한국어병학회지 (Journal of fish pathology)

  • 제37권1호
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  • Pages.25-36
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  • 2024
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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2019년부터 2023년까지 국내에서 분리된 참돔이리도바이러스의 계통 분류 및 항원 결정기 예측

Phylogenetic analysis and antigenic determinant prediction of red sea bream iridovirus isolated in Korea from 2019 to 2023

  • 김국현 (부경대학교 수산생명의학과) ;
  • 민준규 (부경대학교 수산생명의학과) ;
  • 정현도 (부경대학교 수산생명의학과) ;
  • 김광일 (부경대학교 수산생명의학과)
  • Guk Hyun Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Joon Gyu Min (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Hyun Do Jeong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kwang Il Kim (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2024.05.13
  • 심사 : 2024.05.27
  • 발행 : 2024.06.30
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초록

In this study, we analyzed the phylogenetic classification, epitope prediction, and pathogenicity of red sea bream iridovirus (RSIV) isolated from rock bream between 2019 and 2023. Phylogenetics based on genes encoding MCP and ATPase indicated that all five RSIV isolates belonged to RSIV subtype II. The deduced amino acid sequence of the MCP for the amplicons (1362 bp) obtained from RSIV isolates had a length of 453 amino acids. Among these, the amino acid sequences of the RSIV-19, 21, 22, and 23 isolates showed 100% identity, while the RSIV-20 isolate showed 99.78% identity with one residue difference at position 306. As a result of antigenicity analysis based on amino acid sequence, the antigenicity score of the RSIV-20 isolate was 0.6386 and the other RSIV isolates were 0.6365. Additionally, the prediction of their antigenic determinants resulted in a total of 17 identical antigenic plots. When each RSIV was inoculated into rock bream, no significant differences were observed with 100% cumulative mortality in all groups. This study provides data on the potential for genetic variation of RSIV isolated in the same marine area over the past five years, and the antigenicity and pathogenicity results of each isolate are expected to be useful information for selecting future vaccine strains.

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과제정보

이 논문은 2022년도 정부(교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업임(No. 2022R1I1A2064371)

