Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Fertilizing capacity of cryopreserved sperm of Chirostoma jordani (Woolman, 1894)

  • Received : 2023.10.20
  • Accepted : 2024.01.26
  • Published : 2024.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

The genus Chirostoma is endemic from the Mesa Central of Mexico. It is conformed of 18 species and six subspecies. Five species are in some category of risk, because of this, Chirostoma jordani is an excellent model species to implement biotechnologies like gametes cryopreservation. Aim of present study was to evaluate fertilizing capacity of cryopreserved C. jordani sperm, as alternative to conservation and assisted reproduction in this specie and genus. Males and females were collected from wild Atlangatepec dam stock, Tlaxcala State, Mexico. Seminal quality was evaluated in fresh and cryopreserved semen with three cryoprotective agents (CPAs): 10% dimethyl sulfoxide (DMSO), 10% methanol (MeOH), 14% ethylene glycol (EG) and it was determined its post-thaw fertilizing capacity. Sperm motility percentage decreased during cryopreservation process (p < 0.05). There were not significant differences in post-thaw motility percentage between EG (53.5 ± 1.9%) and MeOH (53.3 ± 1.3%), but DMSO (50.3 ± 0.5%) was significantly different (p < 0.05). Results showed that 0.2 μL fresh semen were enough to fertilize 100% oocytes (n = 60). 10 μL DMSO and 5 μL MeOH and EG cryopreserved semen were necessary to fertilize oocytes 100% (n = 60) (p < 0.05). Cryopreservation and fertilization protocol for C. jordani sperm was efficient and it could be used for its assisted reproduction.

Keywords

Acknowledgement

Present research is part of posdoctoral stay of first author (BGJD, CVU: 566816).

