. Forty-five teeth were used in this study. They were opened the chambers and removed the all pulp remnants ultrasonically. Subsequently, the canal wall was coated with silver paste and prepared using ProTaper, ProFile and GT rotary files according to the manufacturers instructions. Before and after root canal preparation, all the specimens were scanned with micro computed tomography and examined the differences in dentine volume removed, canal straightening, the proportion of the unchanged area and canal transportation. Quantitative analysis revealed that instrumentation increased in canal volume ranging between 0.081 and $1.866{\;}\textrm{mm}^3$. On average, the large apical preparation produced by ProTaper demonstrated smaller proportions of unchanged surface areas compared to the two other instruments in small canals. But in large canals like maxillary central incisor, the preparation of ProTaper instruments was not enough. ProTaper instrument was tended to increase more in canal volume as compared with the other two instruments but unchanged area was no significant difference. These results showed that three instruments had similar preparation ability and micro computed tomography in combination with the coated wall of root canal using silver paste is a nondestructive and valuable tool to study root canal geometry and changes after preparations in detail.

Neurogenic inflammation has been recognized to play an important role in initiating and sustaining of pulp inflammation. The pulpal innervation may modulate several aspects of the inflammatory response via secretion of neuropeptides. In this present study, these neuropeptides that may be questioned about roles in recruiting leukocytes by inducing the release of the chemokine IL-8 in the pulp during inflammation were tested. The response of human pulp cells in releasing IL-8 after the stimulation with SP and/or CGRP were investigated.(omitted)

Pulpal inflammation is a kind of neurogenic inflammation and it shows vascular changes such as vasodilation and changes in vascular leakage. Various kinds of neuropeptides including substance P (SP) are known to be involved in the pulpal inflammation. The purpose of this study was to investigate the influence of NK1 receptor antagonists on the pulpal blood flow (PBF) when applied iontophoretically through the dentinal cavity of the teeth in order to understand whether iontophoretic ally applied NK1 receptor antagonists can control the pulpal inflammation.(omitted)

IL-1${\alpha}$ and TNF-${\alpha}$ play an important role in initiating and coordinating the cellular events that make up the immune response to infection. The purpose of this study was to examine the effects of proinflammatory cytokines on mineralization and HO-1 protein expression in the human pulp cells. Human pulp cell cultures between the fifth and sixth passage were used in this study. Alkaline phosphatase and osteonectin were selected as markers for mineralization that is, odontoblast-like differentiation.(omitted)

The purpose of this study was to quantify the amount of remaining gutta-percha/sealer on the walls of root canals when three types of nickel-titanium rotary instruments and a hand instrument were used to remove these materials. Forty extracted mandibular premolars were prepared by step-back method and obturated with gutta-percha and sealer. Gutta-percha removal for group 1 was done using hand file with chloroform, group 2 using Profile and group 4 using K3. The following factors were evaluated : Time taken to reach working length, total time for gutta-percha removal and number of fractured instruments.(중략)

I. Objectives Endodontic disease is caused primarily by bacteria that interact with periradicular host from the root canal system. Chlorhexidine gluconate is known to effective to eliminate Enterococcus faecalis which resists to other intracanal medicaments. The aim of this in vitro study was to develop a slowly releasing root canal disinfectant using using chlorhexidine gluconate and chitoic acid. II. Materials and Methods Three different group were prepared with different drug release mechanism. In group A, paper points as used core material were loaded with 20% chlorhexidine gluconate.(omitted)

I. Objectives This study was done to evaluate the shaping ability of ProFile and ProTaper, K-Flexofile during instrumentation in simulated resin canals with different angles of curvature. II. Materials and Methods Ninety simulated root canals with a curvature of 15, 30 and 45 degree (following Schneider's method) were made of epoxy resin ($EPOXICURE^{TM}$, BUEHLER, USA) for this study. The canal length of simulated root canals was adjusted to 18 mm. The beginning point of curvature was positioned at 10mm from the canal orifice.(omitted)

The purpose of this prospective study was to assess the incidence of flare-ups among patients who received endodontic treatment for the last 11 months and to examine the correlation with pre-operative and operative variables. Data were gathered on 840 teeth of 755 patients over 11 months. At first visit, information was obtained on each patient as to their demographics, presenting signs, symptoms and diagnosis, and treatment performed. Treatment procedures were routine. If additional appointments were necessary, the access cavity was closed with a dry cotton pellet and ZOE.(omitted)

It is generally agreed that endodontic success often depends on canal debridement, disinfection and canal obturation. Access opening to a canal is one of the key to canal debridement. Advantages of straight line access opening are allowed a greater proportion of the root canal walls to be prepared than conventional lingual access cavities, minimized the loss of the tooth structure, reduced incidence of file fracture in curved canals. The purpose of this study is determine the influence of different access opening methods on fracture strength in endodontically treated teeth.(omitted)

I. Objectives The purpose of this study was to evaluate the ratio of gutta-percha area in the canal after canal obturation with Continuous Wave of Condensation Technique with varying depths of plugger penetration. II. Materials and Methods Forty extracted human teeth with single canal were divided into four group of ten teeth each. Root canals were prepared up to size 40 of 0.06 taper with $ProFile^{\circledR}$. After drying, canals of three groups were filled with Continuous Wave of Condensation Technique with System $B^{TM}$ and different plugger penetration depths of 3, 5, and 7mm from the apex.(omitted)

