Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Preparation and Characterization of Carrageenase Immobilized onto Polyethyleneimine-Modified Pomelo Peel

  • Qin Yin (College of Biological and Food Engineering, Suzhou University) ;
  • Christopher G. Batbatan (Department of Biology, Central Mindanao University) ;
  • Yongxing Li (College of Ocean Food and Biological Engineering, Jimei University) ;
  • Yonghui Zhang (College of Ocean Food and Biological Engineering, Jimei University) ;
  • Qiuming Yang (College of Ocean Food and Biological Engineering, Jimei University) ;
  • Anfeng Xiao (College of Ocean Food and Biological Engineering, Jimei University)
  • Received : 2023.04.19
  • Accepted : 2023.06.07
  • Published : 2024.01.28
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Abstract

In this study, carrageenase immobilization was evaluated with a concise and efficient strategy. Pomelo peel cellulose (PPC) modified by polyethyleneimine (PEI) using the physical absorption method was used as a carrier to immobilize carrageenase and achieved repeated batch catalysis. In addition, various immobilization and reaction parameters were scrutinized to enhance the immobilization efficiency. Under the optimized conditions, the enzyme activity recovery rate was more than 50% and 4.1 times higher than immobilization with non-modified pomelo peels. The optimum temperature and pH of carrageenase after immobilization by PEI-modified pomelo peel, at 60℃ and 7.5 respectively, were in line with the free enzyme. The temperature resistance was reduced, inconsistent with free enzyme, and pH resistance was increased. A significant loss of activity (46.8%) was observed after reusing it thrice under optimal reaction conditions. In terms of stability, the immobilized enzyme conserved 76.0% of the initial enzyme activity after 98 days of storage. Furthermore, a modest decrease in the kinetic constant (Km) value was observed, indicating the improved substrate affinity of the immobilized enzyme. Therefore, modified pomelo peel is a verified and promising enzyme immobilization system for the synthesis of inorganic solvents.

Keywords

Acknowledgement

The Major Science and Technology Programs and Special Topics of Fujian Province (2020NZ012013), Scientific Research Platform Open Access of Suzhou University Foundation (2016ykf12) and Natural Science research project of the Education Department of Anhui Province (2023AH052217) provided funding for this project.

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