International Journal of Industrial Entomology and Biomaterials

  • 제48권1호
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  • Pages.13-18
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  • 2024
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
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Inhibitory effects of Oxya chinensis sinuosa ethanol extract on RANKL-induced osteoclast differentiation

  • Ra-Yeong Choi (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Bong Sun Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Sohyun Park (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Minchul Seo (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Joon Ha Lee (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • HaeYong Kweon (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • In-Woo Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
  • 투고 : 2023.10.26
  • 심사 : 2023.12.17
  • 발행 : 2024.03.31
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초록

The rice field grasshopper, Oxya chinensis sinuosa (OC), has traditionally been utilized in Korea for various purposes; however, its potential benefits in the context of osteoporosis remain unclear. The results revealed that OC ethanol extract (OCE) significantly inhibited the formation and activity of tartrate-resistant acid phosphatase (TRAP)-positive cells in receptor activator of nuclear factor-κB ligand (RANKL)-stimulated RAW264.7 cells. Furthermore, OCE, at concentrations ranging from 100 to 400 ㎍/mL, demonstrated a dose-dependent reduction in the protein expression of osteoclast-specific markers, including nuclear factor of activated T cell cytoplasmic 1, c-Src, and TRAP, when compared to RANKL stimulation alone. Additionally, OCE significantly inhibited RANKL-induced activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) but not the activation of extracellular signal-regulated kinase. Collectively, these results indicate that OCE suppresses osteoclastogenesis by attenuating the phosphorylation of p38 MAPK and JNK. Consequently, these findings suggest that OCE holds promise for the prevention of osteoporosis.

키워드

과제정보

This work was supported by the Rural Development Administration, Republic of Korea (Project No. PJ01563203).

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