Journal of Plant Biotechnology

  • Volume 50
  • /
  • Pages.267-274
  • /
  • 2023
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of dark incubation in germination of indirect date palm somatic embryos and conversion into plantlets

  • Mansour Abohatem (Department of Biology, Faculty of Applied Sciences, Amran University) ;
  • Yousra Al-Qubati (Plant Tissue Culture Laboratory, Public Corporation for Agricultural Services, Ministry of Agriculture and Irrigation) ;
  • Hanan Abohatem (Plant Tissue Culture Laboratory, Public Corporation for Agricultural Services, Ministry of Agriculture and Irrigation)
  • Received : 2023.09.03
  • Accepted : 2023.10.23
  • Published : 2023.12.20
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Abstract

All studies on date palm somatic embryogenesis have focused on germination in the presence of light while neglecting germination in darkness, which mimics the germination process of zygotic embryos within seeds. To improve the date palm micropropagation protocol, we investigated the effects of light and darkness incubation on the germination of indirect date palm somatic embryos and their subsequent conversion into plantlets. Darkness incubation emerged as a pivotal factor in the germination of indirect date palm somatic embryos and their successful conversion into plantlets. Darkness incubation significantly decreased the time required for the conversion of indirect somatic embryos into plantlets, halving the duration from 24 weeks to only 12 weeks. The micropropagation protocol was modified, consolidating the previous two distinct stages of germination and elongation under light incubation into a single stage under darkness incubation. These findings modified the protocol and significantly reduced the overall duration of the date palm micropropagation protocol.

