현장농수산연구지 (Journal of Practical Agriculture & Fisheries Research)

국립한국농수산대학교 교육개발센터 (Korea National University of Agriculture and Fisheries)
권호 표지
DOI QR코드

DOI QR Code

국내산 양식 흰다리새우 유래의 비브리오균에 대한 소독제의 방제 효과 검증

Assessment of the Effect of a Disinfectant to Control Vibrio Strain from White Leg Shrimps Cultured in Korea

  • 전진우 (국립한국농수산대학교 수산양식전공)
  • Jin Woo Jun (Department of Aquaculture, Korea National University of Agriculture and Fisheries)
  • 투고 : 2023.09.18
  • 심사 : 2023.10.13
  • 발행 : 2023.10.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

2023년 7월, 국내의 흰다리 새우 양식장에서 양식 중이던 흰다리 새우가 이상유영을 보이며 식욕부진에 시달리며 지속적으로 폐사하였다. 초기에는 일일 평균 폐사율이 0.1%였으나, 3주 후에는 5%까지 증가하였다. 질병 진단 과정 중, 폐사 새우의 간췌장에서 세균(KNUAFVa-SHP08147)이 분리되었다. 본 세균의 16S rRNA 유전자의 시퀀스 분석결과, 분리주 KNUAFVa-SHP08147은 Vibrio alginolyticus로 동정이 되었다. 본 연구에서는, 분리주를 대상으로 비브리오 방제 효과가 있다고 알려져 있는 소독제의 효능을 검증해보았다. 소독제 VirkonTMS의 효능 검증을 위하여, VirkonTMS를 처리한 후 KNUAFVa-SHP08147의 성장 곡선을 조사하였다. 실험 결과, VirkonTMS가 KNUAFVa-SHP08147의 성장에 미치는 영향이 무의미한 것으로 드러나, 소독제 VirkonTMS의 Vibrio 방제 효과는 없는 것으로 드러났다.

White leg shrimps which were cultured in a private aquaculture farm showed abnormal swimming behavior and appetite reduction in July 2023. Then, gradual mortality was observed in the aquaculture farm. During the diagnosis, bacterial strain KNUAFVaSHP08147 was isolated from the hepatopancreas of the dead shrimps. Based on the sequence of 16S rRNA gene, KNUAFVa-SHP08147 was proved to be Vibrio alginolyticus, showing 99.71 % nucleotide identity with that of V. alginolyticus MG54 and GS MYPK1. According to the result of the growth of KNUAFVa-SHP08147, the treatment a commercial disinfectant, VirkonTMS was not proved to be effective to prohibit the growth of Vibrio.

키워드

참고문헌

  1. Benzie, J.A.H. (2009). Use and exchange of genetic resources of penaeid shrimps for food and aquaculture. Rev Aquacult 1: 232-250.  https://doi.org/10.1111/j.1753-5131.2009.01018.x
  2. De Silva BCJ, Hossain S, Wimalasena SHMP, Pathirana HNKS, Heo GJ. (2018). Putative virulence traits and antibiogram profile of Aeromonas spp. isolated from frozen white-leg shrimp (Litopenaeus vannamei) marketed in Korea. J Food Saf 38: e12470. 
  3. Hall TA. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95-98. 
  4. Han JE, Mohney LL, Tang KFJ, Pantoja CR, Lightner DV. (2015). Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps. Aquac Rep 2: 17-21.  https://doi.org/10.1016/j.aqrep.2015.04.003
  5. Jun JW, Han JE, Tang KFJ, Lightner DV, Kim J, Seo SW, Park SC. (2016). Potential application of bacteriophage pVp-1: Agent combating Vibrio parahaemolyticus strains associated with acute hepatopancreatic necrosis disease (AHPND) in shrimp. Aquaculture 457: 100-103.  https://doi.org/10.1016/j.aquaculture.2016.02.018
  6. Jun JW, Han JE, Giri SS, Tang KFJ, Zhou X, Aranguren LF, Kim HJ, Yun S, Chi C, Kim SG, Park SC. (2017). Phage application for the protection from acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei. Indian J Microbiol 58: 114-117. 
  7. Larkin MA, Blackshields G, Brown NP, et al. (2007). Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948.  https://doi.org/10.1093/bioinformatics/btm404
  8. Lightner DV. (1983). Diseases of cultured penaeid shrimp. CRC Handbook of Mariculture Vol. 1: 289-320. 
  9. Lightner DV. (1988). Vibrio disease of penaeid shrimp. Dev Aquac Fish Sci (Netherlands). 
  10. Lightne DV, Redman RM, Pantoja CR, Noble BL, Tran LH. (2012). Early mortality syndrome affects shrimp in Asia. Glob Aqua Advocate 15: 40. 
  11. Tamura K, Stecher G, Kumar S. (2021). MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38: 3022-3027.  https://doi.org/10.1093/molbev/msab120
  12. Thompson FL, Iida T, Swings J. (2004). Biodiversity of Vibrios. Microbiol Mol Biol Rev 68: 403-431.  https://doi.org/10.1128/MMBR.68.3.403-431.2004
  13. Wyban J. (2009). World shrimp farming revolution: industry impact of domestication, breeding and widespread use of specific pathogen free Penaeus vannamei. Proceedings of the special session on sustainable shrimp farming. World Aquaculture 2009: 12-21.