Heme oxygenase(HO) is a microsomal enzyme, widely distributed in mammalian tissues, which has a major role in heme metabolism. The role of HO in different tissues has not, as yet, been fully characterized, but it is becoming evident that it is involved in a variety of cellular regulatory and protective mechanism. Therefore, in this report, we confirmed the idea of whether the presence of HO in human pulpal cell, and HO can be a principal mechanism of nitric oxide(NO) mediated pulpal cell damage, by adding a deprivation of NO and to gain clinical relationship. We also accessed the effects of HO in pulpal cells treated with hydrogen.(omitted)

I. Objectives Intracanal medicament with antibacterial action is required to maximize the disinfection of the root canal system. The purpose of this study was to evaluate the efficacy of 2% chlorhexidine digluconate(CHX) and controlled release device(CRD) containing 20% CHX and chitosan coating, compared to calcium hydroxide as intracanal medicament against E. faecalis. II. Materials and Methods One hundred and twenty intact freshly extracted bovine incisors were used and were 0.5% NaOCl. Middle 1/3 portion of roots were sliced into 4mm thick section and cementum was removed using diamond burs and external diameter was approximally 6mm.(omitted)

pH varies in the oral environment and on tooth surfaces. The surface of a plaque covered resinous restoration has a low pH. In human dental plaque a number of acids are produced by bacterial metabolism. The predominant acids are acetic, propionic and lactic acids. These acids induced a surface swelling of restorative resin. The swelling cause formation of pores inside the resin matrix from which organic substances can be released, resulting in a mass loss. The purpose of this study was to qualitatively and quantitatively analyse the leached monomers of dental composites after storage in acetate buffer solution as a function of time by means of high performance liquid chromatography(HPLC) / mass spectrometer and further determine whether pH and time has any influence on the degradation behavior of composite restoration.(omitted)

The purpose of present study was to evaluate the polymerization shrinkage stress and cuspal deflection in maxillary premolars resulting from polymerization shrinkage of composites and compomers. 1)Measurements of polymerization shrinkage stress. For measurements of polymerization shrinkage stress, Stress measuring machine(R＆B, Daejon, Korea) was used. Composites and compomers which were used in this study were as follows：(omitted)

In recent years, the use of Microtomograph in dentistry were proposed and its applications are increasing. The purposes of this study is to evaluate the micro leakage in Class V restorations by using Microtomograph and to compare with other leakage test methods. Using high speed round bur, Class V cavities were prepared to the buccal sides of sixty extracted human upper premolars and randomly distributed to 4 experimental groups and restored as follows.(중략)

The clinical performance of light polymerized composite resins is greatly influenced by the quality of the light curing unit used. Commonly used halogen light curing units(LCUs) have some specific drawbacks such as decreasing light output with time. To overcome this, the blue LED LCUs are newly developed and introduced. The purpose of this study was to observe the reaction kinetics and the degree of polymerization of composite resins when cured by different light sources and to evaluate the effectiveness of the blue LED LCUs compared with conventional halogen LCUs.(omitted)

The purpose of this in vitro study was to measure and compare the in vitro micro shear bond strengths of the following dentin bonding systems to different dentin surfaces under simulated pulpal pressure. Occlusal surfaces of 180 extracted human molars were prepared to expose the dentin surface in perpendicular to axis of tooth. Teeth were randomly assigned to 3 equal groups and subdivided into 4 equal groups. 4 adhesive systems(All Bond 2, SEbond, AdheSE, Prompt L-Pop) were used in this study.(omitted)

The purpose of this study was to evaluate the effect of multiple application of all-in-one dentin adhesive system on microtensile bond strength using confocal laser scanning microscope and microtensile bond strength test. The dentin surface of human molars, sectioned to remove the enamel from the occlusal surface were prepared. In group I, Scotchbond Multipurpose(SM, 3M ESPE) was applied by manufature's recommended. In group II, after the all-in-one adhesive, Adper Prompt L-Pop was applied for 15s, and light cured for 10s, the second coat was re-applied and light-cured.(omitted)

I. Objectives This study investigated the effect of rewetting agent on dentinal microtensile bond strengths(${\mu}TBS$), using one bottle adhesives. II. Materials and Methods Non caries human molars were sectioned to exposed the superficial dentin surfaces, etched 15 seconds using 32% phosphoric acid and 10 seconds rinsed. Samples were randomly divided into two groups according to adhesives (Single Bond, One-Step) used. Each group were subdivided into five groups by different dentin surface treatment：15 second dry(D), blot dry(BD) or 15 second dry and rewetted with distrilled water(DW), Gluma Desensitizer(GD) and Aqua-Prep(AP) during 30 second, respectively.(omitted)

I. Objectives The purpose of this study was to evaluate the effort of a modified luting procedure called "dual bonding technique" by compare micro-shear bond strength to different luting procedures of ceramics to dentin. II. Materials and Methods Eighty-four freshly extracted caries-and restoration-free human third molar were embedded in epoxy resin using acrylic ring. Flat superficial occlusal dentin surfaces were obtained, initially using a Low speed diamond saw and exposed detin surface was group with ＃600-grit SiC paper.(omitted)