Keywords

References

  1. Abohatem M, Baaziz M (2015) In vitro date palm somatic embryo from cell suspension culture. Lambert Academic Publishing, Berlin
  2. Abohatem M, Zouine J, El Hadrami I (2011) Low concentrations of BAP and high rate of subcultures improve the establishment and multiplication of somatic embryos in date palm suspension cultures by limiting oxidative browning associated with high levels of total phenols and peroxidase activities. Sci Hort 130:344-348. https://doi.org/10.1016/j.scienta.2011.06.045
  3. Abohatem MA, Bakil Y, Baaziz M (2017) Plant regeneration from somatic embryogenic suspension cultures of date palm. In: Al-Khayri J, Jain S, Johnson D (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_17
  4. Abohatem, M, Abohatem H, Al-Qubati Y (2020) Effect of light conditions on germination and conversion of indirect date palm somatic embryos into plants. In: Beltane Bolduc (eds) Date Palm: Composition, Cultivation and Uses. Nova Sci Pub 229-242
  5. Aderkas P, Teyssier C, Charpentier JP, Gutmann M, Paques L, Le Mette C, Ader K, Label P, Kong L, Lelu-Walter MA (2015) Effect of light conditions on anatomical and biochemical aspects of somatic and zygotic embryos of hybrid larch (Larix marschlinsii). Ann Bot. 115(4):605-615. https://doi.org/10.1093/aob/mcu254
  6. Alabadi D, Gil J, Blazquez MA, Garcia-Martinez JL (2004) Gibberellins repress photomorphogenesis in darkness. Plant Physiol 134(3):1050-7. DOI: 10.1104/pp.103.035451
  7. Al-Khalifah N, Shanavaskhan AE (2012) Micropropagation of date palms. Asia-Pacific Consortium on Agricultural Biotechnology (APCoAB) and Association of Agricultural Research Institutions in the Near East and North Africa (AARINENA). p 54
  8. Al-Khalifah N, Askari E, Shanavaskhan AE (2013) Date palm tissue culture and genetical identification of cultivars grown in Saudi Arabia. Riyadh, KSA
  9. Al-Khayri JM, Naik PM, Jain SM, Johnson DV (2018) Advances in date palm (Phoenix dactylifera L.) breeding. In Advances in Plant Breeding Strategies: Fruits. Springer. pp 727-771
  10. AL-Mayahi AM (2016) Effect of red and blue light emitting diodes "CRB-LED" on in vitro organogenesis of date palm (Phoenix dactylifera L.) cv. Alshakr. World J of Microbiol and Biotech 32:160. https://doi.org/10.1007/s11274-016-2120-6
  11. Almusawi A, Alshnawa A, Alkhalifa A, Almesfer R (2015) Induction of direct somatic embryogenesis in date palm (Phoenix dactylifera L.) cv. Hellawi. AAB Bio f lux 7(2):80-89. http://www.aab.bioflux.com.ro/docs/2015.80-89.pdf
  12. Ba-Angood S (2015). Date Palm Status and Perspective in Yemen. In: Al-Khayri J, Jain S, Johnson D (eds) Date Palm Genetic Resources and Utilization. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9707-8_7
  13. Bekheet SA, Saker MM, Taha HS, Moursy HA (2001) Plant regeneration via somatic embryogenesis in date palm (Phoenix dactylifera L.). Arab J Biotechnol 4:111-118
  14. Bradford MM (1956) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  15. Cheong EJ, Pooler MR (2004) Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink. Plant Cell Rep 22:810-815. https://doi.org/10.1007/s00299-004-0771-5
  16. El Hadrami I, baaziz M (1995) Somatic embryogenesis and analysis of peroxidase in Phoenix dactylifera L. Bio Plant 37:197-203. https://doi.org/10.1007/BF02913210
  17. Elhiti M, Stasolla C, Wang A (2013) Molecular regulation of plant somatic embryogenesis. In Vitro Cell Dev Biol - Plant 49:631-642. https://doi.org/10.1007/s11627-013-9547-3
  18. Feher A (2015) Somatic embryogenesis-stress-induced remodeling of plant cell fate. Biochim Biophys Acta 1849:385-402. https://doi.org/10.1016/j.bbagrm.2014.07.005
  19. Ferreira W, Suzuki RM, Pescador R, Rita de Cassia L, Figueiredo-Ribeiro, Kerbauy GB (2011) Propagation, growth, and carbohydrates of Dendrobium Second Love (Orchidaceae) in vitro as affected by sucrose, light, and dark. In Vitro Cell Dev Biol-Plant 47:420-427. https://doi.org/10.1007/s11627-010-9311-x
  20. Fiore MC, Trabace T, Sunseri F (1997) High frequency of plant regeneration in sunflower from cotyledons via somatic embryogenesis. Plant Cell Rep 16:295-298. https://doi.org/10.1007/BF01088284
  21. Fki L, Kriaa W, Nasri A, Baklouti E, Chkir O, Masmoudi RB, Rival A, Drira N (2017) Indirect somatic embryogenesis of date palm using juvenile leaf explants and low 2,4-D concentration. In: Al-Khayri J, Jain S, Johnson D (eds) Date Palm Biotechnology Protocols Volume I. Meth. in Mol Biol Vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_9
  22. George EF (1993) Plant Propagation by Tissue Culture Part 1. England: Exegetics Ltd, England
  23. Griffiths J, Halliday K (2011) Plant development: Light exposure directs meristem fate. Current Biology 21(19):817-819. https://doi.org/10.1016/j.cub.2011.08.054
  24. Grzyb M, Kalandyk A, Waligorski P, Mikula A (2017) The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb. Plant Cell Tiss Organ Cult 129:387-397. https://doi.org/10.1007/s11240-017-1185-8
  25. Jain SM (2012) Date palm biotechnology: Current status and prospective-an overview. Emir J Food Agric 24:386-399
  26. Kvaalen H, Appelgren M (1999) Light quality influences germination, root growth and hypocotyl elongation in somatic embryos but not in seedlings of Norway spruce. In vitro Cell Dev Biol Plant 35:437-441. https://doi.org/10.1007/s11627-999-0064-3
