Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Investigation of Contamination Levels of CI Toxins in Imported Shellfish Products Available in South Korea

국내 수입산 패류의 Cyclic Imine (CI) 독소에 대한 오염도 조사 분석

  • Young-Sang Kim (Department of Marine Life Science, Jeju National University) ;
  • HyeWon Kim (Asia Glycomics Reference Site, Chungnam National University) ;
  • JaeYoung Yu (Asia Glycomics Reference Site, Chungnam National University) ;
  • Myung Jin Oh (Asia Glycomics Reference Site, Chungnam National University) ;
  • Nari Seo (Asia Glycomics Reference Site, Chungnam National University) ;
  • Changsun Choi (Department of Food and Nutrition, College of Biotechnology and Agriculture Resource, Chung-Ang University) ;
  • Hyun Joo An (Asia Glycomics Reference Site, Chungnam National University) ;
  • You Jin Jeon (Department of Marine Life Science, Jeju National University)
  • 김영상 (제주대학교 해양생명과학과) ;
  • 김혜원 (충남대학교 아시아-태평양 당분석 연구교육센터) ;
  • 유재영 (충남대학교 아시아-태평양 당분석 연구교육센터) ;
  • 오명진 (충남대학교 아시아-태평양 당분석 연구교육센터) ;
  • 서나리 (충남대학교 아시아-태평양 당분석 연구교육센터) ;
  • 최창순 (중앙대학교 식품공학부 식품영양전공) ;
  • 안현주 (충남대학교 아시아-태평양 당분석 연구교육센터) ;
  • 전유진 (제주대학교 해양생명과학과)
  • Received : 2024.09.06
  • Accepted : 2024.10.08
  • Published : 2024.10.30
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Abstract

The detection of high levels of cyclic imine (CI) toxins, noted for their neurotoxic effects, in imported shellfish products represents a significant public health concern for South Korea. In this study, we investigated the levels of CI contamination in these imported products. Specifically, we focused on spirolides (SPX-C), pinnatoxin (PnTX), and gymnodimine-A (gym-A), utilizing advanced LC/MS/MS analytical techniques. Samples collected from a range of imported shellfish revealed substantial contamination, with SPX-C being detected at 227.84 ppt in New Zealand mussels, PnTX-G at 80.42 ppt in Chilean mussels, and GYM-A at 118.79 ppt in New Zealand mussels. These findings are particularly alarming, given that the concentrations of CIs in shellfish from certain countries often exceed those found in South Korean products, posing a potentially significant risk to public health when these contaminated products are imported. On the basis of our findings, we advocate the urgent implementation of stringent regulatory measures to control and mitigate the health hazards associated with these toxins in shellfish products. Additionally, we emphasize the importance of ongoing monitoring and continuous research to gain a better understanding of these risks and to develop effective strategies for their management, which will thereby contribute to protecting consumer health in the context of increasing global seafood trade.

이 연구는 신경독성을 나타내는 cyclic imine (CI) 독소의 한국 내 수입되는 패류 내의 오염 수준을 분석한 것으로, LC/MS/MS를 통해서 spirolide, pinnatoxin, gymnodimine 유도체 7종을 대상으로 분석하였다. 총 21개의 수입산 패류를 수집하여 분석한 결과, 뉴질랜드산 홍합에서 SPX C가 227.84 ppt, 칠레산 홍합에서 PnTX G가 80.42 ppt, 뉴질랜드산 홍합에서 Gym A가 118.79 ppt로 검출되었다. 이러한 결과는 수입산 패류에서 CI 독소가 초래할 잠재적인 위협의 가능성을 나타내며, 소비자 건강 보호를 위하여 지속적인 모니터링의 필요성을 시사한다. 또한 이러한 독소가 포함된 패류 제품에 대한 규제 조치의 시행이 필요함을 시사하며, 이를 보다 잘 이해하고 관리하기 위한 지속적인 연구가 필요하다.

