Journal of Microbiology and Biotechnology

  • 제33권8호
  • /
  • Pages.1013-1022
  • /
  • 2023
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Combined Application Effects of Arbuscular Mycorrhizal Fungi and Biochar on the Rhizosphere Fungal Community of Allium fistulosum L.

  • Chunxiang Ji (Schools of Life Sciences, Anhui Agricultural University) ;
  • Yingyue Li (Schools of Life Sciences, Anhui Agricultural University) ;
  • Qingchen Xiao (Schools of Life Sciences, Anhui Agricultural University) ;
  • Zishan Li (Schools of Life Sciences, Anhui Agricultural University) ;
  • Boyan Wang (Schools of Life Sciences, Anhui Agricultural University) ;
  • Xiaowan Geng (Schools of Life Sciences, Anhui Agricultural University) ;
  • Keqing Lin (Schools of Life Sciences, Anhui Agricultural University) ;
  • Qing Zhang (Schools of Life Sciences, Anhui Agricultural University) ;
  • Yuan Jin (Schools of Life Sciences, Anhui Agricultural University) ;
  • Yuqian Zhai (Schools of Life Sciences, Anhui Agricultural University) ;
  • Xiaoyu Li (Schools of Life Sciences, Anhui Agricultural University) ;
  • Jin Chen (Schools of Life Sciences, Anhui Agricultural University)
  • 투고 : 2023.03.20
  • 심사 : 2023.06.07
  • 발행 : 2023.08.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Arbuscular mycorrhizal fungi (AMF) are widespread soil endophytic fungi, forming mutualistic relationships with the vast majority of land plants. Biochar (BC) has been reported to improve soil fertility and promote plant growth. However, limited studies are available concerning the combined effects of AMF and BC on soil community structure and plant growth. In this work, a pot experiment was designed to investigate the effects of AMF and BC on the rhizosphere microbial community of Allium fistulosum L. Using Illumina high-throughput sequencing, we showed that inoculation of AMF and BC had a significant impact on soil microbial community composition, diversity, and versatility. Increases were observed in both plant growth (the plant height by 8.6%, shoot fresh weight by 12.1%) and root morphological traits (average diameter by 20.5%). The phylogenetic tree also showed differences in the fungal community composition in A. fistulosum. In addition, Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that 16 biomarkers were detected in the control (CK) and AMF treatment, while only 3 were detected in the AMF + BC treatment. Molecular ecological network analysis showed that the AMF + BC treatment group had a more complex network of fungal communities, as evidenced by higher average connectivity. The functional composition spectrum showed significant differences in the functional distribution of soil microbial communities among different fungal genera. The structural equation model (SEM) confirmed that AMF could improve the microbial multifunctionality by regulating the rhizosphere fungal diversity and soil properties. Our findings provide new information on the effects of AMF and biochar on plants and soil microbial communities.

키워드

과제정보

This research was supported by the Key Research and Development Program of Anhui Province (202204c06020021) and the Natural Science Foundation of China (U21A20235 and 32201308).

