Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Anti-Obesity Effect of Kimchi with Starter Cultures in 3T3-L1 Cells

  • Received : 2023.07.05
  • Accepted : 2023.10.05
  • Published : 2024.01.28
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Abstract

Lactic acid bacteria (LAB) isolated from kimchi have various functions, including antioxidant, anti-inflammation, and anti-obesity activities, and are therefore widely used in the food, pharmaceutical, and medical fields. To date, the health functionalities of LAB have been widely reported; however, those of kimchi fermented with LAB as a starter have rarely been reported. Therefore, research on the selection of LAB with anti-obesity activity and the health functionality of kimchi fermented with LAB is needed. In the present study, LAB with anti-obesity activity were initially selected by measuring the Oil-Red O intensity. Among the four LAB strains, anti-obesity activity was confirmed by measuring cell viability, lipid levels, and lipid accumulation. Then, starter kimchi (SK) was prepared by inoculating selected LABs, and its pH, total acidity, and salinity were compared with those of naturally fermented kimchi (NK). Lastly, anti-obesity activity was also investigated in 3T3-L1 cells. Selected LAB showed no cytotoxicity up to 107 CFU/ml, with Lactobacillus brevis JC7 and Leuconostoc mesenteroides KCKM0828 having higher inhibitory effects on TG, TC content and lipid accumulation. Most SKs showed fermentation properties similar to those of the NK. SKs showed no cytotoxicity at concentrations of up to 1,000 ㎍/ml. SKs showed strong inhibitory effects on TG content, lipid accumulation, and obesity-related gene and protein expressions. Taken together, the utilization of LAB as a starter could improve the health benefits of kimchi.

Keywords

Acknowledgement

This research was supported by the World Institute of Kimchi (KE2201-1 & KE(C)2203) and funded by the Ministry of Sciences and ICT, Republic of Korea.