Keywords

Acknowledgement

본 연구는 2023년도 식품의약품안전처의 연구개발비(20163MFDS641)로 수행되었으며, 이에 감사드립니다.

References

  1. Harnedy, P.A., FitzGerald, R.J., Bioactive peptides from marine processing waste and shellfish: a review. J. Funct. Foods, 4, 6-24 (2012).
  2. Gerssen, A., Pol-Hofstad, I.E., Poelman, M., Mulder, P.P.J., Van den Top, H.J., de Boer, J., Marine toxins: chemistry, toxicity, occurrence and detection, with special reference to the dutch situation. Toxins, 2, 878-904 (2010).
  3. Mafra, L.L., de Souza, D.A., Menezes, M., Schramm, M.A., Hoff, R., Marine biotoxins: latest advances and challenges toward seafood safety, using Brazil as a case study. Curr. Opin. Food Sci., 53, 101078 (2023).
  4. Otero, A., Chapela, M.J., Atanassova, M., Vieites, J.M., Cabado, A.G., Cyclic imines: chemistry and mechanism of action: a review. Chem. Res. Toxicol., 24, 1817-29 (2011).
  5. Sarah, C., Finch, D., Harwood, T., Boundy, M.J., Selwood A.I., A review of cyclic imines in shellfish: worldwide occurrence, toxicity and assessment of the risk to consumers. Mar. Drugs, 22, 129 (2024).
  6. Stirling, D.J., Survey of historical New Zealand shellfish samples for accumulation of gymnodimine. New Zeal. J. Mar. Freshw. Res., 35, 851-857 (2001).
  7. McCarron, P., Wright, E., Quilliam, M.A., Liquid chromatography/mass spectrometry of domoic acid and lipophilic shellfish toxins with selected reaction monitoring and optional confirmation by library searching of product ion spectra. J. AOAC Int., 97, 316-324 (2019).
  8. Bacchiocchi, S., Siracusa, M., Campacci, D., Ciriaci, M., Dubbini, A., Tavoloni, T., Stramenga, A., Gorbi, S., Piersanti, A., Cyclic imines (Cis) in mussels from north-central adriatic sea: first evidence of gymnodimine a in Italy. Toxins, 12, 370 (2020).
  9. Gill, S., Murphy, M., Clausen, J., Richard, D., Quilliam, M., MacKinnon, S., LaBlanc, P., Mueller, R., Pulido, O., Neural injury biomarkers of novel shellfish toxins, spirolides: a pilot study using immunochemical and transcriptional analysis. Neurotoxicology, 24, 593-604 (2003).
  10. Sleno, L., Chalmers, M.J., Volmer, D.A., Structural study of spirolide marine toxins by mass spectrometry. Anal. Bioanal. Chem., 378, 977-86 (2004).
  11. Aasen, J.A., Hardstaff, W., Aune, T., Quilliam, M.A., Discovery of fatty acid ester metabolites of spirolide toxins in mussels from norway using liquid chromatography/tandem mass spectrometry. Rapid. Commun. Mass. Spectrom., 20, 1531-1537 (2006).
  12. Aasen, J., MacKinnon, S.L., LeBlanc, P., Walter, J.A., Hovgaard, P., Aune, T., Quilliam, M.A., Detection and identification of spirolides in Norwegian shellfish and plankton. Chem. Res. Toxicol., 18, 509-15 (2005).
  13. Otero, P., Alfonso, A., Rodriguez, P., Rubiolo, J.A., Cifuentes, J.M., Bermudez, R., Vieytes, M.R., Botana, L.M., Pharmacokinetic and toxicological data of spirolides after oral and intraperitoneal administration. Food Chem. Toxicol., 50, 232-237 (2012).
  14. Richard, E.A.D., Cembella, A., Quilliam, M., Investigations into the toxicology and pharmacology of spirolides, a novel group of shellfish toxins, in Proceedings of the ninth international conference on harmful algal blooms, pp. 383-386 (2000).