This study was done to evaluate the shear bond strength between light-cured glass ionomer cement base and resin cement for luting indirect resin inlay and to observe bonding aspects which is produced at the interface between them by SEM(Scanning Electron Microscope). 1)Shear bond strength. Light-cured glass ionomer cement base were made in plastic molds(10mm diameter, 5mm thickness). Two type of light cured glass-ionomer cement were uesd.(중략)

Composite inlays are indicated for large cavities and different approaches have been proposed to improve the adaptationof Class II restorations, including applying base. The purpose of this study was to compare in vitro the marginal adaptation of class II(MOD) composite inlays(Tescera ATL system, Bisco) made with or without bases, having different physical properties. Extracted human lower molars were used for this study. The base was made from Aeliteflo(Bisco), Dyract AP(Bisco) or Fuji II LC improved(GC) respectively and the comtrol group has no base.(omitted)

There were attempts to reduce the micro leakage and surface roughness of resin composite. One of them is surface sealing. The purpose of this study was to compare the effect of materials, specifically developed for surface sealing, on microleakage and surface roughness in Class V composite restorations. Twenty five standardized Class V cavity preparations were made on the facial surface of extracted human premolars and were randomly assigned to 5 groups. The teeth were restored with Z-250 resin composite after applying Single Bond adhesive system.(중략)

I. Objective This study evaluated the microshear bond strength of teeth bleached with commercial whitening strips and compared with those bleached with home bleaching gel. II. Materials and Methods Twelve exrtacted central incisors were cut into pieces and central four segments were chosen from each tooth and embedded in acrylic resin. Four blocks with 12 tooth segments embedded in acrylic resin were acquired and numbered from one to four. Block 1 was bleached with Crest Whitestrips, block 2 with Claren, block 3 with Opalescence tooth whitening gel(10% carbamide peroxide).(omitted)

I. Objectives Newly all-in-one adhesives were developed for reducing the techique sensitivity and chair time, but lots of concerns were made on bondability, longevity, and microleakage. The object of this study was to measure micro leakage and marginal quality of all-in-one adhesives using electrochemical method and SEM analysis quantitatively. II. Materials and Methods After making Class V cavities, they were bulk filled with Heliomolar(＃A1) after surface treatment with three adhesives：Adper Prompt (Group 1), One up bond F (Group 2), Xeno III(Group 3).(omitted)

The majority of studies comparing the mechanical properties of brittle dental restorative materials now include use of the Weibull Modulus (m). This modulus is determined from analysis of the statistical distribution of strength measurements, which can be determined using a variety of methods, including biaxial flexure, 3pt bend and 4pt bend. In comparing materials it is usually implicitly assumed that the modulus (m) is independent of test method although it is recognised to be highly dependent on flaw distributions. However, in some cases flaw distributions can be modified by sample preparation and test method may modify stressing patterns. This study investigated the pattern of strength and m in two light setting materials.

I. Objectives This study investigated the dentin shear bond strength and the degree of conversion (DC) of currently used dentin bonding agents (DBAs) that were irradiated with a light emitting diode (LED) light curing unit (LCU) and a halogen LCU. II. Materials and methods The halogen LCU and the LED LCU used in this study were a VIP(Bisco, Schaumburg, IL, USA) and an Elipar Freelight(3M ESPE, St Paul, MN, USA) respectively. For the VIP, $400mW{\cdot}cm-2$ intensity mode was used to adjust to the intensity of the LED LCU. The DBAs used in this study were Scotchbond Multipurpose (3M ESPE), Single Bond (3M ESPE), One-step(Bisco), Clearfil SE Bond(Kuraray), and Adper Prompt(3M ESPE).(omitted)

Microcomputed tomography (MCT) made it possible to investigate the morphology of root canals more accurate. The aim of this study was to compare the effects of four root canal shaping methods, the profile system, the ProTaper system, a combination of these, and stainless steel K-files, in the shaping of the mesial root canals of extracted human mandibular first molars, three-dimensionally using MCT. Eight extracted mandibular first molars were scannde using MTC before and after the root cannals were instrumented. Two specimens were prepared using the Profile system and another two specimens were prepared using MCT before and after the root cannals were instrumented. Two specimens were prepared using the Profile system and another two specimens were prepared using the ProTaper system according to manufactures recommendation.(omitted)

I. Objectives The purpose of this study was to investigate, histologically, healing in the periodontal tissue after mechanical furcation perforations using Mineral Trioxide Aggregate (PRO ROOTMTA). II. Materials and Methods These experiments were carried out on mandibular and maxilla premolars and molars obtained from 12 dogs more than one year old and which had clinically healthy periodontia. A total of 34 perforations were made. These were divided into Control(9), MTA(25) groups respectively. A sterile round bur (1mm in diameter) was used to create a mechanical perforation in the furcal floor.(omitted)

I. Objectives The aim of this study was to evaluate and compare the effectiveness of sealer placement in simulated root canal extensions using the K file, ultrasonic file, lentulo spiral and EZ-Fill. II. Materials and Methods Forty resin blocks were attained from cutting Endo-training Bloc with diamond saw. In each parallelepiped block, the simulated root canal was made with ＃20, 08taper GT file. After each block was longitudinally split into two halves using mallet and chisel, a standardized groove of 4mm in length, located 2mmapart from the root apex, was prepared on one wall of two halves using the custom-made knife to simulate the canal extensions with various irregularities.(omitted)