참고문헌

  1. Schimmelpfennig S, Glaser B. 2012. One step forward toward characterization: some important material properties to distinguish biochars. J. Environ. Qual. 41: 1001-1013. https://doi.org/10.2134/jeq2011.0146
  2. Ajayi AE, Horn R. 2017. Biochar-induced changes in soil resilience: effects of soil texture and biochar dosage. Pedosphere 27: 236-247. https://doi.org/10.1016/S1002-0160(17)60313-8
  3. Laird D, Fleming P, Wang B, Horton R, Karlen D. 2010. Biochar impact on nutrient leaching from a midwestern agricultural soil. Geoderma 158: 436-442. https://doi.org/10.1016/j.geoderma.2010.05.012
  4. Ippolito JA, Laird DA, Busscher WJ. 2012. Environmental benefits of biochar. J. Environ. Qual. 41: 967-972. https://doi.org/10.2134/jeq2012.0151
  5. Fiaz K, Danish S, Younis U, Malik SA, Raza Shah MH, Niaz S. 2014. Drought impact on Pb/Cd toxicity remediated by biochar in Brassica campestris. J. Soil Sci. Plant Nutr. 14: 845-854. https://doi.org/10.4067/S0718-95162014005000067
  6. Ok YS, Chang SX, Gao B, Chung HJ. 2015. SMART biochar technology-a shifting paradigm towards advanced materials and healthcare research. Environ. Technol. Innov. 4: 206-209. https://doi.org/10.1016/j.eti.2015.08.003
  7. Jabborova D, Wirth S, Kannepalli A, Narimanov A, Desouky S, Davranov K, et al. 2020. Coinoculation of rhizobacteria and biochar application improves growth and nutrients in soybean and enriches soil nutrients and enzymes. Agronomy 10: 1142.
  8. Brundrett MC, Tedersoo L. 2018. Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytol. 220: 1108-1115. https://doi.org/10.1111/nph.14976
  9. Baum C, El-Tohamy W, Gruda N. 2015. Increasing the productivity and product quality of vegetable crops using AMF: a review. Sci. Hortic. 187: 131-141. https://doi.org/10.1016/j.scienta.2015.03.002
  10. Begum N, Qin C, Ahanger MA, Raza S, Khan MI, Ashraf M, et al. 2019. Role of AMF in plant growth regulation: implications in abiotic stress tolerance. Front. Plant Sci. 10: 1068.
  11. Cavagnaro TR, Jackson LE, Six J, Ferris H, Goyal S, Asami D. 2006. Arbuscular mycorrhizas, microbial communities, nutrient availability, and soil aggregates in organic tomato production. Plant Soil 282: 209-225. https://doi.org/10.1007/s11104-005-5847-7
  12. Nunes JLD, de Souza PVD, Marodin GAB, Fachinello JC. 2010. Effect of AMF and indole butyric acid interaction on vegetative growth of 'Aldrighi' peach rootstock seedlings. Cienc. Agrotecnol. 34: 80-86. https://doi.org/10.1590/S1413-70542010000100010
  13. Wu QS, Zou YN, Liu W, Ye XF, Zai HF, Zhao LJ. 2010. Alleviation of salt stress in citrus seedlings inoculated with mycorrhiza: changes in leaf antioxidant defense systems. Plant Soil Environ. 56: 470-475. https://doi.org/10.17221/54/2010-PSE
  14. Ravnskov S, Larsen J. 2016. Functional compatibility in cucumber mycorrhizas in terms of plant growth performance and foliar nutrient composition. Plant Biol. 18: 816-823. https://doi.org/10.1111/plb.12465
  15. Kumar A, Sharma S, Mishra S, Dames JF. 2013. Arbuscular mycorrhizal inoculation improves growth and antioxidative response of Jatropha curcas (L.) under Na2SO4 salt stress. Plant Biosyst. 149: 260-269. https://doi.org/10.1080/11263504.2013.845268
  16. Lehmann J, Rillig MC, Thies J, Masiello CA, Hockaday WC, Crowley D. 2011. Biochar effects on soil biota-a review. Soil Biol. Biochem. 43: 1812-1836. https://doi.org/10.1016/j.soilbio.2011.04.022
