Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

A Study on the Physiological Activity of Cosmetics in Bupleurum falcatum L. Extract

시호 추출물의 화장품 생리활성에 관한 연구

  • Il-Young Cho (Department of Cosmetic science, Hoseo University) ;
  • Yong-Sub Yi (Department of Cosmetic biotechnology, Hoseo University) ;
  • Yong-Hwa Lee (Department of Cosmetic biotechnology, Hoseo University)
  • 조일영 (호서대학교 화장품과학과) ;
  • 이용섭 (호서대학교 화장품생명공학부) ;
  • 이용화 (호서대학교 화장품생명공학부)
  • Received : 2023.03.21
  • Accepted : 2024.02.29
  • Published : 2024.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Bupleurum falcatum L. is one of the medicinal crops and has excellent pharmacological activities such as reducing fever, sedation, inhibition of hepatopathy, anti-inflammatory, and anticancer. Currently, it is used for food or medicine, but the use as a cosmetic is insufficient, so the suitability and use value as a cosmetic material were reviewed. Using the Bupleurum falcatum L. extract, polyphenol, flavonoid, antioxidant, and anti-inflammatory tests were conducted. The total polyphenol content was 14.71±0.16 mg·TAE/g, and the total flavonoid content was 1.42±0.05 mg·QE/g. The antioxidant activity showed excellent activity in a concentration-dependent activative, and the NO expression inhibition ability was 61.21% at 1,000 ㎍/mL, confirming that the anti-inflammatory activity was excellent. Through this study, the antioxidant and anti-inflammatory effects of Bupleurum falcatum L. extract are excellent, so it is considered to be of sufficient value as a natural material for cosmetics.

시호는 약용작물의 하나로 해열, 진정, 간 장애 억제, 항염, 항암 등 우수한 약리 활성을 지니고 있다. 현재 식품 또는 약용으로 사용되고 있지만 화장품으로서의 사용은 미비하여 화장품 소재로서의 적합성 및 이용 가치를 검토하였다. 시호 추출물을 이용하여 폴리페놀, 플라보노이드, 항산화, 항염 실험을 진행하였다. 폴리페놀 함량은 14.71±0.16 mg·TAE/g, 플라보노이드 함량은 1.42±0.05 mg·QE/g를 나타내었다. 항산화 활성은 농도 의존적으로 우수한 활성을 보였으며, NO 발현 억제능은 1,000 ㎍/mL에서 61.21%의 저해율을 나타내어 항염 활성이 뛰어남을 확인하였다. 본 연구를 통해 시호 추출물의 항산화, 항염 효능이 뛰어나기에 화장품의 천연 소재로서 충분한 가치가 있다고 본다.

