한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제37권1호
  • /
  • Pages.11-21
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  • 2024
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
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육계(Cortex of Cinnamomum cassia J.Presl) 추출물로부터 부정근 형성 유도물질 분리 및 동정

Isolation and Identification of Adventitious Root Formation Inducing Substances from Cortex of cinnamomum cassia J.Presl

  • 여주호 (국립안동대학교 생약자원학과) ;
  • 백정규 (부부한의원) ;
  • 박지성 ((주)한국식물환경연구소) ;
  • 김건우 (국립안동대학교 생약자원학과)
  • Joo Ho Yeo (Department of Medicinal Plant Resources, Andong National University) ;
  • Jeong Kyu Baek (Bubu Oriental Medical Clinic) ;
  • Jee Sung Park (Korea Plants Environment Research) ;
  • Kun Woo Kim (Department of Medicinal Plant Resources, Andong National University)
  • 투고 : 2023.09.17
  • 심사 : 2023.11.07
  • 발행 : 2024.02.01
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초록

본 연구에서 육계 추출물로부터 cinnamyl alcohol을 분리·정제하였다. 연구 결과, H3 분획100 ㎍/mL에서 부정근 형성율이 286.6%였으며, 표준품 cinnamyl alcohol 1 mM (134.2 ㎍/mL)의 형성율이 290%로 유사한 결과를 나타내었다. 따라서 육계 methanol 추출물에 함유되어있는 cinnamyl alcohol이 녹두의 부정근 형성을 유도하는 주된 화합물일 것으로 판단된다. 육계에서 동정한 cinnamyl alcohol은 현재 식품첨가제 및 향료로써 사용되고 있으므로 안전성이 이미 보장되어 있으며, 한약재로써 사용된 육계 잔류물을 활용하여 부정근 형성 유도물질을 추출한다면비용적인 측면에서 또한 긍정적인 요인으로 작용할 것으로 판단된다. 따라서 육계는 유기농 농자재로써 충분히 사용 가능할 뿐만 아니라 신규 천연 식물생장조절제의 개발용 소재로서도 활용 가능할 것으로 사료된다.

In this study, as a result of exploring the physiological activity of plants useful for agriculture on various plant resources, it was possible to confirm an activity similar to auxin that promotes plant rooting in methanol extract of Cinnamon Bark (cortex of Cinnamomum cassia J.Presl). After separating the active body by applying column chromatography and HPLC to the CHCl3 active fraction obtained by solvent extraction for each polarity from the methanol extract of cinnamon bark, cinnamyl alcohol was identified through GC/MS analysis. By bioassay using cinnamyl alcohol standard and the active fraction separated and purified from the methanol extract of cinnamon bark, the rooting rate of mung bean seedlings of the cinnamyl alcohol standard was 290% compared with the untreated control at 134.2 ㎍/mL concentration, and the adventitious root formation activity similar to the rooting rate (268.6%; 100 ㎍/mL) of the active fraction was shown. In conclusion, it is believed that cinnamyl alcohol contained in methanol extract of Cinnamon Bark is the main compound that induces adventitious root formation in mung bean.

키워드

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