I. Objectives This study investigated the quantity of debris which was extruded apically after canal instrumentation using different types of enlarging instrument in endodontic resin models. II. Materials and Methods Five groups of 9 endodontic resin model were instrumented using each different technique：hand instrumentation without early coronal flaring, hand instrumentation after early coronal flaring, and three nickel-titanium engine-driven instrumentations(Hero 642, Protaper, K3) after early coronal flaring.(omitted)

The purpose of this study was to search non-invasive and reproductive pulp test. Temperature of the crown surface was measured using the infrared thermography, and the pulp test was investigated with difference of crown temperature of the vital and the non-vital tooth in vitro and in vivo. Twenty extracted human maxillary central incisors were used in this study. Two sample teeth after access cavity preparation were arranged setting with one pair. Then, the each tooth wes estimated as the vital and the non-vital tooth.(중략)

The purpose of this study was to apply the photoplethsmography(PPG) as a non-invasive tool to evaluate the blood flow of the anterior tooth in clinic. The system consist of two light-emitting diodes(LED) powered by 5V as the light source. The LED was designed to two weve lengths, 940(infrared) and 660(red)nm simultaneously with a 36nm bandwidth at half the peak intensity. The 54 anterior vital tooth were examined and the measured was analysed in frequency domian (power spectrum).(omitted)

Recent study reported whether the cultured human pulp cells increase IL-8 secretion in response to SP stimulation22). In the present study, whether induction of IL-8 or MCP-1 in pulp tissue can be detected using enzyme-linked immunosorbent assay(ELISA) with ex vivo pulpal explants exposed to neuropeptides in culture and the IL-8 expression using immunohistochemical analysis with the ex vivo pulpal explants exposed to neuropeptides was evaluated. To investigate further mechanisms that may contribute to leukocyte recruitment in lesions of endodontic origin, the differential expression of IL-8 and MCP-1 by human dental pulp tissues stimulated in vitro by the Substance P was examined.(omitted)

The aim of this study was to investigate the success rate of endodontic microsurgery. Three calibrated examiners evaluated a total of 51 teeth that were treated with endodontic microsurgery in Samsung Medical Center, Seoul, Korea, during the period of 1996-2000. The periapical radiographs were evaluated and compared with the preoperative and follow-up radiographs. In addition, a standardized questionable was used to assess the clinical status of each tooth. The data was integrated and each case was categorized as being a clinical success, questionable success or a failure.(omitted)

I. Objectives 17% EDTA is known as an effective solution to remove smear layer. When it's applicated for more than 1 minute, it shows dentinal erosion of intertubular and peritubular dentin. This study is to investigate the effect of dentinal tubule erosion with different time application of 17% EDTA solution on the apical leakage. II. Materials and Methods 35 recently extracted human teeth with single canal, straight root, and closed apex were used in this study. Crowns were removed and the pulp tissue remnants were removed with a barbed broach.(omitted)

In the infected immature tooth with periapical involvement, the pulp is considered to hardly exist in the canal and periapical area. Such a tooth receives apexification procedure, because revascularization of the pulp chamber is in principle not expected. Apexification is beneficial to induce further development of an apex to close the foramina, but does not promote the thickness of the entire canal wall dentin. It may be possible for the pulp to be only partially necrotic and infected when an extremely large communication from the pulp space to the periapical tissues exists with a very young tooth. If this were the case, vital pulp in the apical part of the canal could proliferate new pulp into the coronal pulp space by the successful removal and disinfection of the necrotic infected coronal pulp.(omitted)

I. Objectives The purpose of this study was to evaluate the effects of EDTA on microhardness of roor canal dentine according to different working time. II. Materials and Methods Forty recently extracted, intact single root teeth were used. Debris and soft tissue remnants on the root surface were cleaned with scaler and stored in saline at $4^{\circ}C$ until used. All selected roots were similar in size and shape. 1. Preparation of teeth. The crown of the teeth were removed at the level of the CEJ, using a water-cooled diamond bur.(omitted)

Mesiobuccal or distobuccal canals of maxillary molars and mesiobuccal or mesiolingual canals of mandibular malars were selected. Forty specimens were divided into four groups according to the instruments used : stainless steel K-files($MANI^{\circledR}$, Matsutani Seisakusho Co. Takanezawa, Japan), nickel-titanium K-files (Naviflex $NT^{TM}$, Brassler, Savannah, U.S.A.), $Profile^{\circledR}$, and $ProTaper^{TM}$ (Dentsply-Maillefer, Ballaigues, Switzerland). Occlusal surface of each tooth and root apex were flattened by using diamond burs and sandpaper. Before instrumentation, a rubber stop was fixed with glue to the ＃10 K-files of initial working length in each tooth.(omitted)

Apical periodontitis is inflammation of the periodontium caused by infection of the pulp canal system. Moreover, a dental periradicular lesion occurs as a periradicular tissue reaction to bacterial infection and consists of periradicular inflammation with alveolar bone destruction and root resorption, a consequence of the interaction between oral flora and the existing host defenses. Many investigations dealing with the pathogenesis and history of periradicular lesions have described histologically, immunologically, biochemically the development of the periradicular lesion；but none of these studies have shown any correlation between this lesion and several factors, the whole body disease in the worldwide.(omitted)

