The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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First Report of Charcoal Rot Caused by Macrophomina phaseolina on Peanut Plants in Korea

땅콩에서 Macrophomina phaseolina에 의한 균핵마름병 발생 보고

  • Soo Yeon Choi (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • You Kyoung Lee (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Chang Ok Geum (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Shinhwa Kim (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Hyunjung Chung (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Sang-Min Kim (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Yong Hoon Lee (Division of Biotechnology, Jeonbuk National University)
  • 최수연 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 이유경 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 금창옥 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김신화 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 정현정 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김상민 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 이용훈 (전북대학교 생명공학부)
  • Received : 2023.12.07
  • Accepted : 2023.12.20
  • Published : 2023.12.31
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Abstract

Peanut plants showing mild wilt were found in fields of Iksan, Korea, in August 2021. The diseased peanut plants were collected, and the causal pathogens were isolated using potato dextrose agar (PDA) medium. The isolated IS-1 strain formed white mycelia on PDA, which turned black with age. Sclerotia were produced on the PDA and barley leaves laid on water agar 7 d after incubation at 30℃. The sequences of both the internal transcribed spacer (ITS) region and calmodulin gene of IS-1 showed a 100% similarity with that of Macrophomina phaseolina. A phylogenetic tree constructed using the ITS regions of fungal pathogens causing disease in peanut plants indicated that the IS-1 stain belongs to M. phaseolina. The inoculation of IS-1 sclerotia into peanut seedlings resulted in yellowing and wilt symptoms in aboveground plants and brown to dark rots in roots 35-40 d after inoculation. Overall, the morphological characteristics, molecular identification, and pathogenicity of IS-1 indicate that the causal pathogen is M. phaseolina. This is the first report of charcoal rot caused by M. phaseolina on peanut plants in Korea. Further study is needed to develop the control measures for charcoal rot in peanut plants.

Keywords

Acknowledgement

This work was supported by "Cooperative Research Program for Agriculture Science & Technology Development project (PJ016095022023)" Rural Development Administration, Korea.

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