Journal of Microbiology and Biotechnology

  • 제33권2호
  • /
  • Pages.242-250
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  • 2023
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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CGI-58 Protein Acts as a Positive Regulator of Triacylglycerol Accumulation in Phaeodactylum tricornutum

  • Qin Shu (Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences) ;
  • Yufang Pan (Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences) ;
  • Hanhua Hu (Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences)
  • 투고 : 2022.09.20
  • 심사 : 2022.11.28
  • 발행 : 2023.02.28
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초록

Comparative gene identification-58 (CGI-58) is an activating protein of triacylglycerol (TAG) lipase. It has a variety of catalytic activities whereby it may play different roles in diverse organisms. In this study, a homolog of CGI-58 in Phaeodactylum tricornutum (PtCGI-58) was identified. PtCGI-58 was localized in mitochondria by GFP fusion protein analysis, which is different from the reported subcellular localization of CGI-58 in animals and plants. Respectively, PtCGI-58 overexpression resulted in increased neutral lipid content and TAG accumulation by 42-46% and 21-32%. Likewise, it also increased the relative content of eicosapentaenoic acid (EPA), and in particular, the EPA content in TAGs almost doubled. Transcript levels of genes involved in de novo fatty acid synthesis and mitochondrial β-oxidation were significantly upregulated in PtCGI-58 overexpression strains compared with wild-type cells. Our findings suggest that PtCGI-58 may mediate the breakdown of lipids in mitochondria and the recycling of acyl chains derived from mitochondrial β-oxidation into TAG biosynthesis. Moreover, this study potentially illuminates new functions for CGI-58 in lipid homeostasis and provides a strategy to enrich EPA in algal TAGs.

키워드

과제정보

This work was supported by the National Natural Science Foundation of China (41976119 and 91751117) and the International Partnership Program of Chinese Academy of Sciences (Grant No. 075GJHZ2022014MI).

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