I. Objectives The purpose of this study was to compare in vivo the biocompatibility of new calcium phosphate-based root canal sealers(CAPSEAL I, CAPSEAL II) with another type of commercially available calcium phosphate sealer (Apatite Root Sealer type I, Apatite Root Sealer type II) and zinc oxide-eugenol-based sealer (Pulp Canal Sealer EWT) after implantaion in rat subcutaneous tissue. II. Materials and Methods 64 Sprague-Dawley rats were used. There were five groups of three animals each for experimental period of 1, 2, 4, and 12 weeks. The teflon tubes, 5mm in length with an inner diameter of 1.5mm, were washed with ethanol and distilled weter and autoclaved.(omitted)

I. Objectives The purpose of this study is to determine if propagation of pre-existing manufacturing defects results in brittle fracture of NiTi rotary files by examining the fracture surface of the instrument by SEM, and to compare the fracture time of NiTi rotary files with different flute designs under cyclic loading. II. Materials and Methods This study examined three groups of rotary NiTi instruments (K3, ProFile, Hero). Of each group, 04 taper, size 30 and 06 taper, size 25 were selected.：120 in total. Surface defects were created by simulating the machining process of NiTi rotaries.(omitted)

I. Objectives This study was done to evaluate which type of Ni-Ti instrument be able to perform canal shape well in the simulated canal with abrupt curvature near it's apex. II. Materials and Methods A total of 96 simulated root canals were made in epoxy resin(EPOXICURETM, BUEHLER, USA), ＃15 finger spreader (MANI, Japan) were used as root canal templates. The simulated root canal were made with radius of curvature of 1.5 mm, 3.0 mm, 4.0 mm, 6.0 mm respectively, and angle of curvature of all simulate camals was 90 degree.(omitted)

The purpose of this study were to evaluate the effect of epinephrine-containing local anesthetics on pulpal blood flow (PBF) and to compare the change of pulpal blood flow after cavity preparation between in non-anesthetized teeth and in anesthetized ones in cats. Nine cats were initially anesthetized with intra-muscular injection of keramine(75mg/kg) and acepromazine(2.5mg/kg). Periapical radiographs of canine teeth were taken, followed by intra-venous injection of alpha-chloralose(40mg/kg) and urethane (500mg/kg) through the femoral vein for the general anesthesia.(중략)

I. Objectives Endodontic success depends on thoroughly cleaned and completely obturated root canal system. Effective cleaning and obturation will be achieved by well shaped canal. Numerous methodologies evaluating the efficacy and safety of canal preparation has been developed and the use of micro-computed tomography(MCT) in endodontic research is one of the latest innovations. This scientific tools could overcome the inherent limitations of other methodologies, and possesses the ability to visualize morphological characteristics in a detailed and accurate manner without destruction of the tooth and offers reproducible data in all three dimensions. The purpose of this study was to determine the optimal master apical file size with less transportation and more efficiency in removing the infected dentin. For this purpose we evaluated the transportation of canal center and change of untouched area after preparation sequentially from ＃25 file through ＃40 file with 3 different instruments：Stainless steel(SS) K-type hand instruments(MANI, Japan), ProFile.04 instruments (Dentply Tulsa Dental, USA) and Lightspeed instruments(Lightspeed Technology, San Antonio, USA) using micro-computed tomography.(omitted)

Endodontic rotary NiTi(Nickel-Titanium) files have several advantages, but they also have some problems. Fracture of instrument is the one of the problems. Cyclic fatigue fracture may not give a previous sign(ex, bending or distortion of the files) before it happen. It is affected by various factors. This study investigated time which takes for cyclic fatigue fracture to happen and fracture patterns with regard to different NiTi files and pecking motion.(omitted)

I. Objectives Effective endodontic obturation must provide a complete, three-dimensional filling of the root canal system, preventing the communication of fluids between root canal and surrounding periapical tissues. The objective of this study was to compare the apical leakage with or without the use of dentin adhesive system. II. Materials and Methods Forth extracted human teeth with single canals were decoronated. Root canals were instrumented using GT rotary files and Profile .04 file up to #40, 1mm short of the apex. After each instrumentation, copious irrigation with 5.25% sodium hypochlorite solution and confirmation of apical patency were performed.(omitted)

AQ Bond Plus has the function of self-etching priming adhesive, which can be applied by single coating without second coating, being different from conventional AQ bonds. Moreover, because the absorption range of light became wider, the bond can comply with any visible light curing units. Thus, the bond can produce an unified form between the dentine layer with impregnated resin of good quality and the thin and hard film characteristically. In this study, we investigated the junctional conditions of AQ Bond Plus, using a scanning electron microscope (SEM).(omitted)

I. Objectives It is reported that there are complements and immunogloblins in serum from dental pulp in dentinal tubules, and it is thought that dental caries bacteria is opsonized by these serum ingredients, and it is presented by dendritic cells(DCs) in dental pulp. So, we examined whether a maturational difference of DCs occured when S. mutans was opsonized. II. Materials and Methods PBMC was divided from normal human peripheral blood and collected CD14 positive cells by magnetic beads system. Adherent cells were incubated in 5% FCS-RPMI medium included GM-CSF, IL-4 for seven days.(omitted)

