The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Microbiological Characteristics, Enzyme Activity, and Physiological Functionality of Unrecorded Wild Yeasts from the Wolsung Valley of South Deogyu Mountain and Jinjamcheon in Daejeon, Korea

남덕유산 월성계곡과 대전광역시 진잠천주변 등으로부터 선발된 국내 미기록 야생효모들의 균학적 특성과 주요 효소활성 및 생리활성

  • Jeong-Su Moon (Department of Biomedicine and Biotechnology, Paichai University) ;
  • Min-Young An (Department of Biomedicine and Biotechnology, Paichai University) ;
  • Mal-Geum Park (Department of Biomedicine and Biotechnology, Paichai University) ;
  • Jong-Soo Lee (Department of Biomedicine and Biotechnology, Paichai University)
  • 문정수 (배재대학교 의생명과학과) ;
  • 안민영 (배재대학교 의생명과학과) ;
  • 박맑음 (배재대학교 의생명과학과) ;
  • 이종수 (배재대학교 의생명과학과)
  • Received : 2023.02.09
  • Accepted : 2023.03.29
  • Published : 2023.03.31
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Abstract

This study aimed to screen and investigate the microbiological characteristics, enzyme activity, and physiological functionality of unrecorded wild yeasts from the Wolsung valley of South Deogyu mountain and Jinjamcheon in Daejeon city, Korea. Candida sorboxylosa WSC25-4 (NNIBRFG47336), Arxula adeninivorans WSC6-3 (NNIBRFG47335), Farysizyma taiwaniana JRC6-2 (NNIBRFG47339), Saturnispora dispora JTS19-1 (NNIBRFG47334), Vanderwaltozyma polyspora JJCH5-3 (NNIBRFG47337), and Vanrija fragicola SW-9 (NNIBRFG47338) have not been previously recorded. None of these formed spores or pseudo-mycelium, and four strains, including C. sorboxylosa WSC25-4, were global in shaped. Except for V. polyspora JJCH5-3, all strains grew well in the yeast extract-peptone-dextrose (YPD), potato-dextrose and yeast malt extract media. A. adeninivorans WSC6-3 and S. dispora JTS19-1 grew well in 40% glucose-containing YPD medium and V. fragicola SW-9 in 5% NaCl-containing YPD medium. The supernatant of A. adeninivorans WSC6-3, F. taiwaniana JRC6-2, and V. fragicola SW-9 exhibited 58.0, 59.0, and 59.0% of glucoamylase activity, respectively. C. sorboxylosa WSC25-4 also produced extracellular alkaline protease. Antioxidant activity of the supernatant from F. taiwanian JRC6-2 was high (79.8%), while the other five unrecorded yeasts demonstrated elevated antiaging superoxide dismutase (SOD)-like activity (99%). Anti-gout xanthin oxidase inhibitory activity of S. dispora JTS19-1 and V. fragicola SW-9 was also elevated at 79 and 80%, respectively.

남덕유산 월성계곡과 대전광역시 진참천 등지로부터 분리한 야생효모들 중 국내 미기록 야생효모들을선별한후, 이들의균학적특성과효소활성및생리활성등을조사하였다. 이들야생효모들중Candida sorboxylosa WSC25-4 (NNIBRFG47336), Arxula adeninivorans WSC6-3 (NNIBRFG47335), Farysizyma taiwaniana JRC6-2 (NNIBRFG47339), Saturnispora dispora JTS19-1 (NNIBRFG47334), Vanderwaltozyma polyspora JJCH5-3 (NNIBRFG47337)과Vanrija fragicola SW-9 (NNIBRFG47338) 등 6균주들을 국내 미기록 야생효모들로 최종 선별하였다. 이들 균주들은 대부분 구형으로 출아에 의해 영양증식을 하였고, 모두 자낭포자와 의균사들을 형성하지 않았다. V. polyspora JJCH5-3외 5균주들이 YPD, YM, PD등의 배지에서 잘 생육하였고, A. adeninivorans WSC6-3와S. dispora JTS19-1 균주들은 40% 포도당을함유한 YPD 배지에서잘생육하는내당성을보였으며, V. fragicola SW-9 균주는 5% NaCl을 함유한 YPD배지에서 생육하는 호염성 효모로 확인되었다. 국내 미기록 야생효모들의 배양 상등액들 중A. adeninivorans WSC6-3과F. taiwaniana JRC6-2, V. fragicola SW-9 등의 glucoamylase 활성이 각각 58.0, 59.0, 59.0% 로 비교적 높았고, C. sorboxylosa WSC25-4 균주는 알칼리성 단백질분해효소 활성이 높아 세재 산업에 유용할 것으로 사료된다. 또한, 미기록 효모들의 모든배양상등액은항산화활성과 SOD-유사활성이 70-98%로매우높았고, S. dispora JTS19-1과V. fragicola SW-9의항통풍성잔틴산화효소저해활성도각각 70%와 80%로높았다.

Keywords

Acknowledgement

This work was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea.

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