  27. Lecouteux CG, Lai FM, bryan D, Mc Kresieb D (1993) Maturation of Alfalfa (Medicago setiva L.) somatic embryos by abscisic acid, sucrose and chilling stress. Plant sci 94:207-213. https://doi.org/10.1016/0168-9452(93)90021-Q
  28. Liu ZH, Liu HY, Wang HY (1996) Effect of light on indole-3-acetic acid in soybean hypocotyls. Bot Bull Acad Sin 37:113-119
  29. Macheix JJ, Fleuriet A, Billot J (1990) Fruit Phenolics. CRC Press, Inc., Boca Raton, Florida, p 378
  30. May RA, Trigiano RN (1991) Somatic embryogenesis and plant regeneration from leaves of Dendranthema grandiflora. J Am Soc Hortic Sci 116:366-371. https://doi.org/10.21273/JASHS.116.2.366
  31. Mazri MA, Meziani R (2015) Micropropagation of date palm: A Review. Cell Dev Biol 4:160
  32. Mazri MA, Naciri R, Belkoura I (2020) Maturation and conversion of somatic embryos derived from seeds of olive (Olea europaea L.) cv. Dahbia: occurrence of secondary embryogenesis and adventitious bud formation. Plants 9:1489
  33. Meziani R, Jaiti F, Mazri MA, Anjarn M, Chitt MA, El Fadile J, Alem C (2015) Effects of plant growth regulators and light intensity on the micropropagation of date palm (Phoenix dactylifera L) cv. Mejhoul. J of Crop Sci and Biotech 18:325-331. https://doi.org/10.1007/s12892-015-0062-4
  34. Mikula A, Pozoga M, Grzyb M, Rybczyn'ski (2015) An unique system of somatic embryogenesis in the tree fern Cyathea delgadii Sternb.: the importance of explant type, and physical and chemical factors. Plant Cell Tiss Organ Cult 123:467-478. https://doi.org/10.1007/s11240-015-0850-z
  35. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physio Plantarum 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  36. Pacholczak A, Szydlo W, Lukaszewska A (2005) The effect of etiolation and shading of stock plants on rhizogenesis in stem cuttings of Cotinus coggygria . Acta Physiologiae Plantarum 27(4A):417-428. https://doi.org/10.1007/s11738-005-0046-y
  37. Ree JF, Guerra MP (2015) Palm (Arecaceae) somatic embryogenesis. In Vitro Cell Dev Biol-Plant 51(6):589-602. https://doi.org/10.1007/s11627-015-9722-9
  38. Shehata WF, Belal AFH, El-Deeb MD (2015) Effect of light Intensity and Temperature on in vitro Somatic Embryos Germination Stage in Date Palm. Arab Gulf J Sci Res 33(2/3):82-89 https://doi.org/10.51758/AGJSR-2/3-2015-0008
  39. Soundar Raju, C, Aslam A, Kathiravan K, Palani P, Shajahan A (2014) Direct somatic embryogenesis and plant regeneration from leaf sheath explants of mango ginger (Curcuma amada Roxb.). In Vitro Cell Dev Biol-Plant 50:752-759. https://doi.org/10.1007/s11627-014-9653-x
  40. SPSS (1996) 7.0 for Windows 95. SPSS Inc., Chicago
  41. Sunandar A, Doly, Supena JDE (2017) Inducation of somatic embryogenesis in Sengon (Fallcataria molaccana) with Thidaizuron and light treatments. HAYATI J of Bios 24:105-108. https://doi.org/10.1016/j.hjb.2017.08.002
  42. Suzuki RM, Kerbauy GB (1999) Efeito da giberelina sobre o crescimento de plantas de Catasetum fimbriatum (Orchidaceae), incubadas na presenca e ausencia de luz. Braz J of Plant Physiol 11:172-173
  43. Suzuki RM, Kerbauy GB (2006) Effects of light and ethylene on endogenous hormones and development of Catasetum fimbriatum (Orchidaceae). Braz J Plant Physiol 18(3):359-365. https://doi.org/10.1590/S1677-04202006000300002
  44. Suzuki RM, Kerbauy GB, Pescador, R, Purgatto E, Ceccantini G, Ferreira W (2010) Dark- induced hormone changes coincide with the resumption of light-inhibited shoot growth in Catasetum fimbriatum (Orchidaceae). J of Plant Physiology 167:375-381. https://doi.org/10.1016/j.jplph.2009.10.002
  45. Suzuki, RM, Kerbauy GB, Zaffari GR (2004) Endogenous hormonal levels and growth of dark- incubated shoots of Catasetum fimbriatum (Morren) Lendl. J Plant Physiol 161:929-935 https://doi.org/10.1016/j.jplph.2003.11.001
  46. Symons GM, Reid JB (2003) Hormone levels and response during de-etiolation in pea. Planta 216:422-431. https://www.jstor.org/stable/23387339
  47. Taha RA (2017) Enhanced indirect somatic embryogenesis of date palm using low levels of seawater. In: Date Palm Biotechnology Protocols Volume I., edited by Al-Khayri J, Jain S, Johnson D. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_6
  48. Takamura T, Tanaka M (1996) Somatic embryogenesis from the etiolated petiole of cyclamen (Cyclamen persicum Mill.). Plant Tissue Cult Lett 13:43-48 https://doi.org/10.5511/plantbiotechnology1984.13.43
  49. Zayed ZE (2017) Enhanced indirect somatic embryogenesis from shoot-tip explants of date palm by gradual reductions of 2,4-D concentration. In: Al-Khayri J, Jain S, Johnson D (eds) Date Palm Biotechnology Protocols Volume I. Meth in Mol Biol, Vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_7
  50. Zobayet SMA, Saxena PK (2003) In vitro regeneration of Echinacea purpurea L: enhancement of somatic embryogenesis by indole butyric acid and dark preincubation. In Vitro Cell Dev Biol Plant 39:605-12. https://doi.org/10.1079/IVP2003434
  51. Zouine J, El Hadrami I (2007) Effect of 2,4-D, glutamine and BAP on embryogenic suspension culture of date palm (Phoenix dactylifera L.). Sci Hort 112:221-226. https://doi.org/10.1016/j.scienta.2006.12.041