Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Age quadratically affects intestinal calcium and phosphorus transporter gene expression in broiler chickens

  • Lv, Xianliang (College of Animal Science and Technology, Henan Agricultural University) ;
  • Hao, Junfang (Department of Animal Science, College of Biology and Food, Shangqiu Normal University) ;
  • Wu, Lihua (College of Animal Science and Technology, Henan Agricultural University) ;
  • Liu, Mengyuan (College of Animal Science and Technology, Henan Agricultural University) ;
  • He, Lei (Department of Animal Science, College of Biology and Food, Shangqiu Normal University) ;
  • Qiao, Yingying (Department of Biochemistry and Biotechnology, Sumy National Agrarian University) ;
  • Cui, Yanyan (Department of Animal Science, College of Biology and Food, Shangqiu Normal University) ;
  • Wang, Guan (Department of Animal Science, College of Biology and Food, Shangqiu Normal University) ;
  • Zhang, Chunmei (Department of Animal Science, College of Biology and Food, Shangqiu Normal University) ;
  • Qu, Hongxia (Department of Animal Science, College of Biology and Food, Shangqiu Normal University) ;
  • Han, Jincheng (Department of Animal Science, College of Biology and Food, Shangqiu Normal University)
  • Received : 2022.02.16
  • Accepted : 2022.04.28
  • Published : 2022.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: This research aimed to evaluate the effects of age on growth, tibia development, and intestinal calcium (Ca) and phosphorus (P) transporter gene expressions in broiler chickens. Methods: A total of 224 male Arbor Acres broilers were fed with nutrient-adequate diets and reared in eight cages (28 broilers per cage). Eight broilers (one broiler per cage) were selected and killed at 5, 10, 15, 20, 25, 30, 35, and 40 days of age, respectively. Results: Body weight continuously increased with age of broiler chickens from 5 to 40 days. The bone weight, ash weight, diameter, and length of the tibia also increased with broiler age. By contrast, the tibia ash, Ca, and P percentages quadratically changed with age (p<0.001), and the highest values of mineral contents were observed at 20, 25, and 25 days of age, respectively. The mRNA abundances of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), and plasma membrane ATPase 1b (PMCA1b) increased from 5 to 25 days and then decreased up to 40 days. Similar results were noted in the mRNA abundances of IIb sodium-phosphate cotransporter (NaPi-IIb), inorganic phosphate transporter 1 (PiT-1), inorganic phosphate transporter 2 (PiT-2), nuclear vitamin D receptor (nVDR), and membrane vitamin D receptor (mVDR). The mRNA abundances of Ca and P transporters and VDRs were the highest at 25 days of age. Conclusion: These data indicate that age quadratically affects intestinal Ca and P transporter gene expression and mineral absorption capacity in broiler chickens.

Keywords

Acknowledgement

This work was supported by the National Natural Science Foundation of China (32072753).

