Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Neuroprotective Effect of Gardeniae Fructus against Oxidative Damage Induced by tert-Butyl Hydroperoxide in PC12 Cells

PC12 cell에서 tert-butyl hydroperoxide로 유도된 산화적 손상에 대한 치자의 신경보호효과

  • Jong Rok, Lee (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Sang Chan, Kim (College of Korean Medicine, Daegu Haany University) ;
  • Sung Hui, Byun (College of Korean Medicine, Daegu Haany University) ;
  • Sook Jahr, Park (Department of Pharmaceutical Engineering, Daegu Haany University)
  • 이종록 (대구한의대학교 제약공학과) ;
  • 김상찬 (대구한의대학교 한의과대학(방제학 교실)) ;
  • 변성희 (대구한의대학교 한의과대학(방제학 교실)) ;
  • 박숙자 (대구한의대학교 제약공학과)
  • Received : 2023.02.08
  • Accepted : 2023.02.17
  • Published : 2023.02.28
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Abstract

Objective : Gardeniae Fructus (GF) is the ripe fruit of Gardenia jasminoides Ellisa with a bitter taste and cold properties. Ingredient compounds including geniposide are known to have anti-inflammatory, antioxidant, and neuroprotective effects. The purpose of this study was to investigate the neuroprotective effect of GF on tBHP-induced PC12 cells. Methods : Cell viability was measured by the MTT assay, and apoptosis was determined by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The expression level of each protein was monitored by Western blot analysis, and reactive oxygen species (ROS) were analyzed using DCFH-DA. Results : In PC12 cells, tBHP induced cell death through apoptosis with caspase activation and PARP inactivation. Cells treated with tBHP showed an increase in intracellular ROS and depletion of GSH. Pretreatment with GF prevented tBHP-induced apoptosis, reduced ROS, and increased GSH. GF also maintained increased Nrf2 expression in the presence of tBHP. Phosphorylation of JNK and p38 MAPK was increased by tBHP, whereas phosphorylation of ERK was decreased. GF restored changes in ERK and p38 phosphorylation, but not JNK phosphorylation. Conclusion : These results indicate that GF has neuroprotective effects through anti-apoptotic and antioxidant effects mediated by regulation of Nrf2 expression and phosphorylation of ERK and p38. It also demonstrates the potential use of GF as a source of antioxidant and neuroprotective substances.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MOE) (No. 2017R1D1A3B03031863).

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