Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Forecasting Leaf Mold and Gray Leaf Spot Incidence in Tomato and Fungicide Spray Scheduling

토마토 재배에서 점무늬병 및 잎곰팡이병 발생 예측 및 방제력 연구

  • Lee, Mun Haeng (Fruit Vegetable Research Institute Chungnam-do A.R.E.S)
  • 이문행 (충남농업기술원 과채연구소)
  • Received : 2022.09.01
  • Accepted : 2022.10.18
  • Published : 2022.10.31
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Abstract

The current study, which consisted of two independent studies (laboratory and greenhouse), was carried out to project the hypothesis fungi-spray scheduling for leaf mold and gray leaf spot in tomato, as well as to evaluate the effect of temperature and leaf wet duration on the effectiveness of different fungicides against these diseases. In the first experiment, tomato leaves were infected with 1 × 104 conidia·mL-1 and put in a dew chamber for 0 to 18 hours at 10 to 25℃ (Fulvia fulva) and 10 to 30℃ (Stemphylium lycopersici). In farm study, tomato plants were treated for 240 hours with diluted (1,000 times) 30% trimidazole, 50% polyoxin B, and 40% iminoctadine tris (Belkut) for protection of leaf mold, and 10% etridiazole + 55% thiophanate-methyl (Gajiran), and 15% tribasic copper sulfate (Sebinna) for protection of gray leaf spot. In laboratory test, leaf condensation on the leaves of tomato plants were emerged after 9 hrs. of incubation. In conclusion, the incidence degree of leaf mold and gray leaf spot disease on tomato plants shows that it is very closely related to formation of leaf condensation, therefore the incidence of leaf mold was greater at 20 and 15℃, while 25 and 20℃ enhanced the incidence of gray leaf spot. The incidence of leaf mold and gray leaf spot developed 20 days after inoculation, and the latency period was estimated to be 14-15 days. Trihumin fungicide had the maximum effectiveness up to 168 hours of fungicides at 12 hours of wet duration in leaf mold, whereas Gajiran fungicide had the highest control (93%) against gray leaf spot up to 144 hours. All the chemicals showed an around 30-50% decrease in effectiveness after 240 hours of treatment. The model predictions in present study could be help in timely, effective and ecofriendly management of leaf mold disease in tomato.

이번 연구는 토마토잎곰팡이병 및 점무늬병에 대한 방제력을 개발하고, 온도와 잎의 결로시간에 따른 발생정도를 평가하기 위해 수행되었습니다. 첫 번째 병발생 예측 실험에서는 토마토묘에 Fulvia fulva, Stemphylium lycopersici 분생포자를 각각 1 × 104 conidia·mL-1로 접종시키고 10-25℃(F. fulva) 및 10-30℃(S. lycopersici)에서 0-18시간 동안 이슬생육상에 두었다. 살균제를 이용한 방제 연구에서는 잎곰팡이병 방제를 위하여 트리미다졸, 폴리옥신 B, 이미녹타딘 트리스(Belkut)를 처리하였으며 점무늬병 방제를 위하여서는 에트리디아졸 + 티오파네이트(가지란)과 삼염기 황산구리(세빈나)를 처리하였다. 엽결로시간이 9시간 이상에서 잎곰팡이와 점무니병이 발생하였으며 결로시간이 길어질수록 병발생률이 높았다. 잎곰팡이병의 발생률은 20℃와 15℃에서 더 높았고, 점무늬병은 25℃와 20℃에서 발병률이 증가하였다. 포자 접종 14일 후에 잎곰팡이병 및 점무늬병이 발생하였으며 잠복기는 14-15일로 추정되었다. 잎곰팡이 및 점무늬병 포자가 처리된 식물체에 접종 후 0시간부터 240시까지 살균제 처리를 한 결과 살균제 종류와 상관없이 일찍 약제를 처리한 식물체에서 방제가가 높았으며 150시간이 지난 처리에서는 방제가가 급격히 떨어졌다. 이번연구에서 개발된 병예측모델과 방제력은 토마토의 잎곰팡이병과 점무늬병을 적기에 방제하고 효과적으로 관리하는 데 도움이 될 수 있을 것이다.

Keywords

Acknowledgement

The Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET) and the Korea Smart Farm R&D Foundation (KosFarm) funded this research through the Smart Farm Innovation Technology Development Program, which is funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) and the Ministry of Science and ICT (MSIT), Rural Development Administration (RDA) (421005-04).