References

  1. Ahn JY, Park JY, Lim HK. Effects of different diluents, cryoprotective agents, and freezing rates on sperm cryopreservation in Epinephelus akaara. Cryobiology. 2018;83:60-4. https://doi.org/10.1016/j.cryobiol.2018.06.003
  2. Barbour CD. A biogeographical history of Chirostoma (Pisces: Atherinidae): a species flock from the Mexican plateau. Copeia. 1973;3:533-56. https://doi.org/10.2307/1443118
  3. Borges A, Siqueira DR, Jurinitz DF, Zanini R, Amaral F, Grillo ML, et al. Biochemical composition of seminal plasma and annual variations in semen characteristics of jundia Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiol Biochem. 2005;31:45-53. https://doi.org/10.1007/s10695-005-4742-8
  4. Bozkurt Y, Yavas I. Effect of different straw volumes and thawing rates on post-thaw quality and fertilization ability of cryopreserved common carp (Cyprinus carpio) sperm. J Limnol Freshw Fish Res. 2017;3:25-31. https://doi.org/10.17216/limnofish.277835
  5. Bustamante-Gonzalez JD, Cortes-Garcia A, Avalos Rodriguez A, Figueroa Lucero G, Arenas Rios E, Gutierrez MR. Semen evaluation in Chirostoma jordani (Woolman, 1894) and Chirostoma humboldtianum (Valenciennes, 1835), Mexican native species (Atheriniformes: Atherinopsidae). Int J Aquatic Sci. 2018;9:38-43.
  6. Bustamante-Gonzalez JD, Rodriguez-Gutierrez M, CortesGarcia A, Arenas-Rios E, Figueroa-Lucero G, Avalos-Rodriguez A. Cryopreservation of Chirostoma jordani sperm, fish model for the conservation of the genus in Mexico. J Appl Ichthyol. 2021;37:278-84. https://doi.org/10.1111/jai.14151
  7. Bustamante-Gonzalez JD, Rodriguez-Gutierrez M, CortesGarcia A, Arenas-Rios E, Figueroa-Lucero G, Avalos-Rodriguez A. Fisiologia y criopreservacion del espermatozoide en teleosteos. AquaTIC. 2019;53:1-17.
  8. Cabrita E, Martinez-Paramo S, Gavaia PJ, Riesco MF, Valcarce DG, Sarasquete MP, et al. Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture. 2014;432:389-401. https://doi.org/10.1016/j.aquaculture.2014.04.034
  9. Ciereszko A, Dietrich GJ, Nynca J, Dobosz S, Zalewski T. Cryopreservation of rainbow trout semen using a glucose-methanol extender. Aquaculture. 2014;420:275-81. https://doi.org/10.1016/j.aquaculture.2013.11.014
  10. Di lorio M, Esposito S, Rusco G, Roncarati A, Miranda M, Gibertoni PP, et al. Semen cryopreservation for the Mediterranean brown trout of the Biferno River (Molise-Italy): comparative study on the effects of basic extenders and cryoprotectants. Sci Rep. 2019;9:9703.
  11. Diaz R, Lee-Estevez M, Quinones J, Dumorne K, Short S, Ulloa-Rodriguez P, et al. Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation. Anim Reprod Sci. 2019;204:50-9. https://doi.org/10.1016/j.anireprosci.2019.03.004
  12. Du C, Han YL, Shi YX, Zhu JQ. Cryopreservation of Plagiognathops microlepis sperm. Cryobiology. 2018;85:105-12. https://doi.org/10.1016/j.cryobiol.2018.08.012
  13. Figueroa E, Lee-Estevez M, Valdebenito I, Watanabe I, Oliveira RPS, Romero J, et al. Effects of cryopreservation on mitochondrial function and sperm quality in fish. Aquaculture. 2019;511:634190.
  14. Gironi B, Kahveci Z, McGill B, Lechner Bob Dan, Pagliara S, Metz J, et al. Effect of DMSO on the mechanical and structural properties of model and biological membranes. Biophys J 2020;119:274-86. https://doi.org/10.1016/j.bpj.2020.05.037
  15. Hernandez-Rubio MC, Figueroa-Lucero G, Barriga-Sosa IDLA, Arredondo-Figueroa JL, Castro-Barrera T. Early development of the shortfin silverside Chirostoma humboldtianum (Valenciennes, 1835) (Atheriniformes: Atherinopsidae). Aquaculture. 2006;261:1440-6. https://doi.org/10.1016/j.aquaculture.2006.08.048
  16. Judycka S, Slowinska M, Nynca J, Liszewska E, Dobosz S, Ciereszko A. Oxidative stress in cryopreserved semen of sex-reversed female and normal male rainbow trout. Aquaculture. 2020;528:1-8. https://doi.org/10.1016/j.aquaculture.2020.735531
  17. Kommisrud E, Myromslien FD, Stenseth EB, Zeremichael TT, Hofman N, Grevle I, et al. Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation. Theriogenology. 2020;151:58-65. https://doi.org/10.1016/j.theriogenology.2020.04.008
  18. Kumari P, Kashyap HK. DMSO induced dehydration of heterogeneous lipid bilayers and its impact on their structures. J Chem Phys. 2019;151:215103-12. https://doi.org/10.1063/1.5127852
  19. Lake PE, Ravie O. An exploration of cryoprotective compounds for fowl spermatozoa. Br Poult Sci. 1984;25:145-50. https://doi.org/10.1080/13632758408454852
  20. Lee-Estevez M, Herrera L, Diaz R, Beltran J, Figueroa E, Dumorne K, et al. Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: relation with post-thaw motility. Anim Reprod Sci. 2019;209:106133.
  21. Lopez-Lopez E, Sedeno-Diaz JE, Perozzi F. Lipid peroxidation and acetylcholinesterase activity as biomarkers in the Black sailfin Goodeis, Girardinichthys viviparous (Bustamante) exposed to water from Lake Xochimilco (Mexico). Aquat Ecosyst Health Manag. 2006;9:379-85. https://doi.org/10.1080/14634980600886871
  22. Malcervelli DM, Torres P, Suhevic JF, Cisale H, Fischman ML. Effect of different glycerol concentrations on phosphatidylserine translocation and mitochondrial membrane potential in chilled boar spermatozoa. Cryobiology. 2020;95:97-102. https://doi.org/10.1016/j.cryobiol.2020.05.012
  23. Miller RR, Minckley WL, Norris SN. Freshwater fishes of Mexico. Chicago, IL: The University of Chicago Press; 2005.
  24. Notman R, Noro M, O'Malley B, Anwar J. Molecular basis for dimethyl sulfoxide (DMSO) action on lipid membranes. J Am Chem Soc. 2006;128:13982-3. https://doi.org/10.1021/ja063363t
  25. Nynca J, Slowinska M, Judycka S, Ciereszko A. Maladaptation of trout spermatozoa to fresh water is related to oxidative stress and proteome changes. Reproduction. 2019;157:485-99. https://doi.org/10.1530/REP-19-0012
  26. Sandoval-Vargas L, Dumorne K, Contreras P, Farias JG, Figueroa E, Risopatron J, et al. Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): effect on sperm function, oxidative stress and fertilizing capacity. Aquaculture. 2020;533:736151.
  27. Schrader AM, Cheng CY, Israelachvili JN, Han S. Communication: contrasting effects of glycerol and DMSO on lipid membrane surface hydration dynamics and forces. J Chem Phys. 2016;145:041101.
  28. Secretaria del Medio Ambiente y Recursos Naturales [SEMARNAT]. Norma Oficial Mexicana NOM-059-SEMARNAT-2019, Proteccion ambiental-Especies nativas de Mexico de flora y fauna silvestres-Categorias de riesgo y especificaciones para su inclusion, exclusion o cambio-Lista de especies en 2010 [Internet]. Diario Oficial De La Federacion. 2019 [cited 2024 Feb 2]. https://www.dof.gob.mx/nota_detalle.php?codigo=5578808&fecha=14/11/2019#gsc.tab=0
  29. Tekin N, Seccer S, Akcay E, Bozkurt Y, Kayam S. Effects of glycerol additions on post-thaw fertility of frozen rainbow trout sperm, with an emphasis on interaction between extender and cryoprotectant. J Appl Ichthyol. 2007;23:60-3. https://doi.org/10.1111/j.1439-0426.2006.00792.x
  30. Valipour J, Nashtaei MS, Khosravizadeh Z, Mahdavinezhad F, Nekoonam S, Esfandyari S, et al. Effect of sulforaphane on apoptosis, reactive oxygen species and lipids peroxidation of human sperm during cryopreservation. Cryobiology. 2021;99:122-30. https://doi.org/10.1016/j.cryobiol.2020.11.012
  31. Viveiros ATM, Nascimento AF, Leal AC, Goncalves ACS, Ordao LH, Cosson J. Methyl glycol, methanol and DMSO effects on post-thaw motility, velocities, membrane integrity and mitochondrial function of Brycon orbignyanus and Prochilodus lineatus (Characiformes) sperm. Fish Physiol. Biochem. 2015;41:193-201. https://doi.org/10.1007/s10695-014-0016-7