한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제39권4호
  • /
  • Pages.580-587
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  • 2022
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  • 1225-9098(pISSN)
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  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
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쥐참외뿌리 추출물의 항산화, 미백, 항염증 활성 연구

A Study on the Antioxidant, Whitening and Anti-Inflammatory Activities of Trichosanthis Cucumeroidis Radix Extract

  • 유선희 (연성대학교 뷰티스타일리스트과 스킨케어전공) ;
  • 이은주 (연성대학교 뷰티스타일리스트과 스킨케어전공)
  • You, Seon-Hee (Department of Beauty Stylist Major in Skin Care, Yeonsung University) ;
  • Lee, Eun-Ju (Department of Beauty Stylist Major in Skin Care, Yeonsung University)
  • 투고 : 2022.07.31
  • 심사 : 2022.08.28
  • 발행 : 2022.08.30
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초록

본 연구에서는 쥐참외뿌리 추출물의 항산화 활성 및 미백, 항염증 활성에 대한 생리활성 효과와 기능성 소재로써의 활용 가능성을 확인해 보고자 하였다. 쥐참외뿌리 추출물의 DPPH 라디칼 소거 활성을 통한 항산화 활성, 멜라닌 세포인 B16F10 melanoma 세포에 대한 멜라닌 생성 억제능을 통한 미백 활성 효과, 대식 세포인 RAW 264.7 세포에 대한 NO 생성 억제능을 통한 항염증 활성 효과를 확인해 보고자 하였다. 연구결과, 쥐참외뿌리 추출물은 농도 의존적으로 DPPH 라디칼 소거활성이 확인되었으며, 양성 대조군인 Ascorbic acid와 비슷한 DPPH 라디컬 소거활성이 확인되었다. 100 nM 𝛼-MSH로 유도된 멜라닌 생성 억제 활성과 LPS 1 ㎍/mL로 유도된 NO 생성 억제능을 유의하게 억제 하는 것을 확인되었다. 이에 따라 쥐참외뿌리 추출물은 항산화 및 미백, 항염증 효과를 가진 기능성 소재로써의 활용 가능성이 있음이 사료되어 진다.

In this study, the physiological activity effect of trichosanthis cucumeroidis radix extract on antioxidant activity, whitening, and anti-inflammatory activity was checked, and the possibility of its use as a functional material was checked. The purpose of this study was to confirm the antioxidant activity through DPPH radical scavenging activity of trichosanthis cucumeroidis radix extract, whitening activity effect through melanin production inhibition ability for melanin cell B16F10 melanoma cell, and anti-inflammatory activity effect through NO production inhibition ability for macrophage RAW 264.7 cell. As a result of the study, the concentration-dependent DPPH radical scavenging activity of the trichosanthis cucumeroidis radix extract was confirmed, and DPPH radical scavenging activity similar to that of the positive control Ascorbic acid was confirmed. It was confirmed that the melanin production inhibitory activity induced by 100 nM 𝛼-MSH and the NO production inhibitory ability induced by LPS 1 ㎍/mL were significantly suppressed. Accordingly, it is considered that the trichosanthis cucumeroidis radix extract may be used as a functional material having antioxidant, whitening, and anti-inflammatory effects.