I. Objectives The purpose of this study is to evaluate the effect of immediate or delayed composite placement on dentinal micro-tensile bond strength. Caries-free human third molars were used in this study. At first, the occlusal enamel was removed so that the middle dentin was exposed. And then the exposed dentin was polished with 1200-grit silicon carbide paper for the formation of smear layer. The polished dentin was etched with 32% phosphoric acid, dried with gentle air and applicated by dentin adhesives. The specimens were divided into 12 groups.(omitted)

I. Objectives Newly-designed method was evaluated to measure the linear polymerization shrinkage of light-cured resin composites. II. Materials and Methods A resin composite(Clearfil AP-X, Kuraray, Japan) was filled into a vinyl-polysiloxan mold(1 to 8mm-depth, and 7mm-diameter). As indicators, 4 beads(Retention Beads 2 SS, GC, Japan；particle size $200{\;}\mu\textrm{m}$) were placed diagonally on the resin surface of a mold. The coordinates (x, y, z, pasition) of each indicator were measrued by a measuring-microscope after which the resin surface was irradiated by a curing-light source(Optilux 500, Demetron/Kerr, USA) for 40 sec. After 20 sec the coordinates were again measured.(omitted)

Novel silane coupling agents containing hydrophobic phenyl group 3-(3-methoxy-4-methacryloyloxyphenyl) propyl-trimethoxysilane(p-MPS), -triisocyanatesilane (p-MBI), -trichlorosilane (p-MBC) were synthesized. The bonding durability of these silanes against water immersion and thermal stress was investigated. 3-methacryloyloxypropyltrimethoxysilane (3-MPG) was used as a control. The glass modified with those silanes at a concentration of 2wt％ were kept for 3 minutes at $120^{\circ}C$, and then were bonded to the heaped metal with self-cured resin composite.(omitted)

There have been many studies that have evaluated success and failure of endodontic treatment (Nair, Sjogren), but there is remarkably limited information concerning the specific microorganisms that are involved in the teeth with treatment failure. Microorganisms that survive root canal treatment to cause a persistent infection must possess specific characteristics to avoid the host defense. These can be broadly classified as; 1. Sequestration：A physical barrier between the microbe and the host. 2. Cellular evasion：Microorganisms avoid leukocyte dependent antibacterial mechanisms. 3. Humoral evasion：Extracellular bacteria avoid the hosts antibodies and complement.(omitted)

The purpose of this presentation is to investigate whether the certain therapy resistant bacteria can impair the immune defense system in the pariapical tissue. Recent studies have reported that the facultative or obligatory anaerobic bacteria such as Fusobacterium nucleatum, Enterococcus faecalis and Actinomyces species and Gram positive facultative bacteria Enterococcus faecalis have been shown to dominate in persistent periapical lesion and usually recovered from failed root canal treated cases. Moreover, E. faecalis has been reported to withstand the antimicrobial agent and endure potential starvation and resist the antibacterial effect of calcium hydroxide intracanal medication.(omitted)

The main objectives of root canal therapy are cleaning and shaping and then obturating the root canal system in 3 dimensions to prevent reinfection. Many instrumentation techniques and devices, supported by an irrigation system capable of removing pulp tissue remnants and dentin debris, have been proposed to shape root canals. But current regimens in chemomechanical debridement using instrumentation and irrigation with NaOCl are not predictably effective in root canal disinfection. These findings are not surprising because the root canal system is complex and contains numerous ramifications and anatomical irregularities. The microorganisms in root canals not only invade the anatomic irregularities of the root canal system but also are present in the dentinal tubules. Therefore further disinfection with an effective antimicrobial agent may be necessary and it well1mown that use of intracanal medication will lower bacterial count in infected root canals. Calcium hydroxide has a long history of use in endodontics, and more attention has been given to the use of calcium hydroxide as intracanal dressing for the treatment of infected pulp. However, when treatment is completed in one visit, no intracanal medications other than intracanal irrigants are used. Recently, a mixture of a tetracycline isomer, an acid, and a detergent(MTAD), has been introduced as a final rinse for disinfuction of the root canal system. It has been shown that MTAD is able to remove the smear layer with minimal erosive changes on the surface of dentin, and is effective against Enterococcus faecalis, a microorganism resistant to the action of other antimicrobial medications. In another study, the ability of MTAD was investigated to disinfect contaminated root canals with whole saliva and compared its efficacy to that of NaOCl Based on the results, it seems that MTAD is significantly more effective than 5.25% NaOCl in eradicating bacteria from infected root canals. In the cytotoxicity evaluation, MTAD is less cytotoxic than engenol, 3% $H20_2,\;Ca(OH)_2$ paste, 5.25% NaGCl, Peridex, and EDTA and more cytotoxic than 2.63%,1.31% and 0.66% NaOCl. Is it promising or transient?