Keywords

References

  1. Rural Development Administration. Agricultural Technology Guide 007, pp. 127-133, (2019).
  2. BC Moon, BK Choo, YI Ji, TS Yoon, AY Lee, MS Cheon, BB Kim and HK Kim. "Molecular Authentication and Phylogenetic Relationship of Bupleurum Species by the rDNA-ITS Sequences" Kor. J. Herbology, Vol.24, No.3, pp. 59-68, (2009).
  3. National Institute of Food and Drug Safety Evaluation. A casebook of ingredient profile by main ingredient of herbal medicine preparation, 220-01, pp. 95-101, (2022).
  4. BM Kim, KD Yoon, KR Han and JW Kim. "Determination of saikosa ponin derivatives in Bupleuri Radix using HPLC-ELSD" J. Kor. Pharm. Sci., Vol.38, No.1, pp. 57-61, (2008). https://doi.org/10.4333/KPS.2008.38.1.057
  5. DH Kim, TS Park and JH Son. "Anti-wrinkle Activities Verification of Buplerum falcatum Extracts on CCD-986sk" J. Appl. Biol. Chem., Vol.52, No.2, pp. 183-187, (2015). https://doi.org/10.3839/jabc.2015.029
  6. KS Kim, ST Lee, NS Seoung, JI Lee and YA Chae. "Comparison of Analytical Methods for Saikosaponins in Bupleurum falcatum L." Korean J. Medicinal Crop. Sci., Vol.3, No.3, pp. 226-232, (1995).
  7. KS Kim, ST Lee and YA Chae. "Medicinal Components in Bupleurum Species" Korean J. Crop Sci., Vol.41, No.1, pp. 123-144, (1996).
  8. DS Han and DK Lee. "Separation and Determination of Saikosaponins in Bupleuri Radix with HPLC" Kor. J. Pharmacogn., Vol.16, No.3, pp. 175-179, (1985).
  9. HJ Jeong. "Comparison on Morphology and saikosaponin Contents of Bupleurum falcatum Produced in Korea and China" Korean J. Plant. Res., Vol.11, No.3, pp. 283-289, (1998).
  10. FJ Baur and LG Ensminger. The Association of Official Analytica l Chemists (AOAC). Journal of the American Oil Chemists' Society. Vol.54, No.4, pp. 171-172, (1977). https://doi.org/10.1007/BF02670789
  11. JS Park. "Application of Pine Needle Extract as Cosmetic Material" J. Digit. Converg., Vol.17, No.7, pp. 395-400, (2019).
  12. MIN Moreno, MI Isla, AR Sampietro and M Vattuone. "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina" J. Ethnopharmacol., Vol.71, No.1-2, pp. 109-114, (2000). https://doi.org/10.1016/S0378-8741(99)00189-0
  13. MS Blois. "Antioxidant determination by the use of a stable free radical" Nature, Vol.181, No.4617, pp. 1199-1200, (1958). https://doi.org/10.1038/1811199a0
  14. R Re, N Pellegrini, A Proteggente, A Pannala, M Yang, and RC Evans. "Antioxidant activity applying an im proved ABTS radical cation decolorization assay" Free radical biology &medicine, Vol.26, No.9-10, pp. 1231-1237, (1999). https://doi.org/10.1016/S0891-5849(98)00315-3
  15. P Ngamwongsatit, PP Banada, W Panbangred and AK Bhunia "WST-1-based cell cytotoxicity assay as substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line" J. Microbiol. Methods, Vol.73, No.3, pp. 211-215, (2008). https://doi.org/10.1016/j.mimet.2008.03.002
  16. LC Green, DA Wagner, J Glogowski, PL Skipper, JS Wishnok, and SR Tannenbaum. "Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids" Analytical Biochemistry, Vol.126, No.1, pp. 131-139, (1982). https://doi.org/10.1016/0003-2697(82)90118-X
  17. EB Byun, WY Park, DH Ahn, YC Yoo, C Park, WJ Park, BS Jang, EH Byun and NY Sung. "Comparison Study of Three Varieties of Red Peppers in Terms of Total Polyphenol, Total Flavonoid Contents, and Antioxidant Activities" J. Korean Soc. Food Sci. Nutr., Vol.45, No.5, pp. 765-770, (2016). https://doi.org/10.3746/jkfn.2016.45.5.765
  18. YS Park. "Antioxidative Activities and Contents of Polyphenolic Compound of Medicinal Herb Extracts" J. East Asian Soc. Diet. Life, Vol.12, No.1, pp. 23-31, (2002).
  19. GY Jang, HY Kim, SH Lee, YR Kang, IG Hwang, KS Woo, TS Kang, JS Lee and HS Jeong. "Effects of Heat Treatment and Extraction Method on Antioxidant Activity of Several Medicinal Plants" J. Korean Soc. Food Sci. Nutr., Vol.41, No.7, pp. 914-920, (2012). https://doi.org/10.3746/jkfn.2012.41.7.914
  20. YJ Moon and CD Kim. "Antioxidant, antiinflammatory and antimicrobial activity of Sophora flavescens ethanol and hydrothermal extracts" J. Korea Soc. Beauty Art,, Vol.18, No.1, pp. 91-103, (2017). https://doi.org/10.18693/jksba.2017.18.1.91
  21. EH Park and IH Lee. "The Effect of Consumer Decision Type on the Consumption Value of Vegan Cosmetics" J. Kor. Soc. Cosmetol., Vol.27, No.2, pp. 442-454, (2021). https://doi.org/10.52660/JKSC.2021.27.2.442
  22. GY Jang, HY Kim, SH Lee, YR Kang, IG Hwang, KS Woo, TS Kang, JS Lee and HS Jeong. "Effects of Heat Treatment and Extraction Method on Antioxidant Activity of Several Medicinal Plants" J. Korean Soc. Food Sci. Nutr., Vol.41, No.7, pp. 914-920, (2012). https://doi.org/10.3746/jkfn.2012.41.7.914
  23. SA Kang, JA Han, KH Jang and R Choue. "DPPH Radical Scavenger Activity and Antioxidant Effects of Cham-Dang-Gui (Angelica gigas)" J. Korean Soc. Food Sci. Nutr., Vol.33, No.7, pp. 1112-1118, (2004). https://doi.org/10.3746/jkfn.2004.33.7.1112
  24. JH Song, HS Song and GL Lee. "Antioxidant and Elastase Inhibitory Effects of Equisetum hyemale Extract" Journal of Naturopathy. Vol.10, No.2, pp. 86-92, (2021). https://doi.org/10.33562/JN.2021.10.2.3
  25. CS Park. "Antioxidative and Nitrite Scavenging Abilities of Medicinal Plant Extracts" Korean J. Food Preserv., Vol.12, No.6, pp. 631-636, (2005).
  26. JY Choi, MK Jo, YM Goo, HK Kim, JW Shin, DY Kim, HJ Kim, EJ Lee, NH Kim and YJ Cho. "Antioxidant Activities of Phenolic Compounds from Medicinal Plants (Hibiscus esculentus, Cirsium japonicum, Zizania latifolia and Kalopanax pictus)" Current research on agriculture and life sciences, Vol.33, No.2, pp. 57-63, (2015). https://doi.org/10.14518/crals.2015.33.2.010
  27. YJ Woo, SR Shin and JY Hong. "Study on antioxidant and physiological activities of extract from Ligularia fischeri by extraction methods" Korean J. Food Preserv., Vol.24, No.8, pp. 1113-1121, (2017). https://doi.org/10.11002/kjfp.2017.24.8.1113
  28. JH Shin, JR Kang, MJ Kang and JH Shin. "Physiological Activity of Five Kinds of Medicinal Plant Extracts with Various Solvents and Their Composites" J. Life Sci., Vol.28, No.3, pp. 320-330, (2018).
  29. DoGenBio Co., LTD. EZ-CYTOX cell viability, proliferation, cytotoxicity assay kit.
  30. JH Yoon, SG Park, JY Park, KS Seo, KC Woo and Lee CE Lee. "Antioxidant and Anti-inflammatory Effects of Bletilla striata Reichenbach fil. Fractions as Cosmetic" J. Life Sci., Vol.23, No.9, pp. 1073-1078, (2013). https://doi.org/10.5352/JLS.2013.23.9.1073