Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Evaluation of antioxidant activity, zebrafish embryo toxicity, and regenerative efficacy of Symphoricarpos albus

Symphoricarpos albus의 항산화능과 Zebrafish 배아 독성 및 재생 효능 평가

  • Chanwoo Lee (Miglim Co., Ltd) ;
  • HyeYeon Heo (Dept. of Cosmetics Engineering, College of Technology Sciences. Mokwon University) ;
  • Myunsoo Kim (Future Technology Research Center, ICBIO Co, Ltd) ;
  • YoungPyo Jang (College of Pharmacy, Kyung Hee University) ;
  • Bo Ae Kim (Dept. of Cosmetics Engineering, College of Technology Sciences. Mokwon University)
  • 이찬우 ((주)미그림) ;
  • 허혜연 (목원대학교 화학과 화장품전공) ;
  • 김면수 ((주)ICBIO) ;
  • 장영표 (경희대학교 약학대학) ;
  • 김보애 (목원대학교 화장품공학과)
  • Received : 2023.12.27
  • Accepted : 2023.04.15
  • Published : 2024.04.30
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Abstract

This study compared and evaluated the antioxidant activities of Symphoricarpos albus(S. albus) extract and fermented extract. Antioxidant activity was measured by DPPH radical scavenging, FRAP, and ABTS. Concentrations were measured at 200, 100, 50, and 10 ㎍/mL, and antioxidant activity increased in a concentration-dependent manner. S. albus leaves fermented extracts had the highest antioxidant activity. And this study evaluated the safety and tail regeneration of S. albus extract using zebrafish model embryos. Zebrafish are in the spotlight as an alternative animal and can be used for cosmetic research. Zebrafish embryos were collected and evaluated for coagulation rate, hatching rate, and cardiotoxicity. As a result, it was toxic at concentrations above 100 ㎍/ml. The tail was cut and the regenerative effect was observed for 3 days. As a result, from 72 hours, S. albus 200ug/ml leaf extract showed a 17% regenerative effect compared to the control group. These results suggest that S. albus can be used as a natural material for antioxidant and regeneration for skin improvement.

본 연구는 Symphoricarpos albus(S. albus) 추출물과 발효 추출물을 대상으로 zebrafish 배아를 이용하여 천연 화장품 소재로서의 유효성 평가 및 꼬리지느러미 재생력을 비교 평가하였다. 이를 위한 S. albus 추출물의 항산화 활성은 10-200 ㎍/mL 농도에서 DPPH radical scavenging, FRAP activity, ABTS+ radical scavenging을 진행하였으며, 모두 농도 의존적인 radical 소거 활성을 보이고, S. albus 잎 추출물에서 가장 높을 항산화 활성을 나타냈다. Zebrafish는 현재 각광받고 있는 실험 대체 동물로서 수많은 화장품 연구에 활용되고 있으며, 본 연구는 zebrafish 배아를 채취하여 응고율, 부화율, 심장 독성을 평가하였다. 그 결과 발효 추출물의 경우 100 ㎍/mL 이상의 농도에서는 독성을 나타내는 것을 확인하였다. 재생 효능을 평가하기 위해 zebrafish 꼬리지느러미를 절단하였고, 3일 동안 상처 회복력을 관찰하였다. 그 결과 72시간 부터 S. albus 잎 추출물 200 ㎍/mL에서 대조군 대비 17%의 재생 효과를 나타내었다. 이러한 결과는 S. albus가 피부 개선용 항산화 및 재생을 위한 천연소재로서 활용 가능한 것으로 사료된다.

Keywords

Acknowledgement

본 성과물(논문)은 농촌진흥청에서 시행한 농업실용화기술R&D지원사업(과제번호: RS-2023-00215852)의 지원에 의해 이루어진 것임.

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