I. Objectives The purpose of this study was to evaluate the effect of adhesive property on microtensile bond strength and to determine the failure mode. II. Materials and Methods Flat occlusal dentin surfaces were prepared using low-speed diamond saw. The dentin was etched with 37% phosphoric acid. The following adhesives were applied to the etched dentin according to manufacturer's directions；Scotchbond Multi-Purpose in group 1, Primer＆Bond NT in group 2, Scotchbond Multi-Purpose followed by Tetric-flow in group 3. After adhesive application, a cylinder of resin-based composite was built up the occlusal surface.(omitted)

The purpose of this study was to propose a new dental color system through color evaluation of generally used dental composite and porcelain. In this study, Vita Omega 900 dental porcelain and 10 kinds of composite resins were used. A disk specimens(15mm diameter, 4mm thickness) of each material/shade combination were made. Resin compasite was condensed into Teflon mold and pressed between glass plates to flatten the surface. Pressed compasite was polymerized using an Optilux 501(Demetron, USA) for 200 seconds of each side.(중략)

Mechanical removal in decayed teeth has been performed using drill and sharp hand instruments. These methods have some major disadvantages. Namely mechanical preparation often induces pain, local anesthesia and often leads to overextended cavities. Therefore, to avoid these difficulties, a possible alternative method has been introduced for chemo-mechanical excavation of dentin caries lesions on the market. The purpose of this study was to evaluate the efficacy of between traditional mechanical and chemo-mechanical methods(Carisolv).(omitted)

The purpose of this study was to compare the effect of exponential curing method with conventional curing and two step soft start curing method on polymerization shrinkage of composite resins. Three brands of composite resins (Synergy Duo Shade, Z-250, Supreme) and three brands of light curing units (Spectrum 800, Elipar Highlight, Elipar Trillight) were used. In this study, the diameter of specimen was 5.5mm and height 1.6mm and the specimen was cured for 40 seconds. The shrinkage was measured by custom made linometer. The amount of linear polymerization shrinkage recorded in the computer every 0.5 second for 90 seconds. Each group was measured 10 times.(omitted)

I. Objectives The purpose of this study was to compare the marginal microleakage of Prompt L-Pop, AQ Bond, One-Up Bond F, Futurabond, and Xeno III in Class V cavity. II. Materials and Methods One hundred Class V direct resin restorations were placed in the buccal and lingual surfaces of 50 extracted human molars. Each cavity had occlusal margin in enamel and gingival margin in dentin. Teeth were randomly divided into five groups and restored using one of the single step adhesives and composite resins.(omitted)

I. Objectives This study was performed to get some information about micromorphology of subsurface lesion of root caries by observing autofluorescence using confocal laser scanning microscope(CLSM) with minimum sample preparation. II. Materials and Methods Half-cut and 1 mm thick sample of human teeth were prepared to produce artificial root carious lesions. Incipient subsurface lesions were produced under optimal pH and saturity. The lesions were observed by polarized microscopy, CLSM, and back-scattered electron microscope(BSE). Calcium and phosphorus concentrations of the lesions were analyzed by line EDAX. (omitted)

Recently root caries is reported as an increasing oral disease. Many reserchers studied in vivo and in vitro enamel lesions but due to its perticular structure and different chemical composition compared to the enamel, little effort has been made to root caries study. The purpose of this study is to compare and to evaluate the effects of the degree of saturation on the progression of artificial root caries lesion. A total of 8 human premolars without any defects and cracks were selected and were removed cementum and were cleaned with ultrasonic device and pumice without fluoride.(omitted)

I. Objectives This study was done to evaluate whether or not there are any differences in microleakage of the class 5 composite restoration that were filled at the smooth surface and the proximal surface respectively. In addition any differences between small and large-sized restoration were also studied. II. Materials and Methods Total sixty-four class 5 resin restorations, sixteen per group, were made in the permanent teeth. Two-sized cavities, small ($2{\;}{\times}{\;}2{\;}{\times}{\;}1.5{\;}mm$) and large ($4{\;}{\times}{\;}2{\;}{\times}{\;}1.5{\;}mm$), were filled at the smooth surface and the proximal surface each.(omitted)

The purpose of this study was to evaluate the etching effects and bond strength of total etching and self-etching adhesive system on unground enamel using scanning electron microscopy and microtensile bond strength test. The buccal coronal unground enamel from human extracted molars were prepared using low-speed deamond saw. Scotchbond Multi-Purpose(group CM), Clearfil SE Bond(group SE), or Adper Prompt L-pop(group LP) were applied to the prepared teeth, and resin compasite(Z-250) was built up incrementally. Resin tag formation were evaluated by scanning electron microscopy, after removal of enamel surface by acid dissolution and dehydration.(중략)

This study was intended to evaluate the influence of different base materials on the marginal integrity of posterior direct composite resin restorations. Large conventional butt-joint MOD cavity preparations, with margins in enamel and dentin, were prepared in vitro in 24 extracted human mandibular molars and randomly divided into four equal groups. One of three base materials(RMGI, compomer, flowable resin) was placed on the pulpal floors, of the teeth of the groups. One group of teeth, which served as the control group, was not given any base mterial.(중략)

The purpose of this study was to see the occurrence of the epithelial ingrowth in the periapical lesion and its relevance with the clinical symptoms. The subjects of this study included 129 specimens which were obtained from the apical surgery during the year of 2001-2002. The patterns of epithelial ingrowth were classified as either as either lining or networking. The clinical records were reviewed and the relevance between the clinical symptoms and the pattern of the epithelial ingrowth were evaluated under the light microscope.(중략)

