Journal of Microbiology and Biotechnology

  • 제33권8호
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  • Pages.1023-1029
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  • 2023
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Production of a New Biosurfactant by a New Yeast Species Isolated from Prunus mume Sieb. et Zucc.

  • Jeong-Seon Kim (Agricultural Microbiology Division. National Institute of Agricultural Science, Rural Development Administration) ;
  • Miran Lee (Agricultural Microbiology Division. National Institute of Agricultural Science, Rural Development Administration) ;
  • Dae-Won Ki (Division of Biotechnology and Advanced institute of Environmental and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University) ;
  • Soon-Wo Kwon (Agricultural Microbiology Division. National Institute of Agricultural Science, Rural Development Administration) ;
  • Young-Joon Ko (Agricultural Microbiology Division. National Institute of Agricultural Science, Rural Development Administration) ;
  • Jong-Shik Kim (Marine industry research institute for east sea rim) ;
  • Bong-Sik Yun (Division of Biotechnology and Advanced institute of Environmental and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University) ;
  • Soo-Jin Kim (Agricultural Microbiology Division. National Institute of Agricultural Science, Rural Development Administration)
  • 투고 : 2022.05.27
  • 심사 : 2023.04.21
  • 발행 : 2023.08.28
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초록

Biosurfactants reduce surface and interfacial tension due to their amphiphilic properties and are an eco-friendly alternative for chemical surfactants. In this study, a new yeast strain JAF-11 that produces a biosurfactant was selected using drop collapse method, and the properties of the extracts were investigated. The nucleotide sequences of the strain were compared with closely related strains and identified based on the D1/D2 domain of the large subunit ribosomal DNA (LSU) and internal transcribed spacer (ITS) regions. Neodothiora populina CPC 39399T, the closest species with strain JAF-11, showed a sequence similarity of 97.75% for LSU and 94.27% for ITS, respectively. The result suggests that the strain JAF-11 represents a distinct species that cannot be assigned to any existing genus or species in the family Dothideaceae. Strain JAF-11 produced a biosurfactant reducing the surface tension of water from 72 mN/m to 34.5 mN/m on the sixth day of culture and the result of measuring the critical micelle concentration (CMC) by extracting the crude biosurfactant was found to be 24 mg/l. The molecular weight 502 of the purified biosurfactant was confirmed by measuring the fast atom bombardment mass spectrum. The chemical structure was analyzed by measuring 1H nuclear magnetic resonance (NMR), 13C NMR, and two-dimensional NMRs of the compound. The molecular formula was C26H46O9, and it was composed of one octanoyl group and two hexanoyl groups to myo-inositol moiety. The new biosurfactant is the first report of a compound produced by a new yeast strain, JAF-11.

키워드

과제정보

This work was supported by a grant National Institute of Agricultural Science, Rural Development Administration (Project No. PJ01567501, PJ01354906) in Korea. The authors thank Ms. Ji-Young Oh, Center for University-wide Research Facilities (CURF) at Jeonbuk National University, for performing NMR measurements.

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