참고문헌

  1. Chinchar, V. G., Hick, P., Ince, I. A., Jancovich, J. K., Marschang, R., Qin, Q., Subramaniam, K., Waltzek, T. B., Whittington, R., Williams, T., Zhang, Q. & ICTV Report Consortium. (2017). ICTV virus taxonomy profile: Iridoviridae. Journal of General Virology, 98(5), 890-891. https://doi.org/10.1099/jgv.0.000818
  2. Do, J. W., Cha, S. J., Kim, J. S., An, E. J., Park, M. S., Kim, J. W., Kim, Y. C., Park, M. A. & Park, J. W. (2005). Sequence variation in the gene encoding the major capsid protein of Korean fish iridoviruses. Archives of virology, 150, 351-359. https://doi.org/10.1007/s00705- 004-0424-6
  3. Doytchinova, I. A. & Flower, D. R. (2007). VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC bioinformatics, 8, 1-7. https://doi.org/10.1186/1471-2105-8-4
  4. Dong, H. T., Jitrakorn, S., Kayansamruaj, P., Pirarat, N., Rodkhum, C., Rattanarojpong, T., Senapin, S. & Saksmerprome, V. (2017). Infectious spleen and kidney necrosis disease (ISKND) outbreaks in farmed barramundi (Lates calcarifer) in Vietnam. Fish & Shellfish Immunology, 68, 65-73. https://doi.org/10.1016/ j.fsi.2017.06.054
  5. Fu, X., Li, N., Lai, Y., Liu, L., Lin, Q., Shi, C., Huang, Z. & Wu, S. (2012). Protective immunity against iridovirus disease in mandarin fish, induced by recombinant major capsid protein of infectious spleen and kidney necrosis virus. Fish & Shellfish Immunology, 33(4), 880-885. https://doi.org/10.1016/j.fsi.2012.07.012
  6. Fu, Y., Li, Y., Fu, W., Su, H., Zhang, L., Huang, C., Weng, S., Yu, F., He, J. & Dong, C. (2021). Scale drop disease virus associated yellowfin seabream (Acanthopagrus latus) ascites diseases, Zhuhai, Guangdong, southern China: the first description. Viruses, 13(8), 1617. https://doi. org/10.3390/v13081617
  7. Fu, W., Li, Y., Fu, Y., Zhang, W., Luo, P., Sun, Q., Yu, F., Weng, S., Li, W., He, J. & Dong, C. (2023). The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatus. Microbiology Spectrum, 11(3), e04495-22. https://doi.org/10.1128/spectrum.04495-22
  8. Girisha, S. K., Puneeth, T. G., Nithin, M. S., Kumar, B. N., Ajay, S. K., Vinay, T. N., Suresh, T., Venugopal, M. N. & Ramesh, K. S. (2020). Red sea bream iridovirus disease (RSIVD) outbreak in Asian seabass (Lates calcarifer) cultured in open estuarine cages along the west coast of India: First report. Aquaculture, 520, 734712. https://doi.org/10.1016/j.aquaculture.2019.734712
  9. Girisha, S. K., Kushala, K. B., Nithin, M. S., Puneeth, T. G., Naveen Kumar, B. T., Vinay, T. N., Suresh, T., Ajay, S. K., Venugopal, M. N. & Ramesh, K. S. (2021). First report of the infectious spleen and kidney necrosis virus (ISKNV) infection in ornamental fishes in India. Transboundary and emerging diseases, 68(2), 964-972. https://doi.org/10.1111/tbed.13793
  10. Greenbaum, J. A., Andersen, P. H., Blythe, M., Bui, H. H., Cachau, R. E., Crowe, J., Davies, M., Kolaskar, A. S., Lund, O., Morrison, S., Mumey, B., Ofran, Y., Pellequer, J. L., Pinilla, C., Ponomarenko, J. V., Raghava, G. P. S., van Regenmortel, M. H. V., Roggen, E. L., Sette, A., Schlessinger, A., Sollner, J., Zand, M. & Peters, B. (2007). Towards a consensus on datasets and evaluation metrics for developing B-cell epitope prediction tools. Journal of Molecular Recognition: An Interdisciplinary Journal, 20(2), 75-82. https://doi.org/10.1002/jmr.815
  11. Inouye, K., Yamano, K., Maeno, Y., Nakajima, K., Matsuoka, M., Wada, Y. & Sorimachi, M. (1992). Iridovirus infection of cultured red sea bream, Pagrus major. Fish Pathology, 27(1), 19-27. https://doi.org/10.3147/jsfp.27.19
  12. Jechlinger, W. (2006). Optimization and delivery of plasmid DNA for vaccination. Expert review of vaccines, 5(6), 803-825. https://doi.org/10.1586/14760584.5.6.803
  13. Jeong, J. B., Jun, L. J., Yoo, M. H., Kim, M. S., Komisar, J. L. & Jeong, H. D. (2003). Characterization of the DNA nucleotide sequences in the genome of red sea bream iridoviruses isolated in Korea. Aquaculture, 220(1-4), 119-133. https://doi.org/10.1016/S0044-8486(02)00538-0
  14. Jeong, M. A., Jeong, Y. J. & Kim, K. I. (2021). Complete genome sequences and pathogenicity analysis of two red sea bream iridoviruses isolated from cultured fish in Korea. Fishes, 6(4), 82. https://doi.org/10.3390/fishes6040082