키워드

참고문헌

  1. K. Maeda, M. Fukud, "In vitro effectiveness of several whitening cosmetic components in human melanocytes", The Journal of Society of Cosmetic Scientists of Korea, Vol.42, pp. 361-368, (1991).
  2. T. H Lee, H. J. Kim, Y. B. Kim, "Depigmentation Activity of Barley, Unpolished Rice, Job's-tear", The Journal of Oriental Medical Ophthalmology & Otolaryngololgy & Dermatology, Vol.16, No.2 pp. 57-78, (2003).
  3. H. Hwangbo, J. S. Jeung, M. Y. Kim, S. Y. Ji, S. H. Yoon, T. H. Kim, S. O. Kim, Y. H. Choi, "A Study on Antioxidant and Antiinflammatory Effects Based on Analysis of Functional Components of Cornus officinalis Siebold & Zucc", Journal of Life Science, Vol.31, No.3 pp. 287-297, (2021). https://doi.org/10.5352/JLS.2021.31.3.287
  4. J. Lee, N. Koo, D. B. Min, "Reactive oxygen species, aging, and antioxidative nutraceuticals", Comprehensive reviews in food science and food safey, Vol.3, No.1 pp. 21-33, (2004). https://doi.org/10.1111/j.1541-4337.2004.tb00058.x
  5. H. Kang, S. G. Lee, "Antioxidant Capacity of Ethanol Extracts and Fractions from Rubus coreanus Miq", Journal of Plant Biotechnology, Vol.48, pp. 264-270, (2021). https://doi.org/10.5010/JPB.2021.48.4.264
  6. M. J. Jang, S. J. Seo, Y. S. Lee, "Antioxidant, Anti-inflammatory and Anti-aging Activities of Elaeagnus multiflora Fruit Extracts as a Cosmetic Material", Journal of Investigative Cosmetology, Vol.17, No.2 pp. 159-167, (2021). https://doi.org/10.15810/JIC.2021.17.2.006
  7. I. Gulcin I, "Antioxidants and antioxidant methods: An updated overview", Archives of toxicology, Vol.94, No.3 pp. 651-751, (2020). https://doi.org/10.1007/s00204-020-02689-3
  8. J. B. Park, K. M. Lee, J. S. Park, J. Kim, J. D. Lee, "Selection of high functional red paprika (Capsicum annuum L.) germplasms by antioxidant activity, polyphenol content, and red pigment amount", Korean J. Breed. Sci, Vol.53, pp.96-104, (2021). https://doi.org/10.9787/KJBS.2021.53.2.96
  9. H. J. Kwon, S. H. Beom, J. A. Hyun, E. B. Kang, H. E. Park, D. G. Han, E. Y. Choi, B. J. An, "Analysis of antioxidant activity, total phenol content, and flavonoid content of Abelmoschus manihot flower extracts", Korean J. Food Preserv, Vol.29, pp. 157-165, (2022). https://doi.org/10.11002/kjfp.2022.29.1.157
  10. N. Guo, J. A. Lee, "Evaluation of antioxidant, anti-inflammatory, and antiwrinkle effects of the mushroom Leucopaxillus giganteus", Korean Society of Cosmetics and Cosmetology. Vol.12, No.1, pp. 113-120, (2022).
  11. J. W. Hwang, H. Kang, S G. Lee, "Antioxidant and anti-inflammatory effects of seed ethanol extracts of Rubus coreanus miquel", J Plant Biotechnol, Vol.49, pp. 155-161, (2022). https://doi.org/10.5010/JPB.2022.49.2.155
  12. H. K. Oh, "Antioxidant and Antiinflammatory Activities of Atractylodes japonica According to Extract Methods", Journal of the Korean Applied Science and Technology, Vol.38, No.6 pp. 1543-1552, (2021). https://doi.org/10.12925/JKOCS.2021.38.6.1543
  13. J. C. Yang, "A Study on the Antioxidant and Anti-inflammatory Effects of Prunella vulgaris Extract", Culture and Convergence, Vol.43, No.11 pp. 1037-1048, (2021).
  14. K. C. Kim, J. S. kim, "Antioxidant Activities and Phenolic Compounds of Extract from Hallasan Wormwood", korean journal medicinal crop science, Vol.29, No.5 pp. 345-352, (2021). https://doi.org/10.7783/KJMCS.2021.29.5.345
  15. Department of Dermatology. Seoul National University College of Medicine. Dermatology for medical students. 4th edition. Seoul;Korea Medical Book Publishing Co. pp. 237-243, (2017).
  16. S. N. Youn, Y. J. Kim, Y. J. Lee, M. R. Kim, W. K. Yoo, "Effect of ethanol extract from mixture including Angelicae Dahuricae Radix on Dermal Anti-aging and Whitening", The Korea Jounrnal of Herbology, Vol.34, No.6 pp. 109-115, (2019).
  17. J. C. Lee, S. Y. Park, J. H. Choi, J. H. Kim, "Effects of Paeoniae radix alba(PRA) on Skin whitening and Elasticity using Melanoma cells", The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology, Vol.25, No.1 pp.1-11, (2012).
  18. M. S. Ko, H. J. Lee, M. J. Kang, "Antioxidant Activities and Whitening Effects of Extracts from Hippophae rhamnoides L," J East Asian Soc Dietary Life, Vol.22, No.6 pp. 812-817, (2012).
  19. S. J. Yang, T. B Choe, "Antioxidant Activity and Whitening Effect of Forsythiae Fructus Extracts", Korean J. Medicinal Crop Sci, Vol.19, No.6 pp. 472-477, (2011). https://doi.org/10.7783/KJMCS.2011.19.6.472
  20. M. J. Seong, J. E. Kim, N. H. Lee, "Whitening and Anti-oxidative Constituents from the Extracts of Hydrangea petiolaris Leaves", journal of the society of cosmetic scientists of korea, Vol.48, No.2 pp. 123-134, (2022). https://doi.org/10.15230/SCSK.2022.48.2.123
  21. M. S. Blois, "Antioxidant determinations by the use of a stable free radical", Nature. Vol.181, pp. 1199-1200, (1958). https://doi.org/10.1038/1811199a0
  22. H. Kang, S. G. Lee, "Antioxidant capacity of ethanol extracts and fractions from Rubus coreanus Miq", J. Plant Biotechnol, Vol.48, pp. 264-270, (2021). https://doi.org/10.5010/JPB.2021.48.4.264
  23. S. H. You, J. S. Moon, "Antioxidant and Cytotoxicity in Skin Cell of the Trichosanthis Cucumeroidis Radix Extract", Journal of the Korean Applied Science and Technology, Vol.39, No.3 pp. 417-422, (2022). https://doi.org/10.12925/JKOCS.2022.39.3.417
  24. M. J. Choi, "Skin Whitening Efficacy of Mixed Extracts of Crassostrea gigas, Trichosanthes cucumeroides, Angelica dahurica and Asarum sieboldii", Asian J Beauty Cosmetol, Vol.20, No.2 pp. 157-167, (2022). https://doi.org/10.20402/ajbc.2021.0283