Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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DNA barcoding of fish diversity from Batanghari River, Jambi, Indonesia

  • Huria Marnis (Research Center for Fishery, National Research and Innovation Agency (BRIN)) ;
  • Khairul Syahputra (Research Center for Fishery, National Research and Innovation Agency (BRIN)) ;
  • Jadmiko Darmawan (Research Center for Fishery, National Research and Innovation Agency (BRIN)) ;
  • Dwi Febrianti (Research Center for Limnology and Water Resources, National Research and Innovation Agency (BRIN)) ;
  • Evi Tahapari (Research Center for Fishery, National Research and Innovation Agency (BRIN)) ;
  • Sekar Larashati (Research Center for Limnology and Water Resources, National Research and Innovation Agency (BRIN)) ;
  • Bambang Iswanto (Research Center for Fishery, National Research and Innovation Agency (BRIN)) ;
  • Erma Primanita Hayuningtyas Primanita (Research Center for Fishery, National Research and Innovation Agency (BRIN)) ;
  • Mochamad Syaifudin (Program Study of Aquaculture, Faculty of Agriculture, Sriwijaya University) ;
  • Arsad Tirta Subangkit (Research Center for Fishery, National Research and Innovation Agency (BRIN))
  • Received : 2023.10.08
  • Accepted : 2023.11.20
  • Published : 2024.02.29
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Abstract

Global climate change, followed by an increase in anthropogenic activities in aquatic ecosystems, and species invasions, has resulted in a decline in aquatic organism biodiversity. The Batanghari River, Sumatra's longest river, is polluted by mercury-containing illegal gold mining waste (PETI), industrial pollution, and domestic waste. Several studies have provided evidence suggesting a decline in fish biodiversity within the Batanghari River. However, a comprehensive evaluation of the present status of biodiversity in this river is currently lacking. The species under investigation were identified through various molecular-based identification methods, as well as morphological identification, which involved the use of neighbor-joining (NJ) trees. All collected specimens were initially identified using morphological techniques and subsequently confirmed with molecular barcoding analysis. Morphological and DNA barcoding identification categorized all specimens (1,692) into 36 species, 30 genera and 16 families, representing five orders. A total of 36 DNA barcodes were generated from 30 genera using a 650-bp-long fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene. Based on the Kimura two-parameter model (K2P), The minimum and maximum genetic divergences based on K2P distance were 0.003 and 0.331, respectively, and the average genetic divergence within genera, families, and orders was 0.05, 0.12, 0.16 respectively. In addition, the average interspecific distance was approximately 2.17 times higher than the mean intraspecific distance. Our results showed that the COI barcode enabled accurate fish species identification in the Batanghari River. Furthermore, the present work will establish a comprehensive DNA barcode library for freshwater fishes along Batanghari River and be significantly useful in future efforts to monitor, conserve, and manage fisheries in Indonesia.

Keywords

Acknowledgement

We express our gratitude to the local governments in Jambi province for their invaluable assistance during the process of sampling and conducting environmental monitoring in the Batanghari River. We would also like to express our gratitude to Despa Surianis, Dwi Setianingsih, Ahmad Junaidi, Surya Roza, Rulif Aziz Kusnita, Riana Yulianti, and Lukman for their valuable technical assistance provided during this study.

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