  15. Jin, J. W., Nam, J. H., Kim, K. I., Hong, S. H., Byon, J. Y. & Jeong, H. D. (2011). Characterization of the Repetitive Sequences Present in the ORF25 Geno- mic Region of Megalocytiviruses from Ornamental Fishes. Korean Journal of Fisheries and Aquatic Sciences, 44(4), 352-358. https://doi.org/10.5657/KFAS.2011.0352
  16. Jung, S. J. & Oh, M. J. (2000). Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel), in southern coastal areas of the Korean peninsula. Journal of Fish Diseases, 23(3). https://doi.org/10.1046/j.1365-2761.2000.00212.x
  17. Kim, Y. J., Jung, S. J., Choi, T. J., Kim, H. R., Rajendran, K. V. & Oh, M. J. (2002). PCR amplification and sequence analysis of irido-like virus infecting fish in Korea. Journal of Fish Diseases, 25(2). https://doi.org/10.1046/j.1365-2761.2002.00345.x
  18. Kim, K. I., Hwang, S. D., Cho, M. Y., Jung, S. H., Kim, Y. C. & Jeong, H. D. (2018). A natural infection by the red sea bream iridovirus-type Megalocytivirus in the golden mandarin fish Siniperca scherzeri. Journal of fish diseases, 41(8), 1229-1233. https://doi.org/10.1111/jfd.12815
  19. Kim, K. I., Lee, E. S., Do, J. W., Hwang, S. D., Cho, M., Jung, S. H., Jee, B. Y., Kwon, W. J. & Jeong, H. D. (2019). Genetic diversity of Megalocytivirus from cultured fish in Korea. Aquaculture, 509, 16-22. https://doi.org/10.1016/j.aquaculture.2019.05.014
  20. Kim, G. H., Kim, M. J., Choi, H. J., Koo, M. J., Kim, M. J., Min, J. G. & Kim, K. I. (2021). Evaluation of a novel TaqMan probe-based real-time polymerase chain reaction (PCR) assay for detection and quantitation of red sea bream iridovirus. Fisheries and Aquatic Sciences, 24(11), 351-359. https://doi.org/10.47853/FAS.2021.e34
  21. Kim, K. H., Kang, G., Woo, W. S., Sohn, M. Y., Son, H. J., Kim, J. W., Kong, H. J., Kim, Y. O. & Park, C. I. (2024). Evaluation of the diagnostic assays detecting red sea bream iridovirus infection at different severity levels. Journal of Virological Methods, 114901. https://doi.org/10.1016/j.jviromet.2024.114901
  22. Kolaskar, A. S. & Tongaonkar, P. C. (1990). A semi-empirical method for prediction of antigenic determinants on protein antigens. FEBS letters, 276(1-2), 172-174. https://doi.org/10.1016/0014-5793(90)80535-Q
  23. Kurita, J., Nakajima, K., Hirono, I. & Aoki, T. (1998). Polymerase chain reaction (PCR) amplification of DNA of red seabream iridovirus (RSIV). Fish Pathology, 33: 17-23. https://doi.org/10.3147/jsfp.33.17
  24. Kurita, J. & Nakajima, K. (2012). Megalocytiviruses. Viruses, 4: 521-538. https://doi.org/10.3390/v4040521
  25. Kwon, W. J., Choi, J. C., Hong, S., Kim, Y. C., Jeong, M. G., Min, J. G., Jeong, J. B. & Jeong, H. D. (2020). Development of a high-dose vaccine formulation for prevention of megalocytivirus infection in rock bream (Oplegnathus fasciatus). Vaccine, 38(51), 8107-8115. https://doi.org/10.1016/j.vaccine.2020.11. 001
  26. Lee, W. L., Kim, S. R., Yun, H. M., Kitamura, S. I., Jung, S. J. & Oh, M. J. (2007). Detection of red sea bream iridovirus (RSIV) from marine fish in the Southern coastal area and East China Sea. Journal of fish pathology, 20(3), 211-220. https://www.ksfp.org/28/3262731
  27. Li, W., Joshi, M. D., Singhania, S., Ramsey, K. H. & Murthy, A. K. (2014). Peptide vaccine: progress and challenges. Vaccines, 2(3), 515-536. https://doi.org/10.3390/vaccines2030515
  28. Lin, T., Xing, J., Tang, X., Sheng, X., Chi, H. & Zhan, W. (2023). Immune and protective effects of subunit vaccines from S-domain or P-domain in capsid protein against nervous necrosis virus in pearl gentian grouper. Aquaculture, 566, 739177. https://doi.org/10.1016/j.aquaculture.2022.739177
  29. Liu, H. I., Chiou, P. P., Gong, H. Y. & Chou, H. Y. (2015). Cloning of the major capsid protein (MCP) of grouper iridovirus of Taiwan (TGIV) and preliminary evaluation of a recombinant MCP vaccine against TGIV. International Journal of Molecular Sciences, 16(12), 28647-28656. https://doi.org/10.3390/ijms161226118
  30. Musthaq, S. K. S. & Kwang, J. (2015). Reprint of "Evolution of specific immunity in shrimp-A vaccination perspective against white spot syndrome virus". Developmental & Comparative Immunology, 48(2), 342-353. https://doi.org/10.1016/j.dci.2014.07.016
