Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Change in the Gut Microbiota of Lactating Sows and Their Piglets by Inclusion of Dietary Spray-Dried Plasma in Sow Diets

  • Jeong Jae Lee (Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • Hyunjin Kyoung (Division of Animal and Dairy Science, Chungnam National University) ;
  • Jin Ho Cho (Division of Food and Animal Science, Chungbuk National University) ;
  • Kyeong Il Park (Division of Animal and Dairy Science, Chungnam National University) ;
  • Yonghee Kim (Division of Animal and Dairy Science, Chungnam National University) ;
  • Jinmu Ahn (Division of Animal and Dairy Science, Chungnam National University) ;
  • Jeehwan Choe (Korea National of Agriculture and Fisheries) ;
  • Younghoon Kim (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Hyeun Bum Kim (Department of Animal Resources Science, Dankook University) ;
  • Minho Song (Division of Animal and Dairy Science, Chungnam National University)
  • Received : 2023.11.01
  • Accepted : 2023.12.07
  • Published : 2024.03.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to investigate the effects of dietary spray-dried plasma (SDP) on the gut microbiota of lactating sows and their piglets. A total of 12 sows were randomly assigned to one of two dietary treatment groups in a completely randomized design. The treatments were a sow diet based on corn and soybean meal (CON), and a CON diet with an added 1% SDP. The sows were fed the dietary treatments from d 30 before farrowing to weaning (d 28). The fecal samples of three sows from each treatment and two of their randomly selected piglets were collected to verify their fecal microbiota. There were no differences in the alpha diversity and distinct clustering of the microbial communities in the sows and their piglets when SDP was added to the sow diets from late gestation to weaning. The fecal microbiota of the lactating sows and their piglets showed a higher relative abundance of the phylum Bacteroidota and genus Lactobacillus and Ruminococcus and showed a lower relative abundance of the phylum Bacillota and genus Bacteroides, Escherichia/Shigella, and Clostridium in the sows fed the SDP diet than those fed the CON diet. Overall, these results show that the addition of SDP to the sow diet during lactation altered the gut environment with positive microbial composition changes. These results were similar in the nursing piglets, suggesting that the control of the sow diets during lactation may contribute to the intestinal health and growth in piglets after weaning.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (grant no. NRF-2021R1I1A3046876).

References

  1. Farmer C, Edwards SA. 2022. Review: improving the performance of neonatal piglets. Animal 16: 100350. 
  2. Ren P, Yang XJ, Kim JS, Menon D, Baidoo SK. 2017. Effect of different feeding levels during three short periods of gestation on sow and litter performance over two reproductive cycles. Anim. Reprod. Sci. 177: 42-55.  https://doi.org/10.1016/j.anireprosci.2016.12.005
  3. Mateo RD, Wu G, Bazer FW, Park JC, Shinzato I, Kim SW. 2007. Dietary l-arginine supplementation enhances the reproductive performance of gilts. J. Nutr. 137: 652-656.  https://doi.org/10.1093/jn/137.3.652
  4. Muhizi S, Cho S, Palanisamy T, Kim IH. 2022. Effect of dietary salicylic acid supplementation on performance and blood metabolites of sows and their litters. J. Anim. Sci. Technol. 64: 707-716.  https://doi.org/10.5187/jast.2022.e25
  5. Wu G, Bazer FW, Johnson GA, Knabe DA, Burghardt RC, Spencer TE, et al. 2011. Important roles for L-glutamine in swine nutrition and production. J. Anim. Sci. 89: 2017-2030.  https://doi.org/10.2527/jas.2010-3614
  6. Yang YX, Heo S, Jin Z, Yun JH, Choi JY, Yoon SY, et al. 2009. Effects of lysine intake during late gestation and lactation on blood metabolites, hormones, milk composition and reproductive performance in primiparous and multiparous sows. Anim. Reprod. Sci. 112: 199-214.  https://doi.org/10.1016/j.anireprosci.2008.04.031
  7. Upadhaya SD, Seok WJ, Kumar SS, van der Veen RH, Kim IH. 2023. Marine derived Ca-Mg complex supplementation basal diet during four subsequent parities improved longevity and performance of sows and their litters. J. Anim. Sci. Technol. 65: 562-578.  https://doi.org/10.5187/jast.2022.e121
  8. Mackie RI, Sghir A, Gaskins HR. 1999. Developmental microbial ecology of the neonatal gastrointestinal tract. Am. J. Clin. Nutr. 69: 1035s-1045s.  https://doi.org/10.1093/ajcn/69.5.1035s
  9. Nowland TL, Kirkwood RN, Pluske JR. 2022. Can early-life establishment of the piglet intestinal microbiota influence production outcomes? Animal 16: 100368. 
