The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory effect of Uncariae Ramulus et Uncus on alcohol-induced gastritis

알코올성 위염에 대한 조구등(釣鉤藤)의 항염증 효과

  • Lee, Jin A (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Tae Jong (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jin Young (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이태종 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김진영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 박해진 (DHU 바이오융복합시험센터) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2022.08.02
  • Accepted : 2022.09.25
  • Published : 2022.09.30
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Abstract

Objective : Gastritis refers to an inflammatory disease of the gastric mucosa. Alcohol is one of the main aggression factors, causing bleeding and inflammation in the gastric mucosa and it is known to not only increase lipid peroxide levels, but also deplete key antioxidant factors. The purpose of this study was to determine the effect of Uncariae Ramulus et Uncus water extract (URW) in alcohol-induced gastritis. Methods : The total polyphenol and flavonoid contents of URW were confirmed through an in vitro experiment. Also, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) activity were confirmed. For in vivo experiments, mice were divided into 4 groups (n=8). Also, 1 hr after oral administration of each drug, 50% ethanol was orally administered to induce gastritis. Results : As a result of in vitro experiments, URW showed excellent antioxidant activity. In alcohol-induced gastritis, URW alleviated the damage to the gastric mucosa caused by alcohol. Also, URW decreased reactive oxygen species (ROS) and malondialdehyde (MDA) levels in serum and gastric tissues, and significantly decreased the expression of NADPH oxidases in gastric tissues. In addition, it significantly modulated the nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and nuclear factor-𝜅B p65 (NF-𝜅B) pathways as well as significantly increased the expression of anti-inflammatory proteins. Conclusions : These results suggest that URW not only reduces oxidative stress through excellent antioxidant activity but also relieves gastric mucosal inflammation as a regulator of Nrf2 and NF-𝜅B pathways.

Keywords

Acknowledgement

이 연구는 2022년도 정부 (과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행되었습니다 (No. 2018R1A5A2025272).

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