References

  1. Tang H, Huang W, Yao YF. 2023. The metabolites of lactic acid bacteria: classification, biosynthesis and modulation of gut microbiota. Microb. Cell 10: 49-62.
  2. Cha J, Kim YB, Park SE, Lee SH, Roh SW, Son HS, et al. 2023. Does kimchi deserve the status of a probiotic food? Crit. Rev. Food Sci. Nutr. 30: 1-14.
  3. Chen Y, Yang B, Zhao J, Ross RP, Stanton C, Zhang H, et al. 2022. Exploiting lactic acid bacteria for colorectal cancer: a recent update. Crit. Rev. Food Sci. Nutr. 18: 1-17.
  4. Chen Q, Wang H, Wang G, Zhao J, Chen H, Lu X, et al. 2022. Lactic acid bacteria: a promising tool for menopausal health management in women. Nutrients 14: 4466.
  5. Lv W, Zhang D, He T, Liu Y, Shao L, Lv Z, et al. 2023. Combination of Lactobacillus plantarum improves the effects of tacrolimus on colitis in a mouse model. Front. Cell. Infect. Microbiol. 13: 1130820.
  6. Jia DJ, Wang QW, Hu YY, He JM, Ge QW, Qi YD, et al. 2022. Lactobacillus johnsonii alleviates colitis by TLR1/2-STAT3 mediated CD206+  macrophagesIL-10 activation. Gut Microbes 14: 2145843.
  7. Han KJ, Lee NK, Yu HS, Park H, Paik HD. 2022. Anti-adipogenic effects of the probiotic Lactiplantibacillus plantarum KU15117 on 3T3-L1 adipocytes. Probiotics Antimicrob. Proteins 14: 501-509.
  8. Lee CS, Park MH, Kim BK, Kim SH. 2021. Antiobesity effect of novel probiotic strains in a mouse model of high-fat diet-induced obesity. Probiotics Antimicrob. Proteins. 13: 1054-1067.
  9. Lee J, Jang JY, Kwon MS, Lim SK, Kim N, Lee J, et al. 2018. Mixture of two Lactobacillus plantarum strains modulates the gut microbiota structure and regulatory T cell response in diet-induced obese mice. Mol. Nutr. Food Res. 62: e1800329.
  10. Ban OH, Lee M, Bang WY, Nam EH, Jeon HJ, Shin M, et al. 2023. Bifidobacterium lactis IDCC 4301 exerts anti-obesity effects in high-fat diet-fed mice model by regulating lipid metabolism. Mol. Nutr. Food Res. 67: e2200385.
  11. Cho YG, Yang YJ, Yoon YS, Lee ES, Lee JH, Jeong Y, et al. 2022. Effect of MED-02 containing two probiotic strains, Limosilactobacillus fermentum MG4231 and MG4244, on body fat reduction in overweight or obese subjects: a randomized, multicenter, double-blind, placebo-controlled study. Nutrients 14: 3583.
  12. Gu Y, Xiao X, Pan R, Zhang J, Zhao Y, Dong Y, et al. 2021. Lactobacillus plantarum dy-1 fermented barley extraction activates white adipocyte browning in high-fat diet-induced obese rats. J. Food Biochem. 45: e13680.
  13. Hwang JE, Kim KT, Paik HD. 2019. Improved antioxidant, anti-inflammatory, and anti-adipogenic properties of hydroponic ginseng fermented by Leuconostoc mesenteroides KCCM 12010P. Molecules 24: 3359.
  14. Kim NH, Moon PD, Kim SJ, Choi IY, An HJ, Myung NY, et al. 2008. Lipid profile lowering effect of soypro fermented with lactic acid bacteria isolated from Kimchi in high-fat diet-induced obese rats. Biofactors 33: 49-60.
  15. Wang LC, Pan TM, Tsai TY. 2018. Lactic acid bacteria-fermented product of green tea and Houttuynia cordata leaves exerts anti-adipogenic and anti-obesity effects. J. Food Drug Anal. 26: 973-984.
  16. Hwang H, Lee HJ, Lee MA, Sohn H, Chang YH, Han SG, et al. 2020. Selection and characterization of Staphylococcus hominis subsp. hominis WiKim0113 isolated from kimchi as a starter culture for the production of natural pre-converted nitrite. Food Sci. Anim. Resour. 40: 512-526.
  17. Kang JY, Lee M, Song JH, Choi EJ, Kim DU, Lim SK, et al. 2022. Lactic acid bacteria strains used as starters for kimchi fermentation protect the disruption of tight junctions in the caco-2 cell monolayer model. J. Microbiol. Biotechnol. 32: 1583-1588.
  18. Seo H, Bae JH, Kim G, Kim SA, Ryu BH, Han NS. 2021. Suitability analysis of 17 probiotic type strains of lactic acid bacteria as starter for kimchi fermentation. Foods 10: 1435.
  19. Seo H, Seong H, Kim GY, Jo YM, Cheon SW, Song Y, et al. 2021. Development of anti-inflammatory probiotic Limosilactobacillus reuteri EFEL6901 as kimchi starter: in vitro and in vivo evidence. Front. Microbiol. 12: 760476.