  10. Tannock GW, Fuller R, Pedersen K. 1990. Lactobacillus succession in the piglet digestive tract demonstrated by plasmid profiling. Appl. Environ. Microbiol. 56: 1310-1316.  https://doi.org/10.1128/aem.56.5.1310-1316.1990
  11. Siggers RH, Siggers J, Boye M, Thymann T, Molbak L, Leser T, et al. 2008. Early administration of probiotics alters bacterial colonization and limits diet-induced gut dysfunction and severity of necrotizing enterocolitis in preterm pigs. J. Nutr. 138: 1437-1444.  https://doi.org/10.1093/jn/138.8.1437
  12. Vasquez R, Oh JK, Song JH, Kang DK. 2022. Gut microbiome-produced metabolites in pigs: a review on their biological functions and the influence of probiotics. J. Anim. Sci. Technol. 64: 671-695.  https://doi.org/10.5187/jast.2022.e58
  13. Kim K, Kim B, Kyoung H, Liu Y, Campbell JM, Song M, et al. 2021. Dietary spray-dried plasma supplementation in late-gestation and lactation enhanced productive performance and immune responses of lactating sows and their litters. J. Anim. Sci. Technol. 63: 1076-1085.  https://doi.org/10.5187/jast.2021.e83
  14. Touchette KJ, Carroll JA, Allee GL, Matteri RL, Dyer CJ, Beausang LA, et al. 2002. Effect of spray-dried plasma and lipopolysaccharide exposure on weaned pigs: I. Effects on the immune axis of weaned pigs1. J. Anim. Sci. 80: 494-501.  https://doi.org/10.2527/2002.802494x
  15. Torrallardona D, Conde MR, Badiola I, Polo J, Brufau J. 2003. Effect of fishmeal replacement with spray-dried animal plasma and colistin on intestinal structure, intestinal microbiology, and performance of weanling pigs challenged with Escherichia coli K991. J. Anim. Sci. 81: 1220-1226.  https://doi.org/10.2527/2003.8151220x
  16. Zhang Y, Chen DW, Yu B, He J, Yu J, Mao XB, et al. 2015. Spray-dried chicken plasma improves intestinal digestive function and regulates intestinal selected microflora in weaning piglets1. J. Anim. Sci. 93: 2967-2976.  https://doi.org/10.2527/jas.2014-8820
  17. NRC. 2012. Nutrient requirements of swine. 11th rev. ed. Ed. Natl Acad Press, Washington, DC. 
  18. Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. 2013. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl. Environ. Microbiol. 79: 5112-5120.  https://doi.org/10.1128/AEM.01043-13
  19. Hugerth LW, Wefer HA, Lundin S, Jakobsson HE, Lindberg M, Rodin S, et al. 2014. DegePrime, a program for degenerate primer design for broad-taxonomic-range PCR in microbial ecology studies. Appl. Environ. Microbiol. 80: 5116-5123.  https://doi.org/10.1128/AEM.01403-14
  20. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25: 402-408.  https://doi.org/10.1006/meth.2001.1262
  21. Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M, et al. 2012. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res. 41: e1. 
  22. Edgar RC. 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26: 2460-2461.  https://doi.org/10.1093/bioinformatics/btq461
  23. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. 2010. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods 7: 335-336.  https://doi.org/10.1038/nmeth.f.303
  24. Daneshmand A, Sharma NK, Dao TH, Barekatain R, Swick RA, Wu SB. 2023. Spray-dried porcine plasma enhances feed efficiency, intestinal integrity, and immune response of broilers challenged with necrotic enteritis. Poult. Sci. 102: 102431. 
  25. Moreto M, Miro L, Amat C, Polo J, Manichanh C, Perez-Bosque A. 2020. Dietary supplementation with spray-dried porcine plasma has prebiotic effects on gut microbiota in mice. Sci. Rep. 10: 2926. 
  26. Wexler HM. 2007. Bacteroides: the good, the bad, and the Nitty-Gritty. Clin. Microbiol.Rev. 20: 593-621.  https://doi.org/10.1128/CMR.00008-07
  27. Schwiertz A, Taras D, Schafer K, Beijer S, Bos NA, Donus C, et al. 2010. Microbiota and SCFA in lean and overweight healthy subjects. Obesity 18: 190-195.  https://doi.org/10.1038/oby.2009.167
  28. Jumpertz R, Le DS, Turnbaugh PJ, Trinidad C, Bogardus C, Gordon JI, et al. 2011. Energy-balance studies reveal associations between gut microbes, caloric load, and nutrient absorption in humans. Am. J. Clin. Nutr. 94: 58-65.  https://doi.org/10.3945/ajcn.110.010132
  29. Lilburn, Schmidt, Breznak. 1999. Phylogenetic diversity of termite gut spirochaetes. Environ. Microbiol. 1: 331-345.  https://doi.org/10.1046/j.1462-2920.1999.00043.x
  30. Niu Q, Li P, Hao S, Zhang Y, Kim SW, Li H, et al. 2015. Dynamic distribution of the gut microbiota and the relationship with apparent crude fiber digestibility and growth stages in pigs. Sci. Rep. 5: 9938. 
