Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Fabrication and Characterization of Lactate Oxidase-catalase-mitochondria Electrode

젖산 산화효소-카탈라아제-미토콘드리아 전극 제작 및 특성 분석

  • Ke Shi (Department of Chemical Engineering and RIGET) ;
  • Keerthi Booshan Manikandan (Department of Chemical Engineering and RIGET) ;
  • Young-Bong Choi (Department of Cosmedical & Materials, College of Bio-convergence, Dankook University) ;
  • Chang-Joon Kim (Department of Chemical Engineering and RIGET)
  • 시키 (경상국립대학교 화학공학과 및 그린에너지 연구소) ;
  • 마니칸단 키에르티 부샨 (경상국립대학교 화학공학과 및 그린에너지 연구소) ;
  • 최영봉 (단국대학교 코스메디컬소재학과) ;
  • 김창준 (경상국립대학교 화학공학과 및 그린에너지 연구소)
  • Received : 2024.06.18
  • Accepted : 2024.07.07
  • Published : 2024.08.01
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Abstract

The lactate electrode can be utilized either as an electrode for lactate sensor to monitor the patient's health status, stress level, and athlete's fatigue in real time or lactate fuel cell. In this study, we fabricated a high-performance electrode composed of lactate oxidase, catalase, and mitochondria, and investigated the surface analysis and electrochemical properties of this electrode. Carbon paper modified with single-walled carbon nanotubes (CP-SWCNT) had significantly improved electrical conductivity compared to before modification. The electrode to which lactate oxidase, catalase, and mitochondria were attached (CP-SWCNT-LOx-Cat-Mito) produced a higher current than the electrode to which lactate oxidase and catalase were attached. The amount of reduction current produced by the bilirubin oxidase (BOD)-attached electrode (CP-SWCNT-BOD) was greatly affected by the presence or absence of oxygen in the electrolyte. The fuel cell composed of CP-SWCNT-LOx-Cat-Mito (anode) and CP-SWCNT-BOD (cathode) produced maximum power (29 ㎼/cm2) at a discharge current density of 133 ㎂/cm2. From this study, we had proved that mitochondria is essential for improving lactate sensor and fuel cell performance.

젖산 전극은 환자의 건강상태와 스트레스 수준, 및 운동선수의 피로도를 실시간으로 모니터링하는 젖산 센서 또는 젖산 연료전지 전극으로 활용될 수 있다. 본 연구에서는 젖산 산화효소, 카탈라아제, 미토콘드리아로 구성된 고성능 전극을 제작하고 전극의 표면분석 및 전기화학적 특성을 조사하였다. 단일벽 탄소나노튜브로 개질된 탄소종이(CPSWCNT)는 개질 전보다 전기 전도성이 크게 향상되었다. 젖산 산화효소, 카탈라아제, 그리고 미토콘드리아가 부착된 전극(CP-SWCNT-LOx-Cat-Mito)은 젖산 산화효소와 카탈라아제가 부착된 전극에 비하여 많은 전류를 생산하였다. 빌리루빈 산화효소(BOD)가 부착된 전극(CP-SWCNT-BOD)이 생산하는 환원전류량은 전해질의 산소 존재 유무에 따라 크게 영향을 받았다. CP-SWCNT-LOx-Cat-Mito (anode)와 CP-SWCNT-BOD (cathode)로 구성된 연료전지는 133 ㎂/cm2로 방전 시 0.2 V의 셀 전위를 유지하며 29 ㎼/cm2의 전력을 생산하였다. 본 연구결과는 미토콘드리아가 젖산 센서 및 연료전지 성능 향상에 필수적인 생체물질임을 시사한다.

Keywords

Acknowledgement

이 성과는 정부의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(NRF-2020R1F1A1054433, 2021RIS-003)

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