  17. Guo CX, Pan ZY, Peng SA. 2016. Effect of biochar on the growth of Poncirus trifoliata (L.) Raf. seedlings in Gannan acidic red soil. Soil Sci. Plant Nut. 62: 194-200. https://doi.org/10.1080/00380768.2016.1150789
  18. Ohsowski BM, Dunfield K, Klironomos JN, Hart MM. 2018. Plant response to biochar, compost, and mycorrhizal fungal amendments in post-mine sandpits. Restor. Ecol. 26: 63-72. https://doi.org/10.1111/rec.12528
  19. Liu L, Li JW, Yue FX, Yan XW, Wang FY, Bloszies S, et al. 2018. Effects of arbuscular mycorrhizal inoculation and biochar amendment on maize growth, cadmium uptake and soil cadmium speciation in Cd-contaminated soil. Chemosphere 194: 495-503. https://doi.org/10.1016/j.chemosphere.2017.12.025
  20. Liu C, Liu F, Ravnskov S, Rubaek G, Sun Z, Andersen MN. 2017. Impact of wood biochar and its interactions with mycorrhizal fungi, phosphorus fertilization and irrigation strategies on potato growth. J. Agron. Crop Sci. 203: 131-145. https://doi.org/10.1111/jac.12185
  21. Malicka M, Magurno F, Posta K, Chmura D, Piotrowska-Seget Z. 2021. Differences in the effects of single and mixed species of AMF on the growth and oxidative stress defense in Lolium perenne exposed to hydrocarbons. Ecotoxicol. Environ. Saf. 217: 112252.
  22. Maseko ST, Dakora FD. 2013. Rhizosphere acid and alkaline phosphatase activity as a marker of P nutrition in nodulated Cyclopia and Aspalathus species in the Cape fynbos of South Africa. South Afr. J. Bot. 89: 289-295. https://doi.org/10.1016/j.sajb.2013.06.023
  23. Li X, Miao W, Gong C, Jiang H, Ma W, Zhu S. 2013. Effects of prometryn and acetochlor on arbuscular mycorrhizal fungi and symbiotic system. Lett. Appl. Microbiol. 57: 122-128. https://doi.org/10.1111/lam.12084
  24. Chen WJ, Zhan YB, Zhang XJ, Shi X, Wang ZG, Xu SQ, et al. 2022. Influence of carbon-to-phosphorus ratios on phosphorus fractions transformation and bacterial community succession in phosphorus-enriched composting. Bioresour. Technol. 362: 127786.
  25. Bolyen E, Rideout JR, Dillon MR, Bokulich NA, Abnet CC, Al-Ghalith GA, et al. 2019. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat. Biotechnol 37: 852-857. https://doi.org/10.1038/s41587-019-0209-9
  26. Chen XM, Du Z, Liu D, Wang LQ, Pan CN, Wei ZM, et al. 2022. Biochar mitigates the biotoxicity of heavy metals in livestock manure during composting. Biochar 4: 48.
  27. Chen J, Li ZS, Xu DL, Xiao QC, Liu HJ, Li XY, et al. 2023. Patterns and drivers of microbiome in different rock surface soil under the volcanic extreme environment. iMeta 2: 122.
  28. Chen J, Nan J, Xu D, Mo L, Zheng Y, Chao L, et al. 2020a. Response differences between soil fungal and bacterial communities under opencast coal mining disturbance conditions. Catena 194: 104779.
  29. Chen J, Xu DL, Chao LM, Liu HJ, Bao Y. 2020b. Microbial assemblages associated with the rhizosphere and endosphere of an herbage, Leymus chinensis. Microb. Biotechnol. 13: 1390-1402. https://doi.org/10.1111/1751-7915.13558
  30. Chen J, Zheng YX, Guo YQ, Li FS, Xu DL, Chao LM, et al. 2021a. Differences in microbial communities from Quaternary volcanic soils at different stages of development: evidence from late pleistocene and holocene volcanoes. Catena 201: 105211.
  31. Mei YL, Xu J, Zhang Y, Li B, Fan SS, Xu HC. 2021. Effect of Fe-N modification on the properties of biochars and their adsorption behavior on tetracycline removal from aqueous solution. Bioresour. Technol. 325: 124732.
