Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immobilization of Diatom Phaeodactylum tricornutum with Filamentous Fungi and Its Kinetics

  • Tyler J. Barzee (Department of Biosystems and Agricultural Engineering, University of Kentucky) ;
  • Hamed M. El-Mashad (Department of Biological and Agricultural Engineering, University of California Davis) ;
  • Andrew R. Burch (Department of Chemistry, University of California) ;
  • Annaliese K. Franz (Department of Chemistry, University of California) ;
  • Ruihong Zhang (Department of Biological and Agricultural Engineering, University of California Davis)
  • Received : 2022.09.27
  • Accepted : 2022.11.15
  • Published : 2023.02.28
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Abstract

Immobilizing microalgae cells in a hyphal matrix can simplify harvest while producing novel mycoalgae products with potential food, feed, biomaterial, and renewable energy applications; however, limited quantitative information to describe the process and its applicability under various conditions leads to difficulties in comparing across studies and scaling-up. Here, we demonstrate the immobilization of both active and heat-deactivated marine diatom Phaeodactylum tricornutum (UTEX 466) using different loadings of fungal pellets (Aspergillus sp.) and model the process through kinetics and equilibrium models. Active P. tricornutum cells were not required for the fungal-assisted immobilization process and the fungal isolate was able to immobilize more than its original mass of microalgae. The Freundlich isotherm model adequately described the equilibrium immobilization characteristics and indicated increased normalized algae immobilization (g algae removed/g fungi loaded) under low fungal pellet loadings. The kinetics of algae immobilization by the fungal pellets were found to be adequately modeled using both a pseudo-second order model and a model previously developed for fungal-assisted algae immobilization. These results provide new insights into the behavior and potential applications of fungal-assisted algae immobilization.

Keywords

Acknowledgement

Support for this work was provided by the California Energy Commission (ARV-15-008). The authors extend their gratitude to Dr. Zhiliang Fan and Dr. Bryan Jenkins for their review and thoughtful comments on an early version of the manuscript, and Sam Hornstein and Lin Cao for their assistance with lab experiments.

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