대한본초학회지 (The Korea Journal of Herbology)

  • 제38권1호
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  • Pages.31-43
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  • 2023
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
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알코올성 간 손상 동물 모델에서 芍藥 葛根 복합물의 간 손상 보호 효과

Protective effects of Paeoniae Radix Alba and Puerariae Radix combination on alcoholic liver disease

  • 최정원 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김진영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 박해진 (대구한의대학교 DHU 바이오융복합시험센터)
  • Jeong Won, Choi (Department of Herbology, Korean Medicine of College, Daegu Haany University) ;
  • Jin Young, Kim (Department of Herbology, Korean Medicine of College, Daegu Haany University) ;
  • Mi-Rae, Shin (Department of Herbology, Korean Medicine of College, Daegu Haany University) ;
  • Hae-Jin, Park (DHU Bio Convergence Testing Center)
  • 투고 : 2022.12.15
  • 심사 : 2023.01.25
  • 발행 : 2023.01.30
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초록

Objective : Alcoholic liver disease (ALD) is caused by excess alcohol intake. In the liver, alcohol breakdown results formation of toxic byproducts that lead to damage to tissue. This study is to investigate the therapeutic effects of Paeoniae Radix Alba and Puerariae Radix combination (PP) on ALD. Methods : PP was analyzed for polyphenolic compounds and free radical scavenging activity. ALD mouse model was induced by feeding ethanol and water (Control), silymarin (50 mg/kg), low-dose (PP: 100 mg/kg) or high-dose (PP: 200 mg/kg) was orally administrated to ALD mice for 14 days. The serum was assessed with levels of AST, ALT, total bilirubin, total cholesterol, and triglyceride. Liver tissues were evaluated for ROS levels, degree of liver damage and protein expression. Results : The 3:1 (Paeoniae Radix Alba:Puerariae Radix) ratio showed the best antioxidant values for the experiment. In ALD model, levels of AST, ALT, total bilirubin, total cholesterol, and triglyceride were significantly increased in the Control and the levels were decreased by treatment of PP. In addition, increased ROS, ONOO- and MDA levels in the Control were reduced in the PP groups. Western blot analysis figured out that proteins related to ROS and cholesterol metabolism were higher in ALD than in PP-treated ALD. Antioxidant enzyme expression was low in the control group and increased by PP treatment. Conclusion : Our results suggest that PP has the potential to be a medicine in ALD in terms of regulating oxidative stress and adjusting lipid metabolism.

키워드

과제정보

본 연구는 산업통상자원부와 한국산업기술진흥원의 "사회적경제혁신성장사업" (과제번호 P0017625)과, 2022년도 정부재원 (과학기술정보통신부 여성과학기술인 R&D 경력복귀 지원사업)으로 한국여성과학기술인육성재단의 지원 (협약번호 제 2022-339호)을 받아 연구되었습니다.

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