References

  1. Proszkowiec-Weglarz M, Schreier LL, Miska KB, Angel R, Kahl S, Russell B. Effect of early neonatal development and delayed feeding post-hatch on jejunal and ileal calcium and phosphorus transporter genes expression in broiler chickens. Poult Sci 2019;98:1861-71. https://doi.org/10.3382/ps/pey546
  2. Han JC, Wang XN, Wu LH, et al. Dietary calcium levels regulate calcium transporter gene expression levels in the small intestine of broiler chickens. Br Poult Sci 2022;63:202-10. https://doi.org/10.1080/00071668.2021.1949697
  3. Proszkowiec-Weglarz M, Angel R. Calcium and phosphorus metabolism in broilers: effect of homeostatic mechanism on calcium and phosphorus digestibility. J Appl Poult Res 2013; 22:609-27. https://doi.org/10.3382/japr.2012-00743
  4. Li P, Wang R, Jiao H, Wang X, Zhao J, Lin H. Effects of dietary phosphorus level on the expression of calcium and phosphorus transporters in laying hens. Front Physiol 2018;9:627. https://doi.org/10.3389/fphys.2018.00627
  5. Villa-Bellosta R, Sorribas V. Role of rat sodium/phosphate cotransporters in the cell membrane transport of arsenate. Toxicol Appl Pharmacol 2008;232:125-34. https://doi.org/10.1016/j.taap.2008.05.026
  6. Giral H, Caldas Y, Sutherland E, et al. Regulation of rat intestinal Na-dependent phosphate transporters by dietary phosphate. Am J Physiol Renal Physiol 2009;297:1466-75. https://doi.org/10.1152/ajprenal.00279.2009
  7. Shao YX, Wen Q, Zhang SM, et al. Dietary supplemental vitamin D3 enhances phosphorus absorption and utilisation by regulating gene expression of related phosphate transporters in the small intestine of broilers. Br J Nutr 2019;121:9-21. https://doi.org/10.1017/S0007114518002763
  8. Nemere I, Farach-Carson MC, Rohe B, et al. Ribozyme knockdown functionally links a 1,25(OH)2D3 membrane binding protein (1,25D3-MARRS) and phosphate uptake in intestinal cells. Proc Natl Acad Sci USA 2004;101:7392-7. https://doi.org/10.1073/pnas.0402207101
  9. Wood RJ, Fleet JC, Cashman K, Bruns ME, Deluca HF. Intestinal calcium absorption in the aged rat: evidence of intestinal resistance to 1,25(OH)2 vitamin D. Endocrinology 1998;139:3843-8. https://doi.org/10.1210/endo.139.9.6176
  10. Armbrecht HJ, Boltz MA, Christakos S, Bruns MEH. Capacity of 1,25-dihydroxyvitamin D to stimulate expression of calbindin D changes with age in the rat. Arch Biochem Biophys 1998; 352:159-64. https://doi.org/10.1006/abbi.1998.0594
  11. Armbrecht HJ, Boltz MA, Kumar VB. Intestinal plasma membrane calcium pump protein and its induction by 1,25-dihydroxyvitamin D3 decrease with age. Am J Physiol Gastrointest Liver Physiol 1999;277:41-7. https://doi.org/10.1152/ajpgi.1999.277.1.G41
  12. Xu H, Bai L, Collins JF, Ghishan FK. Age-dependent regulation of rat intestinal type IIb sodium phosphate cotransporter by 1,25-(OH)2 vitamin D3. Am J Physiol Cell Physiol 2002; 282:487-93. https://doi.org/10.1152/ajpcell.00412.2001
  13. Pardo VG, Boland R, de Boland AR. Vitamin D receptor levels and binding are reduced in aged rat intestinal subcellular fractions. Biogerontology 2008;9:109-18. https://doi.org/10.1007/s10522-007-9118-2
  14. Ministry of Agriculture of the People's Republic of China. Feeding standard of chicken (NY/T 33-2004). Beijing, China: China Agricultural Press; 2004.
  15. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  16. SAS Institute. SAS user's guide. Version 9 ed. Cary, NC, USA: SAS Inst. Inc.; 2002.
  17. Murawska D, Kleczek K, Wawro K, Michalik D. Age-related changes in the percentage content of edible and non-edible components in broiler chickens. Asian-Australas J Anim Sci 2011;24:532-9. https://doi.org/10.5713/ajas.2011.10112
  18. Bond PL, Sullivan TW, Douglas JH, Robeson LG. Influence of age, sex, and method of rearing on tibia length and mineral deposition in broilers. Poult Sci 1991;70:1936-42. https://doi.org/10.3382/ps.0701936
  19. Babatunde OO, Cowieson AJ, Wilson JW, Adeola O. Influence of age and duration of feeding low-phosphorus diet on phytase efficacy in broiler chickens during the starter phase. Poult Sci 2019;98:2588-97. https://doi.org/10.3382/ps/pez014
  20. National Research Council (NRC). Nutrient requirements of poultry. 9th rev ed. Washington, DC, USA: National Acadmies Press; 1994.
  21. Li J, Yuan J, Miao Z, Guo Y. Effects of age on intestinal phosphate transport and biochemical values of broiler chickens. Asian-Australas J Anim Sci 2017;30:221-8. https://doi.org/10.5713/ajas.16.0540
  22. Gloux A, Le Roy N, Brionne A, et al. Candidate genes of the transcellular and paracellular calcium absorption pathways in the small intestine of laying hens. Poult Sci 2019;98:6005-18. https://doi.org/10.3382/ps/pez407
  23. Armbrecht HJ, Boltz MA, Bruns MEH. Effect of age and dietary calcium on intestinal calbindin D-9k expression in the rat. Arch Biochem Biophys 2003;420:194-200. https://doi.org/10.1016/j.abb.2003.09.025
  24. Armbrecht HJ, Boltz MA, Wongsurawat N. Expression of plasma membrane calcium pump mRNA in rat intestine: effect of age and 1,25-dihydroxyvitamin D. Biochim Biophys Acta Biomembr 1994;1195:110-4. https://doi.org/10.1016/0005-2736(94)90016-7
  25. Wang X, Li P, Zhao J, Jiao H, Lin H. The temporal gene expression profiles of calcium and phosphorus transporters in Hy-Line Brown layers. Poult Sci 2022;101:101736. https://doi.org/10.1016/j.psj.2022.101736
  26. Centeno VA, Barboza GEDD, Marchionatti AM, et al. Dietary calcium deficiency increases Ca2+ uptake and Ca2+ extrusion mechanisms in chick enterocytes. Comp Biochem Physiol A Mol Integr Physiol 2004;139:133-41. https://doi.org/10.1016/j.cbpb.2004.08.002
  27. Ichida Y, Ohtomo S, Yamamoto T, et al. Evidence of an intestinal phosphate transporter alternative to type IIb sodiumdependent phosphate transporter in rats with chronic kidney disease. Nephrol Dial Transplant 2021;36:68-75. https://doi.org/10.1093/ndt/gfaa156