  20. Park SJ, Lee MJ, Choi YJ, Lee MA, Min SG, Seo HY, et al. 2023. Effect of the addition of maltodextrin on metabolites and microbial population during kimchi fermentation. J. Food Sci. Technol. 60: 2153-2159.
  21. Lee MA, Choi YJ, Kim YS, Chon SY, Chung YB, Park SH, et al. 2022. Effects of salt type on the metabolites and microbial community in kimchi fermentation. Heliyon 8: e11360.
  22. Jung MJ, Kim J, Lee SH, Whon TW, Sung H, Bae JW, et al. 2022. Role of combinated lactic acid bacteria in bacterial, viral, and metabolite dynamics during fermentation of vegetable food, kimchi. Food Res. Int. 157: 111261.
  23. Lee M, Yun YR, Choi EJ, Song JH, Kang JY, Kim D, et al. 2023. Anti-obesity effect of vegetable juice fermented with lactic acid bacteria isolated from kimchi in C57BL/6J mice and human mesenchymal stem cells. Food Funct. 14: 1349-1356.
  24. Sorensen HM, Rochfort KD, Maye S, MacLeod G, Brabazon D, Loscher C, et al. 2022. Exopolysaccharides of lactic acid bacteria: production, purification and health benefits towards functional food. Nutrients 14: 2938.
  25. Lee JJ, Choi YJ, Lee MJ, Park SJ, Oh SJ, Yun YR, et al. 2020. Effects of combining two lactic acid bacteria as a starter culture on model kimchi fermentation. Food Res. Int. 36: 109591.
  26. Lee KH, Song JL, Park ES, Ju J, Kim HY, Park KY. 2015. Anti-obesity effects of starter fermented kimchi on 3T3-L1 adipocytes. Prev. Nutr. Food Sci. 20: 298-302.
  27. Lee M, Song JH, Shim WB, Chang JY. 2020. DNAzyme-based quantitative loop-mediated isothermal amplification for strain-specific detection of starter kimchi fermented with Leuconostoc mesenteroides WiKim32. Food Chem. 333: 127343.
  28. Yun YR, Park SH, Kim IH. 2019. Antioxidant effect of kimchi supplemented with Jeju citrus concentrate and its antiobesity effect on 3T3-L1 adipocytes. Food Sci. Nutr. 7: 2740-2746.
  29. Jung MY, Lee SH, Lee M, Song JH, Chang JY. 2017. Lactobacillus allii sp. nov. isolated from scallion kimchi. Int. J. Syst. Evol. Microbiol. 67: 4936-4942.
  30. Lee CS, Park MH, Kim SH. 2022. Selection and characterization of probiotic bacteria exhibiting antiadipogenic potential in 3T3-L1 preadipocytes. Probiotics Antimicrob. Proteins 14: 72-86.
  31. Kim JY, Park EJ, Lee HJ. 2022. Ameliorative effects of Lactobacillus plantarum HAC01 lysate on 3T3-L1 adipocyte differentiation via AMPK activation and MAPK inhibition. Int. J. Mol. Sci. 23: 5901.
  32. Lee YS, Park EJ, Park GS, Ko SH, Park J, Lee YK, et al. 2021. Lactiplantibacillus plantarum ATG-K2 exerts an anti-obesity effect in high-fat diet-induced obese mice by modulating the gut microbiome. Int. J. Mol. Sci. 22: 12665.
  33. Lee JJ. 2021. Effect of a kimchi-derived combined starter on the fermentation process of kimchi Korean J. Food Preserv. 28: 141-146.
  34. Yun YR, Choi YJ, Kim YS, Chon SY, Lee MA, Chung YB, et al. 2023. Antioxidant and anti-inflammatory effects of solar salt brined kimchi. Food Sci. Biotechnol. 32: 679-687.
  35. Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, Gonzalez FJ, et al. 2002. C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway. Genes Dev. 16: 22-26.
  36. Sung YY, Son E, Im G, Kim DS. 2020. Herbal combination of Phyllostachys pubescens and Scutellaria baicalensis inhibits adipogenesis and promotes browning via AMPK activation in 3T3-L1 adipocytes. Plants (Basel) 9: 1422.
  37. Mostafa AM, Hamdy NM, El-Mesallamy HO, Abdel-Rahman SZ. 2015. Glucagon-like peptide 1 (GLP-1)-based therapy upregulates LXR-ABCA1/ABCG1 cascade in adipocytes. Biochem. Biophys. Res. Commun. 468: 900-905.
  38. Lee JH, Woo KJ, Kim MA, Hong J, Kim J, Kim SH, et al. 2022. Heat-killed Enterococcus faecalis prevents adipogenesis and high fat diet-induced obesity by inhibition of lipid accumulation through inhibiting C/EBP-α and PPAR-γ in the insulin signaling pathway. Nutrients 14: 1308.
  39. Ree J, Kim JI, Lee CW, Lee J, Kim HJ, Kim SC, et al. 2021. Quinizarin suppresses the differentiation of adipocytes and lipogenesis in vitro and in vivo via downregulation of C/EBP-beta/SREBP pathway. Life Sci. 287: 120131.