  32. Biederman LA, Harpole WS. 2013. Biochar and its effects on plant productivity and nutrient cycling: a meta-analysis. GCB Bioener. 5: 202-214. https://doi.org/10.1111/gcbb.12037
  33. Xu CY, Hosseini-Bai S, Hao Y, Rachaputi RC, Wang H, Xu Z, et al. 2015. Effect of biochar amendment on yield and photosynthesis of peanut on two types of soils. Environ. Sci. Pollut. Res. Int. 22: 6112-6125. https://doi.org/10.1007/s11356-014-3820-9
  34. Paetsch L, Mueller CW, Kogel-Knabner I, von Lutzow M, Girardin C, Rumpel C. 2018. Effect of in-situ aged and fresh biochar on soil hydraulic conditions and microbial C use under drought conditions. Sci. Rep. 8: 6852.
  35. Zhao ZJ, Chen L, Xiao Y. 2021. The combined use of arbuscular mycorrhizal fungi, biochar and nitrogen fertilizer is most beneficial to cultivate Cichorium intybus L. in Cd-contaminated soil. Ecotoxicol. Environ. Saf. 217: 112154.
  36. Mehari ZH, Elad Y, Rav-David D, Graber ER, Harel YM. 2015. Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling. Plant Soil. 395: 31-44. https://doi.org/10.1007/s11104-015-2445-1
  37. Abd_Allah EF, Hashem A, Alqarawi AA, Bahkali AH, Alwhibi MS. 2015. Enhancing growth performance and systemic acquired resistance of medicinal plant Sesbania sesban (L.) Merr using AMF under salt stress. Saudi J. Biol. Sci. 22: 274-283. https://doi.org/10.1016/j.sjbs.2015.03.004
  38. Hashem A, Abd Allah EF, Alqarawi AA, Al-Huqail AA, Shah MA. 2016. Induction of osmoregulation and modulation of salt Stress in Acacia gerrardii Benth. by AMF and Bacillus subtilis (BERA 71). BioMed Res. Int. 2016: 6294098
  39. Hashem A, Kumar A, Al-Dbass AM, Alqarawi AA, Al-Arjani ABF, Singh G, et al. 2019. AMF and biochar improves drought tolerance in chickpea. Saudi J. Biol. Sci. 26: 614-624. https://doi.org/10.1016/j.sjbs.2018.11.005
  40. Delgado-Baquerizo M, Maestre FT, Reich PB, Jeffries TC, Gaitan JJ, Encinar D, et al. 2016. Microbial diversity drives multifunctionality in terrestrial ecosystems. Nat. Commun. 7: 10541.
  41. Delgado-Baquerizo M, Trivedi P, Trivedi C, Eldridge DJ, Reich PB, Jeffries TC, et al. 2017. Microbial richness and composition independently drive soil multifunctionality. Funct. Ecol. 31: 2330-2343 https://doi.org/10.1111/1365-2435.12924
  42. Zhu JJ, Niu WQ, Zhan ZH, Siddique KHM, Sun D, Yang RY. 2022. Distinct roles for soil bacterial and fungal communities associated with the availability of carbon and phosphorus under aerated drip irrigation. Agric. Water Manag. 274: 107925.
  43. Zhou Y, Bastida F, Zhou B, Sun Y, Gu T, Li S, et al. 2020. Soil fertility and crop production are fostered by micro-nano bubble irrigation with associated changes in soil bacterial community. Soil Biol. Biochem. 141: 107663.
  44. Liao MZ, Miao YB, Zhang LC, Wang Y, Feng RN, Yang L, et al. 2014. System analysis of LWDH related genes based on text mining in biological networks. BioMed Res. Int. 2014: 484926.
  45. Chen J, Guo YQ, Li FS, Zheng YX, Xu DL, Liu HJ, et al. 2020c. Exploring the effects of volcanic eruption disturbances on the soil microbial communities in the montane meadow steppe. Environ. Pollut. 267: 115600.
  46. Chen J, Xu DL, Zheng YX, Chao LM, Liu HJ, Qu HT, et al. 2021b. Distinct effects of volcanic cone types on soil microbiomes: evidence from cinder cone and spatter cone. Catena 200: 105180.
  47. Huang BT, Zhang L, Cao YP, Yang YR, Wang P, Li ZX, et al. 2023. Effects of land-use type on soil organic carbon and carbon pool management index through arbuscular mycorrhizal fungi pathways. Glob. Ecol. Conserv. 43: e02432.