  31. Oh, M. J., Kitamura, S. I., Kim, W. S., Park, M. K., Jung, S. J., Miyadai, T. & Ohtani, M. (2006). Susceptibility of marine fish species to a megalocytivirus, turbot iridovirus, isolated from turbot, Psetta maximus (L.). Journal of Fish Diseases, 29(7), 415-421. https://doi.org/10.1111/j.1365-2761.2006.0
  32. Pattanayak, S., Paul, A. & Sahoo, P. K. (2020). Detection and genetic analysis of infectious spleen and kidney necrosis virus (ISKNV) in ornamental fish from non-clinical cases: First report from India. BioRxiv, 2020-08. https://doi.org/10.1101/2020.08.12.247650
  33. Senapin, S., Dong, H. T., Meemetta, W., Gangnonngiw, W., Sangsuriya, P., Vanichviriyakit, R., Sonthi, M. & Nuangsaeng, B. (2019). Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia. Journal of fish diseases, 42(1), 119-127. https://doi.org/10. 1111/jfd.12915 https://doi.org/10.1111/jfd.12915
  34. Shinmoto, H., Taniguchi, K., Ikawa, T., Kawai, K. & Oshima, S. I. (2009). Phenotypic diversity of infectious red sea bream iridovirus isolates from cultured fish in Japan. Applied and environmental micro- biology, 75(11), 3535-3541. https://doi.org/10.1128/AEM.02255-08
  35. Shin, D. J., Jeong, Y. S., Kim, M. J., Kim, G. H. & Kim, K. I. (2023). Correlation between clinical changes and viral genome copy number in rock bream infected with red sea bream iridovirus. Journal of fish pathology, 36(2), 229-238. http://www.ksfp.org/28/12139824
  36. Shih, T. C., Ho, L. P., Wu, J. L., Chou, H. Y. & Pai, T. W. (2019). A voting mechanism-based linear epitope prediction system for the host-specific Iridoviridae family. BMC bioinformatics, 20, 49-58. https://doi.org/10.1186/s12859-019-2736-2
  37. Skwarczynski, M. & Toth, I. (2016). Peptide-based synthetic vaccines. Chemical science, 7(2), 842-854. https://doi.org/10.1039/C5SC03892H
  38. Sohn, S. G., Choi, D. L., Do, J. W., Hwang, J. Y. & Park, J. W. (2000). Mass mortalities of cultured striped beakperch, Oplegnathus fasciatus by iridoviral infection. Journal of fish pathology, 13(2), 121-127. https://www.ksfp.org/28/3057924
  39. Sommerset, I., Krossoy, B., Biering, E. & Frost, P. (2005). Vaccines for fish in aquaculture. Expert review of vaccines, 4(1), 89-101. https://doi.org/10.1586/14760584.4.1.89
  40. Thompson, J. D., Higgins, D. G. & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids research, 22(22), 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  41. Tian, H., Fu, F., Li, X., Chen, X., Wang, W., Lang, Y., Cong, F., Liu, C., Tong, G. & Li, X. (2011). Identification of the immunogenic outer membrane protein A antigen of Haemophilus parasuis by a proteomics approach and passive immunization with monoclonal antibodies in mice. Clinical and Vaccine Immunology, 18(10), 1695-1701. https://doi.org/10.1128/CVI.05223-11
  42. Xiong, X. P., Dong, C. F., Weng, S. P., Zhang, J., Zhang, Y. & He, J. G. (2011). Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus. Fish & Shellfish Immunology, 31(6), 919-924. https://doi.org/10.1016/j.fsi.2011.08.009
  43. WOAH, World Organization for Animal Health, Manual of Diagnostic Tests for Aquatic Animal. Chapter 2.3.7. Red sea bream iridoviral disease. 2021. https://www.woah.org/fileadmin/Home/eng/Health_standards/aahm/current/2.3.07_RSIVD.pdf
  44. Zhao, Z., Xiong, Y., Zhang, C., Jia, Y. J., Qiu, D. K., Wang, G. X. & Zhu, B. (2020). Optimization of the efficacy of a SWCNTs-based subunit vaccine against infectious spleen and kidney necrosis virus in mandarin fish. Fish & Shellfish Immunology, 106, 190-196. https://doi.org/10.1016/j.fsi.2020.07.062
  45. Zhang, Z., Liu, G., Liu, J., Zhu, B., Wang, G. & Ling, F. (2021). Epitope screening of the major capsid protein within grouper iridovirus of Taiwan and the immunoprotective effect with SWCNTs as the vaccine carrier. Fish & Shellfish Immunology, 117, 17-23. https://doi.org/10.1016/j.fsi.2021.07.013
  46. Zhu, B., Zhang, C., Yang, B., Guo, Z. R., Zheng, Y. Y., Gong, Y. M. & Wang, G. X. (2019). Preliminary screening and immunogenicity analysis of antigenic epitopes of spring viremia of carp virus. Fish & shellfish immunology, 94, 833-841. https://doi.org/10.1016/j.fsi.2019.09.063