Journal of Plant Biotechnology

  • Volume 50
  • /
  • Pages.255-266
  • /
  • 2023
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Physiological and morpho-anatomical analyses of hyperhydric Arabidopsis thaliana influenced by media components

  • Nurashikin Kemat (Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia) ;
  • Richard G.F. Visser (Plant Breeding, Wageningen University and Research) ;
  • Frans A. Krens (Plant Breeding, Wageningen University and Research)
  • Received : 2023.11.05
  • Accepted : 2023.12.11
  • Published : 2023.12.19
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Abstract

Hyperhydricity is a physiological anomaly that significantly affects the growth and proliferation rate of crops cultivated by tissue culture techniques. To better understand the mechanisms that govern hyperhydricity incidence, we examined the effects of several media components, particularly cytokinin and gelling agents. These elements were found to be influential in both in vitro propagation and the development of hyperhydricity. Our study revealed that Arabidopsis thaliana seedlings had a greater manifestation of hyperhydricity symptoms when exposed to high cytokinin concentrations compared with the control. The presence of gelrite led to the manifestation of hyperhydric symptoms by elevated water build-up in the apoplast. The phenomenon of stomata closure was observed in the hyperhydric leaves, resulting in an increased ability to retain water and a decrease in the transpiration rates when compared to their respective control leaves. Additionally, histological examinations of the cross sections of hyperhydric leaves revealed an irregular cellular arrangement and large intercellular spaces. Furthermore, hyperhydric seedlings displayed impaired cuticular development in comparison to their normal seedlings.

Keywords

Acknowledgement

We would like to express our gratitude to Dr. G.J.M (Geert-Jan) de Klerk for his valuable and constructive recommendations during the design and development stages of this research project. We also want to express our heartfelt gratitude to Luo Rong and Ziqi Zeng for their dedicated efforts and valuable contributions on this research topic. This manuscript is a reworked version of chapter 2 and 6 from the first authors PhD thesis (Kemat 2020). This research was funded in part by the Dutch Ministry of Economic Affairs, TKI-TU grant KV1310-067 and by Ministry of Higher Education Malaysia and Universiti Putra Malaysia.

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