Journal of Applied Biological Chemistry

  • Volume 66
  • /
  • Pages.23-28
  • /
  • 2023
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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The immune enhancement effect of Nelumbo nucifera Gaertner Seed Extract (NSE) in murine macrophage RAW 264.7 cells

RAW 264.7 대식세포에서 연자육 추출물(Nelumbo nucifera Gaertner Seed Extract, NSE)의 면역 증강 효과

  • Se Jeong Kim (School of Food Science and Biotechnology, Kyungpook National University) ;
  • San Kim (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Se Hyeon Jang (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Sung Ran Yoon (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Bo Ram So (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Jeong Min Park (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Jung A Ryu (ivision of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Sung Keun Jung (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2022.12.06
  • Accepted : 2023.01.19
  • Published : 2023.12.31
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Abstract

Since the global shock caused by COVID-19, interest in immune-enhancing materials is rapidly increasing, therefore, the development of novel materials is necessary from the industrial and health perspectives. In this study, we selected Nelumbo nucifera Gaertner Seed Extract (NSE) and evaluated immune enhancement effect by using RAW 264.7 murine macrophage cells. NSE significantly up-regulated production of nitric oxide and reactive oxygen species without affecting cell viability in RAW 264.7 cells. Additionally, NSE exhibited an increase of inducible nitric oxide synthase and cyclooxygenase-2 expression in RAW 264.7 cells. The enzyme-linked immunosorbent assay results showed that NSE-treatment significantly enhanced production of interleukin 6 and tumor necrosis factor-α in RAW 264.7 cells. Furthermore, we observed that NSE significantly up-regulated phosphorylation of p65, I kappa B kinase α/β, and I kappa B (IκB) α as well as down-regulation of IκB α expression in RAW 264.7 cells. Our findings indicate that NSE could be the potential health-functional food material with capacity of improving immunity via Nuclear factor-kappa B signaling pathway.

코로나바이러스-19로 인한 세계적인 펜데믹 이후 면역력 강화소재에 대한 관심이 급격히 증가하고 있으므로 산업적, 건강적 측면에서 새로운 소재의 개발이 필요하다. 본 연구에서는 연자육 추출물을 소재로 선정하고 RAW 264.7 쥐 대식세포를 이용하여 면역증진 효과를 평가하였다. 연자육 추출물은 RAW 264.7 세포에서 세포 생존력에 독성을 나타내지 않으면서 nitric oxide 및 reactive oxygen species의 생산을 상향 조절하였다. 또한 연자육 추출물은 RAW 264.7 세포에서 inducible nitric oxide synthase 및 cyclooxygenase-2 발현을 크게 증가시켰다. Enzyme-linked immunosorbent assay 결과에서는 연자육 추출물의 처리가 RAW 264.7 세포에서 interleukin 6 및 tumor necrosis factor-α의 생성을 유의미하게 향상시키는 것으로 나타났다. 또한 연자육 추출물이 p65, I kappa B kinase α/β, 및 I kappa B (IκB) α의 인산화를 크게 상향 조절하고, RAW 264.7 세포에서 IκB α의 발현을 하향 조절하였다. 우리의 연구 결과는 연자육 추출물이 Nuclear factor-kappa B 신호전달 경로를 통해 면역력을 향상시킬 수 있는 잠재적인 건강기능식품 소재가 될 수 있음을 나타낸다.

Keywords

Acknowledgement

본 연구는 2022년 경상북도기술원의 1팀 1교수 책임제 공동연구 '경북식재료활용K-면역농식품기술개발' (과제번호: LP0048882022) 연구비 지원에 의하여 수행되었습니다.

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