한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제37권1호
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  • Pages.1-10
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  • 2024
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
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국내 갈매나무과 13종에 대한 부위별 물 추출물의 면역 증진 활성

Immune-enhancing Activity of Water Extracts for Each Part of 13 Species (Rhamnaceae) in Korea

  • 정대희 (국립산림과학원 산림약용자원연구소) ;
  • 최민영 (국립산림과학원 산림약용자원연구소) ;
  • 박광훈 (국립산림과학원 산림약용자원연구소)
  • Dae Hui Jeong (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Min Yeong Choi (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Gwang Hun Park (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 투고 : 2023.09.06
  • 심사 : 2023.11.08
  • 발행 : 2024.02.01
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초록

본 연구에서는 국내 자생하고 있는 갈매나무과 식물 13종에 대한 부위별(잎, 가지, 열매) 물 추출물의 NO 생성능을 통하여 면역증진 활성을 측정하여 기능성을 검토하였다. 망개나무, 헛개나무, 까마귀베개, 산황나무, 갈매나무, 참갈매나무, 상동나무 등에서 50% 이상 NO 생성능을 보여줌으로써 대식세포 활성화를 검증하였다. 또한 PCR 전기영동을 통하여 면역증진과 관련된 cytokine인 iNOS, COX-2, IL-1β, IL-6, TNF-α의 발현을 확인하였다. 본 연구를 통해 갈매나무과 분류군들의 부위별 NO 생성능, mRNA 발현 등과 같은 면역 활성에 대한 과학적 근거를 제시할 수 있었고, 이는 면역증진 관련 소재 발굴 및 제품 개발에 기초자료로 활용할 수 있을 것으로 판단된다.

This studied the immune-enhancing activity properties of water extracts from the leaves, branches, and fruit of 13 species (Rhamnaceae) collected during the bearing season (Berchemia berchemiifolia, B. floribunda, Hovenia dulcis, Paliurus ramosissimus, Rhamnella franguloides, Rhamnus crenata, R. davurica, R. koraiensis, R. parvifolia, R. ussuriensis, R. yoshinoi, Sageretia thea, and Ziziphus jujube). Immune-enhancing activity were studied using the nitric oxide (NO) production in RAW264.7 cells. Extracts of B. berchemiifolia, H. dulcis, R. franguloides, R. crenata, R. davurica, R. ussuriensis and S. thea showed strong immune-enhancing activity through NO production. In addition, the expression of immune enhancement-related cytokine genes (NOS, COX-2, IL-1β, IL-6 and TNF-α) were confirmed through PCR-electrophoresis. The results of this study suggest that Rhamnaceae extracts can be used as natural antioxidants and immune enhancer.

키워드

과제정보

본 연구는 2020년도 국립산림과학원 일반연구과제 '갈매나무과 식물의 항염증 물질 탐색 및 약리기전 연구(과제번호: FP0400-2019-01)' 의해 이루어진 것으로 이에 감사드립니다.

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