한국어병학회지 (Journal of fish pathology)

  • 제37권1호
  • /
  • Pages.37-48
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  • 2024
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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In vitro antimicrobial properties of Bacillus subtilis KCTC 1326 for fish bacterial disease management

  • Ji-Yoon Park (Department of Aquatic Life Medical Sciences, Summon University) ;
  • In-Joo Shin (Department of Aquatic Life Medical Sciences, Summon University) ;
  • So-Ri Han (Fishcare laboratory) ;
  • Sung-Hyun Kim (Fishcare laboratory) ;
  • Youhee Kim (Department of Smart Aquaculture, Gangwon State University) ;
  • Se Ryun Kwon (Department of Aquatic Life Medical Sciences, Summon University)
  • 투고 : 2024.05.24
  • 심사 : 2024.06.12
  • 발행 : 2024.06.30
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초록

This study evaluated in vitro antimicrobial properties of Bacillus subtilis KCTC 1326 as an environmentally friendly alternative to antibiotics. B. subtilis KCTC 1326 was characterized on biochemical properties and antibiotics susceptibility. It exhibited antimicrobial effects against all 12 species of fish bacteria used in this experiment. Among them, the largest antibacterial zone was observed for Streptococcus parauberis (34 mm), while the smallest antibacterial zone was observed for Citrobacter freundii (8 mm). Additionally, in the co-culture inhibitory assay of B. subtilis and Edwardsiella piscicida, the growth of E. piscicida was suppressed with increasing concentrations of B. subtilis KCTC 1326, with complete inhibition observed at 107 and 108 CFU/mL of B. subtilis KCTC 1326 after 24 hours of incubation. Moreover, at 48 hours of incubation, the growth of E. piscicida was inhibited across all concentration ranges of B. subtilis KCTC 1326. Therefore, this study indicated the utilizing of B. subtilis KCTC 1326 as an antimicrobial for controlling fish bacterial diseases.

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과제정보

This work was supported by the National Research Foundation of Korea grant (NRF-2023R1A2C1008182) and the Korea Innovation Foundation grant (2023-DD-UP-0007) funded by the Ministry of Science and ICT (MSIT).

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