References

  1. Ahn J.H., Y.I. Hahm, and E.W. Park 1998a, Development of 'moving average method' for prediction of initial appearance of potato late blight. Korean J Plant Pathol 14:34-40.
  2. Ahn J.H., Y.I. Hahm, and K.Y. Shin 1998b, Modeling for prediction of potato late blight (Phytophthora infestans) progress. Korean J Plant Pathol 14:331-338.
  3. Anonymous 1947, Investigation if air born conidia dispersal of rice blast and Helminthosporium leaf spot pathogens in day and night time. Cent Agric Technol Inst Ann Res Rep, p.314.
  4. Bierhuizen J.F., and W.A. Wagenvoort 1977, Some aspects of seed germination in vegetables, 1. The determination and application of heat sums and minimum temperature for germination. Sci Hortic 2:213-219. doi:10.1016/0304-4238(74)90029-6
  5. Cabral R.N., W.A. Marouelli, D.A.C. Lage, and A.C. Cafe-Filho 2013, Septoria leaf spot in organic tomatoes under diverse irrigation systems and water management strategies. Hortic Bras 31:392-400. doi:10.1590/S0102-05362013000300009
  6. Gleason M.L., A.A. MacNab, R.E. Pitblado, M.D. Ricker, D.A. East, and R.X. Latin 1995, Disease warning systems for processing tomatoes in eastern North America: Are we there yet?. Plant Dis 79:113-121. doi:10.1094/PD-79-0113
  7. Hahm Y.I., B.H. Hahn, and J.D. Franckowiak 1978, Forecasting late blight of potatoes at the alpine area in Korea. Korean J Plant Prot 17:81-87. (in Korean)
  8. Hwang B.S., J.I. Yun, and K.H. Lee 1996, Using hourly weather data to determine dew periods of potato crops. Korean J Plant Pathol 12:445-452. (in Korean)
  9. Jallow M.F.A., D.G. Awadh, M.S. Albaho, V.Y. Devi, and B.M. Thomas 2017, Pesticide risk behaviors and factors influencing pesticide use among farmers in Kuwait. Sci Total Environ 574:490-498. doi:10.1016/j.scitotenv.2016.09.085.
  10. Korean Society of Plant Pathology 2009, List of plant diseases in Korea, 5th ed., p 853. (in Korean)
  11. Lee M.H., S.K. Cho, Y.S. Kim, and D.J. Lee 2017, Study of forecasting and Scheduling for fungicide sprays to control of late blight in tomato. J Korean Soc Int Agric 29:454-458. (in Korean) https://doi.org/10.12719/KSIA.2017.29.4.454
  12. Liu J., and X. Wang 2020, Early recognition of tomato gray leaf spot disease based on MobileNetv2-YOLOv3 model. Plant Methods 16:83. doi:10.1186/s13007-020-00624-2.
  13. MacNab A.A., and R.G. Gardner 1993, Early blight defoliation on tomatoes associated with cultivar and fungicide treatments. Biol Cult Tests 8:54.
  14. Nita M., M.A. Ellis, and L.V. Madden 2003, Effects of temperature, wetness duration, and leaflet age on infection of strawberry foliage by Phomopsis obscurans. Plant Dis 87:579-584. doi:10.1094/PDIS.2003.87.5.579
  15. Park E.W., J.S. Hur, and S.C. Yun 1992, A Forecasting system for scheduling fungicide sprays to control grape ripe rot caused by Colletotrichum gloeosporioides. Korean J Plant Pathol 8:177-184. (in Korean)
  16. Pitblado R.E. 1988, Development of a weather-timed fungicide spray program for field tomatoes. Can J Plant Pathol 10:371.
  17. Pitblado R.E. 1992, The development and implementation of TOM-CAST, a weather timed fungicide spray program for field tomatoes. Ministry of Agriculture and Food, Ontario, Canada.
  18. Sikora E.J., E.M. Bauske, G.W. Zehnder, and M.H. Hollingsworth 1994, Evaluation of low-input fungicide spray programs for control of early blight on tomatoes. Highlights of Agricultural Research.
  19. Wang H., J.A. Sanchez-Molina, M. Li, and M. Berenguel 2020, Development of an empirical tomato crop disease model: a case study on gray leaf spot. Eur J Plant Pathol 156: 477-490. doi:10.1007/s10658-019-01897-7
  20. Xu L., A. Lu, J. Wang, Z. Ma, L. Pan, X. Feng, and Y. Luan 2015, Accumulation status, sources and phytoavailability of metals in greenhouse vegetable production systems in Beijing, China. Ecotoxicol Environ Saf 122:214-220. doi:10.1016/j.ecoenv.2015.07.025