Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Distribution of the extended-spectrum beta-lactamase genes derived from microorganisms in the waterfront environments

주변 수계에서 미생물유래 extended-spectrum beta-lactamase 유전자의 분포

  • Young-Min Bae (Department of Life Science and Public Health, Changwon University)
  • 배영민 (창원대학교 생명보건학부)
  • Received : 2022.11.30
  • Accepted : 2022.12.29
  • Published : 2022.12.30
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Abstract

Water samples were collected from three spots(Namcheon, Changwoncheon and Cheongwoonji) in Changwon and genomic DNA was isolated from them. Quantitative PCR was performed with the isolated DNA as template and primers targeting five different class A extended-spectrum beta-lactamase(ESBL) genes(blaOXA-1, blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9). The number of total ESBL genes from each sample showed large variations between each sample. Thirty nanograms of DNA from Namcheon contained 1.93×106 copies of ESBL genes whereas the same amount of DNA from Changwoncheon contained 1.47×105 copies of ESBL genes. However, the same amount of DNA from Cheongwoonji pond contained only 9.5×103 copies of ESBL genes. The ratio of each ESBL genes showed little difference between Namcheon river and Changwoncheon river, but DNA from Cheongwoonji pond showed a large difference from the rest. blaOXA-1 gene was present at 65.3%, and blaCTX-M-1 gene 33.6% for Namcheon comprising together almost 99%. blaOXA-1 gene was present at 64.1%, and blaCTX-M-1 gene 19.1% for Changwoncheon comprising together over 83%. blaCTX-M-1 gene was present at 87.5% and blaCTX-M-9 genes 9.8% for Cheongwoonji, a pond which is a closed and isolated environment.

창원시의 3개 지점(남천, 창원천, 청운지)에서 물 시료를 채취하고, 채취한 시료로부터 genomic DNA를 분리하였다. 분리된 DNA와 class A extended-spectrum beta-lactamase(ESBL) 유전자 5가지(blaOXA-1, blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9)를 target으로 하는 primers를 사용하여 quantitative PCR을 수행하였다. 시료를 채취한 지점별로 ESBL 유전자들의 수와 비율에서 확연한 차이가 있음을 알 수 있었다. 남천의 경우 DNA 30ng당 ESBL 유전자가 1.93×106 copy나 존재하는 데에 반해 창원천의 경우에는 1.47×105 copy 그리고 청운지에는 9.5×103 copy 밖에 존재하지 않았다. 흐르는 하천인 남천과 창원천에서는 각 ESBL 유전자들의 비율에서 큰 차이가 없었다. 즉, 남천에서는 blaOXA-1 유전자가 65.3%, blaCTX-M-1 유전자가 33.6%를 차지하여서 이 두 유전자가 전체 ESBL 유전자의 99% 가까이를 차지하고 있었다. 창원천에서도 blaOXA-1 유전자가 64.1%, blaCTX-M-1 유전자가 19.1%를 차지하므로 이 두 유전자가 전체의 83% 이상을 차지하고 있었다. 하천과 다르게 폐쇄된 환경인 청운지 연못에서는 내성세균들의 총량 자체가 다른 두 하천에 비해서 월등하게 적었다. 또한 내성 세균의 비율도 달라서 blaCTX-M-1 유전자가 전체의 87.5%, 그리고 blaCTX-M-9 유전자가 9.8%를 차지하고 있었다.

Keywords

Acknowledgement

이 논문은 2021~2022년도 창원대학교 자율연구과제 연구비 지원으로 수행된 연구결과임.

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