Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Salmonella vector induces protective immunity against Lawsonia and Salmonella in murine model using prokaryotic expression system

  • Sungwoo Park (College of Veterinary Medicine, Jeonbuk National University, Iksan Campus) ;
  • Eunseok Cho (Swine Science Division, National Institute of Animal Science) ;
  • Amal Senevirathne (College of Veterinary Medicine, Jeonbuk National University, Iksan Campus) ;
  • Hak-Jae Chung (Swine Science Division, National Institute of Animal Science) ;
  • Seungmin Ha (Dairy Science Division, National Institute of Animal Science) ;
  • Chae-Hyun Kim (Swine Science Division, National Institute of Animal Science) ;
  • Seogjin Kang (Dairy Science Division, National Institute of Animal Science) ;
  • John Hwa Lee (College of Veterinary Medicine, Jeonbuk National University, Iksan Campus)
  • Received : 2023.08.27
  • Accepted : 2023.11.24
  • Published : 2024.01.31
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Abstract

Background: Lawsonia intracellularis is the causative agent of proliferative enteropathy and is associated with several outbreaks, causing substantial economic loss to the porcine industry. Objectives: In this study, we focused on demonstrating the protective effect in the mouse model through the immunological bases of two vaccine strains against porcine proliferative enteritis. Methods: We used live-attenuated Salmonella Typhimurium (ST) secreting two selected immunogenic LI antigens (Lawsonia autotransporter A epitopes and flagellin [FliC]-peptidoglycan-associated lipoprotein-FliC) as the vaccine carrier. The constructs were cloned into a Salmonella expression vector (pJHL65) and transformed into the ST strain (JOL912). The expression of immunogenic proteins within Salmonella was evaluated via immunoblotting. Results: Immunizing BALB/c mice orally and subcutaneously induced high levels of LI-specific systemic immunoglobulin G and mucosal secretory immunoglobulin A. In immunized mice, there was significant upregulation of interferon-γ and interleukin-4 cytokine mRNA and an increase in the subpopulations of cluster of differentiation (CD) 4+ and CD 8+ T lymphocytes upon splenocytes re-stimulation with LI antigens. We observed significant protection in C57BL/6 mice against challenge with 106.9 times the median tissue culture infectious dose of LI or 2 × 109 colony-forming units of the virulent ST strain. Immunizing mice with either individual vaccine strains or co-mixture inhibited bacterial proliferation, with a marked reduction in the percentage of mice shedding Lawsonia in their feces. Conclusions: Salmonella-mediated LI gene delivery induces robust humoral and cellular immune reactions, leading to significant protection against LI and salmonellosis.

Keywords

Acknowledgement

We thank Editage (www.editage.com/ac) for editing a draft of this manuscript.

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