I. Objectives In order to examine the immunoresponse of host cells to Enterococcus faecalis, this in vitro study monitored the production of Interleukin-2(IL-2), Interleukin-4(IL-4) and Transforming growth $factor-{\beta}1(TGF-{\beta}1)$ in human lymphocytes. II. Materials and methods Enterococcus faecalis(ATCC29212) strains were used in this study. Strains were grown in 1-liter cultures in 85% N2-10% H2-5% $CO_2$chamber for 3 days at $37^{\circ}C$. The medium used was 3.7% brain heart infusion broth. Bacterial cells harvested from 1-liter cultures were washed, suspended in 20ml of phosphate-buffered saline(PBS). Suspensions of bacterial cells were disrupted by sonication on ice for 5 min. Protein concentration was determined by the Bicinchoninic acid(BCA) protein assay.(omitted)

Autotransplantation is a viable option for the treatment of a missing tooth or for replacement of avulsed and traumatized tooth when there is a donor tooth available. The maintenance of healthy periodontal ligament cells in the donor tooth is one of the most important factors for successful tooth transplantation. The purpose of this study was to evaluate the prognosis and the cause of failure in 182 auto-transplanted teeth. A total of 182 cases which received the auto-transplantation in Department of Conservative dentistry, Yonsei Dental Hospital were included in this study.(omitted)

I. Objectives The aim of this was to evaluate the use possibility of light curing flowable composite resins as a luting agent for translucent fiber posts. II. Materials and Methods 20 single-rooted maxillary central incisors were selected and crown was sectioned below the cemento-enamel junction to obtain a 13 mm ling root. Root canals were filed, cleaned, and shaped to ＃40 with K-file. Prepared canals were filled with gutta percha and AH26 root canal sealer by lateral condensation method. Teeth were than divided into 4 groups of 5 specimens each. In group 1 and 2, the canal space of each root was enlarged with ＃3 DT Light post preparation drill (Bisco, USA) to a depth of 9mm from the cervical.(omitted)

I. Objectives The purpose of this study was to investigate the ultrastructure of sclerotic dentin of non-carious cervical lesions after acid etching. II. Materials and methods Teeth with non-carious cervical lesions were collected and dentinal surfaces were acid etched with 35% phosphoric acid. The micromorphological features of peritubular dentin and intra-tubular calcific structure were observed with scanning electron microscope.(omitted)

I. Objectives Self-etching primer adhesive system is affected to dentin surface conditioning and priming. Especially application time of self-etching primer is very important factor of clinical procedure which has direct influence on smear layer, etching reaction and primer penetration to dentin. This study evaluated the influence of application time of self-etching primers on microtensile bond strength (${\mu}{\;}TBS$) to dentin using three self-etching primer adhesive systems.(omitted)

Laser doppler flowmeter (LDF) has been applied to the measurement of pulpal blood flow (PBF) in human teeth. As far as we searched, the detection area of the pulp in the blood flow measurement has not been clarified, yet. Therefore, the purpose of this study was to obtain information of the detection area in PBF measurement using LDF. The experiments were performed on the artificial blood circulation in extracted human upper central incisors. The apical portions of examined teeth (n=6) were severed and root canals were enlarged from the apical end to the 2mm incisal to the level of enamel-cement junction. An individual resin cap of each tooth was prepared and a hole was drilled 2mm incisal to enamel-cement junction of the labial side of the cap. The measurement probe of LDF (MBF3D, Moor Instrument, UK) was plugged into the hole of the cap. Heparinized human peripheral blood, which was in advance collected and diluted 3 times with physiological saline, was pumped through the apical foramen of the teeth via a silicone tube and a disposable needle (o.d. 0.7mm) and blood flow signals were monitored. The flux signal significantly increased with the enlargement of the root canal to incisal direction (p<0.01, Friedman analysis). The result indicates that the performance of LDF in PBF with human teeth is limited.

Dental follicle is the mesenchymal tissue which surrounds developing tooth germ. During tooth root development, periodontal components such as cementum, periodontal ligament and alveolar bone are considered to be created by progenitors present in the dental follicle. However, little is known about these progenitors. Previously we observed that cultured bovine dental follicle cells (BDFC) contained putative cementoblast progenitors. To further analyze the biology of these cells, we have attempted to immortalize BDFC by expression of the polycomb group protein Bmi-1 and human telomerase reverse transcriptase (hTERT). The BDFC expressing Bmi-1 and hTERT showed extended life span by 90 population doublings more than normal BDFC, and still contained cells with potential to differentiate into cementoblasts upon implantation into immunodeficiency mice. Among them, we established a clonal cell line designated as BCPb8, which formed cemetum-like mineralized tissue reactive to anti-cementum specific monoclonal antibody, 3G9, and expressed mRNA for bone sialoprotein, osteocalcin, osteopontin and type I collagen upon implantation. Thus with the combination of hTERT and Bmi-1, we succeeded in immortalization of cementoblast progenitor in BDFC without affecting differentiation potential. The BCPb8 progenitor cell line could be a useful tool not only to study cementogenesis but also to develop regeneration therapy for periodontitis.