  15. EFSA panel on contaminants in the food chain (CONTAM), Scientific opinion on marine biotoxins in shellfish - cyclic imines (spirolides, gymnodimines, pinnatoxins and pteriatoxins). EFSA J., 8, 1628 (2010).
  16. Rhodes, L., Smith, K., Selwood, A., McNabb, P., van Ginkel, R., Holland, P., Munday, R., Production of pinnatoxins by a peridinoid dinoflagellate isolated from Northland, New Zealand. Harmful Algae, 9, 384-389 (2010).
  17. Selwood, A.I., Miles, C.O., Wilkins, A.L., van Ginkel, R., Munday, R., Rise, F., McNabb, P., Isolation, structural determination and acute toxicity of pinnatoxins E, F and G. J. Agr. Food Chem., 58, 6532-6542 (2010).
  18. Munday, R., Towers, N.R., Mackenzie, L., Beuzenberg, V., Holland, P.T., Miles, C.O., Acute toxicity of gymnodimine to mice. Toxicon, 44, 173-178 (2004).
  19. Rhodes, L., Smith, K., Selwood, A., McNabb, P., Molenaar, S., Munday, R., Wilkinson, C., Hallegraeff, G., Production of pinnatoxins E, F and G by scrippsielloid dinoflagellates isolated from franklin harbour, South Australia. New Zeal. J. Mar. Freshw. Res., 45, 703-709 (2011).
  20. Takada, N., Umemura, N., Suenaga, K., Uemura, D., Structural determination of pteriatoxins a, B and C, extremely potent toxins from the bivalve pteria penguin. Tetrahedron. Lett., 42, 3495-3497 (2001b).
  21. Otero, P., Vale, C., Boente-Juncal, A., Costas, C., Louzao, M.C., Botana, L.M., Detection of cyclic imine toxins in dietary supplements of green lipped mussels (Perna canaliculus) and in shellfish mytilus chilensis. Toxins, 12, 613 (2020).
  22. Moreiras, G., Leao, J.M., Gago-Martinez, A., Analysis of cyclic imines in mussels (mytilus galloprovincialis) from Galicia (Nw Spain) by Lc-Ms/Ms. Int. J. Environ. Res. Public Health, 17, 281 (2020).
  23. Seo, N., Jo, H.Y., Lee, S.G., Kim, H.J., Oh, M.J., Kim, Y.S., Ro, S., Jeon, Y.J., An, H.J., An enhanced Lc-Mrm-Ms platform for sensitive and simultaneous quantification of cyclic imines in shellfish. J. Chromatogr. B, 1229, 123883 (2023).
  24. Norambuena, L., Mardones, J.I., Emerging phycotoxins in the chilean coast: first localized detection of the neurotoxic cyclic imine pinnatoxin-G in shellfish banks. Mar. Pollut. Bull., 190, 114878 (2023).
  25. Cangini, M., Dall'Ara, S., Rubini, S., Bertasi, B., Rizzi, P., Dell'Orfano, G., Milandri, S., Manfredini, S., Baldini, E., Vertuani, S., First report of Pinnatoxin-G (Pntx-G) in a marine-coastal area of the adriatic sea associated with the presence of the dinoflagellate vulcanodinium rugosum. Mar. Drugs, 22, 122 (2024).
  26. Ji, Y., Che, Y., Wright, E.J., McCarron, P., Hess, P., Li, A., Fatty acid ester metabolites of gymnodimine in shellfish collected from China and in mussels (mytilus galloprovincialis) exposed to karenia selliformis. Harmful Algae, 92, 101774 (2020).
  27. Ji, Y., Hu, Y., Song, J.L., Chen, H.D., Li, A.F., Characteristics of components and regional distribution of lipophilic shellfish toxins in bivalves cultured along the Chinese coast in spring. Chinese Fishery Quality and Standards, 8, 15-24 (2018).