한국어병학회지 (Journal of fish pathology)

  • 제37권1호
  • /
  • Pages.49-60
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  • 2024
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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난배양성(viable but non-culturable; VBNC) Edwardsiella piscicida의 특성 연구

Characterization of viable but non-culturable (VBNC) Edwardsiella piscicida

  • 김아현 (부경대학교 수산생명의학과) ;
  • 이윤항 (부경대학교 수산생명의학과) ;
  • 노형진 (부경대학교 수산생명의학과) ;
  • 허영웅 (부경대학교 수산생명의학과) ;
  • 김남은 (부경대학교 수산생명의학과) ;
  • 김도형 (부경대학교 수산생명의학과)
  • Ahyun Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Yoonhang Lee (Department of Aquatic Life Medicine, Pukyong National University) ;
  • HyeongJin Roh (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Young-Ung Heo (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Nameun Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Do-Hyung Kim (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2024.05.04
  • 심사 : 2024.06.03
  • 발행 : 2024.06.30
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초록

A viable but non-culturable (VBNC) state is a survival strategy adopted by bacteria when faced with unfavorable environmental conditions, rendering them unable to grow on nutrient agar while maintaining low metabolic activity. This study explored the impact of temperature and nutrient availability on inducing VBNC state in Edwardsiella piscicida, the most important bacterial fish pathogen, and assessed its pathogenicity at VBNC state. E. piscicida was suspended in filtered sterile seawater and exposed to three different temperatures (4, 10, and 25℃) to induce the VBNC state. Subsequently, the induced VBNC cells were subjected to resuscitation by either raising the temperature to 28℃ or inoculating them in brain heart infusion broth supplemented with 1% NaCl. A propidium monoazide (PMA)-qPCR method was also developed to selectively quantify live (VBNC or culturable) E. piscicida cells. The results showed that the bacteria entered the VBNC state after approximately 1 month at 4℃ and 25℃, and 2 months at 10℃. The VBNC E. piscicida cells were successfully revived within 3 days in a nutrient-rich environment at 28℃, highlighting the significance of temperature and nutrition in inducing and resuscitating the VBNC state. In pathogenicity tests, resuscitated E. piscicida cells exhibited high pathogenicity in olive flounder comparable to cultured bacteria, while VBNC cells showed no signs of infection, suggesting they are unlikely to resuscitate in fish. In conclusion, this study contributes to our understanding of fish pathogen ecology by investigating the characteristics of the VBNC state under varying temperature and nutrition conditions.

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과제정보

이 논문은 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구임(20210257, 넙치 SPF(특정 병원체 무감염) 종자생산 기술개발).

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