  31. Felis GE, Pot B. 2014. The family Lactobacillaceae, pp. 245-247. Lactic Acid Bacteria, Ed., 
  32. Ryu S, Kim K, Cho DY, Kim Y, Oh S. 2022. Complete genome sequences of Lactococcus lactis JNU 534, a potential food and feed preservative. J. Anim. Sci. Technol. 64: 599-602.  https://doi.org/10.5187/jast.2022.e34
  33. Kim JY, Kim JY, Kim H, Moon EC, Heo K, Shim JJ, et al. 2022. Immunostimulatory effects of dairy probiotic strains Bifidobacterium animalis ssp. lactis HY8002 and Lactobacillus plantarum HY7717. J. Anim. Sci. Technol. 64: 1117-1131.  https://doi.org/10.5187/jast.2022.e84
  34. Valeriano VDV, Balolong MP, Kang DK. 2017. Probiotic roles of Lactobacillus sp. in swine: insights from gut microbiota. J. Appl. Microbiol. 122: 554-567.  https://doi.org/10.1111/jam.13364
  35. Yang Y, Zhao X, Le MHA, Zijlstra R, Ganzle M. 2015. Reutericyclin producing Lactobacillus reuteri modulates development of fecal microbiota in weanling pigs. Front. Microbiol. 6: 762. 
  36. Zhang L, Xu YQ, Liu HY, Lai T, Ma JL, Wang JF, et al. 2010. Evaluation of Lactobacillus rhamnosus GG using an Escherichia coli K88 model of piglet diarrhoea: effects on diarrhoea incidence, faecal microflora and immune responses. Vet. Microbiol. 141: 142-148.  https://doi.org/10.1016/j.vetmic.2009.09.003
  37. Harris JK, El Kasmi KC, Anderson AL, Devereaux MW, Fillon SA, Robertson CE, et al. 2014. Specific microbiome changes in a mouse model of parenteral nutrition associated liver injury and intestinal inflammation. PLoS One 9: e110396. 
  38. Kaakoush NO. 2015. Insights into the role of Erysipelotrichaceae in the human host. Front. Cell. Infect. Microbiol. 5: 84. 
  39. Wong JMW, de Souza R, Kendall CWC, Emam A, Jenkins DJA. 2006. Colonic health: fermentation and short chain fatty acids. J. Clin. Gastroenterol. 40: 235-243.  https://doi.org/10.1097/00004836-200603000-00015
  40. Gu X, Sim JXY, Lee WL, Cui L, Chan YFZ, Chang ED, et al. 2022. Gut Ruminococcaceae levels at baseline correlate with risk of antibiotic-associated diarrhea. iScience 25: 103644. 
  41. Collado MC, Rautava S, Aakko J, Isolauri E, Salminen S. 2016. Human gut colonisation may be initiated in utero by distinct microbial communities in the placenta and amniotic fluid. Sci. Rep. 6: 23129. 
  42. Nowland TL, Kirkwood RN, Pluske JR. 2022. Review: can early-life establishment of the piglet intestinal microbiota influence production outcomes? Animal 16: 100368. 
  43. Liu B, Zhu X, Cui Y, Wang W, Liu H, Li Z, et al. 2021. Consumption of dietary fiber from different sources during pregnancy alters sow gut microbiota and improves performance and reduces inflammation in sows and piglets. mSystems 6: 10.1128/msystems.00591-00520. 
  44. Sampath V, Park JH, Pineda L, Han Y, Cho S, Kim IH. 2022. Sows fed with synergistic blend of short- and medium chain organic acid has a carryover effect on post-weaning growth rate. J. Anim. Sci. Technol. 64: 302-311.  https://doi.org/10.5187/jast.2022.e11
  45. Beaumont M, Cauquil L, Bertide A, Ahn I, Barilly C, Gil L, et al. 2021. Gut microbiota-derived metabolite signature in suckling and weaned piglets. J. Proteome Res. 20: 982-994.  https://doi.org/10.1021/acs.jproteome.0c00745
  46. Ryu S, Lee JJ, Mun D, Kim SR, Choe J, Song M, et al. 2022. The ingestion of dietary prebiotic alternatives during lactation promotes intestinal health by modulation of gut microbiota. J. Microbiol. Biotechnol. 32: 1454-1461.  https://doi.org/10.4014/jmb.2208.08017
  47. Lee JJ, Song M, Kyoung H, Park KI, Ryu S, Kim Y, et al. 2022. Effects of dietary carbohydrases on fecal microbiome composition of lactating sows and their piglets. J. Microbiol. Biotechnol. 32: 776-782. https://doi.org/10.4014/jmb.2203.03026