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http://dx.doi.org/10.6116/kjh.2022.37.5.63.

Anti-inflammatory effect of Uncariae Ramulus et Uncus on alcohol-induced gastritis  

Lee, Jin A (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Lee, Tae Jong (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Kim, Jin Young (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Park, Hae-Jin (DHU Bio Convergence Testing Center)
Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.37, no.5, 2022 , pp. 63-74 More about this Journal
Abstract
Objective : Gastritis refers to an inflammatory disease of the gastric mucosa. Alcohol is one of the main aggression factors, causing bleeding and inflammation in the gastric mucosa and it is known to not only increase lipid peroxide levels, but also deplete key antioxidant factors. The purpose of this study was to determine the effect of Uncariae Ramulus et Uncus water extract (URW) in alcohol-induced gastritis. Methods : The total polyphenol and flavonoid contents of URW were confirmed through an in vitro experiment. Also, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) activity were confirmed. For in vivo experiments, mice were divided into 4 groups (n=8). Also, 1 hr after oral administration of each drug, 50% ethanol was orally administered to induce gastritis. Results : As a result of in vitro experiments, URW showed excellent antioxidant activity. In alcohol-induced gastritis, URW alleviated the damage to the gastric mucosa caused by alcohol. Also, URW decreased reactive oxygen species (ROS) and malondialdehyde (MDA) levels in serum and gastric tissues, and significantly decreased the expression of NADPH oxidases in gastric tissues. In addition, it significantly modulated the nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and nuclear factor-𝜅B p65 (NF-𝜅B) pathways as well as significantly increased the expression of anti-inflammatory proteins. Conclusions : These results suggest that URW not only reduces oxidative stress through excellent antioxidant activity but also relieves gastric mucosal inflammation as a regulator of Nrf2 and NF-𝜅B pathways.
Keywords
Uncariae Ramulus et Uncus; gastritis; antioxidant; oxidative stress; inflammation;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Lee JJ, Choi HS, Lee JH, Jung CJ, Lee MY. The Effects of Ethylacetate Fraction of Sanguisorba officinalis L. on Experimentally-induced Acute Gastritis and Peptic Ulcers in Rats. J Korean Soc Food Sci Nutr. 2005;34(10):1545-52.   DOI
2 Choi GN, Jeong CH, Kim JH, Kawk JH, Shin YH, Lee SC, Cho SH, Choi SG, Heo HJ. Effect of Storage Temperature and Water Activity on Antioxidant Activities of Powdered Green Tea Extracts. Korean J Food Preserv. 2009;16(3):333-41.
3 Park SK, Jin DE, Park CH, Seung TW, Choi SG, Heo HJ. PC12 Cell Protective Effects of Broccoli (Brassica oleracea var. italica) Leaf Fraction against H2O2-induced Oxidative Stress. Korean J Food Sci Technol. 2014;46(4):483-8.   DOI
4 Lee JA, Shin MR, Lee JH, Park HJ, Roh SS. The Effect of Chaenomelis Fructus on Thioacetamide-Induced Acute Hepatic Injury. J Korean Soc Food Sci Nutr. 2021;50(4):322-9.   DOI
5 Lee JA, Shin MR, Choi JW, Roh SS. Effect of Toosendan Fructus on Chronic Acid Reflux Esophagitis Rats. Kor J Herbol. 2021;36(3):1-8.
6 Kim KS, Hong KE, Yim YK. A study on the anti-oxidative effectof electroacupuncture at Wijung (BL40) in rats. Korean J Acupunct. 2007;24(1):145-60.
7 He HJ, Wang GY, Gao Y, Ling WH, Yu ZW, Jin TR. Curcumin attenuates Nrf2 signaling defect, oxidative stress in muscle and glucose intolerance in high fat diet-fed mice. Worl J Diabetes. 2012;3(5):94-104.   DOI
8 Rushworth SA, MacEwan DJ, O'Connell MA. Lipopolysaccharide-Induced Expression of NAD(P)H: Quinone Oxidoreductase 1 and Heme Oxygenase-1 Protects against Excessive Inflammatory Responses in Human Monocytes. J Immunol. 2008;181(10):6730-7.   DOI
9 Moriyama I, Ishihara S, Rumi MA, Aziz MD, Mishima Y, Oshima N, Kadota C, Kadowaki Y, Amano Y, Kinoshita Y. Decoy oligodeoxynucleotide targeting activator protein-1 (AP-1) attenuates intestinal inflammation in murine experimental colitis. Lab Invest. 2008;99(6):652-63.
10 Hernandez-Munoz R, Montiel-Ruiz C, Vazquez-Martinez O. lab invest. 2000;80(8):1161-9.   DOI
11 Suzuki H, Nishizawa T, Tsugawa H, Mogami S, Hibi T. Roles of oxidative stress in stomach disorders. J Clin Biochem Nutr. 2012;50(1):35-9.
12 Kang MH, Jeong CS. Effects of Amomi Semen Butanol Fraction an Gastritis and Helicobacter pylori. Kor J Pharmacogn. 2004;35(4):350-6.
13 Zhou Q, Ma J, Chen L. Tissue Distribution of Hirsutine and Hirsuteine in Mice by Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry. J Anal Methods Chem. 2020;2020:7204315.
14 Kim JH, Bae CH, Park SY, Lee SJ, Kim Y. Uncaria rhynchophylla inhibits the production of nitric oxide and interleukin-1β through blocking nuclear factor κB, Akt, and mitogen-activated protein kinase activation in macrophages. J Med Food. 2010;13(5):1133-40.   DOI
15 Zhou Z, Su Y, Fa XE. Isorhynchophylline exerts anti-inflammatory and anti-oxidative activities in LPS-stimulated murine alveolar macrophages. Life Sci. 2019;223:137-45.   DOI
16 Somogyi A, Rosta K, Pusztai P, Tulassay Z, Nagy G. Antioxidant measurements. Physiol Meas. 2007;28(4):R41-55.   DOI
17 Kushida H, Matsumoto T, Ikarashi Y. Properties, Pharmacology, and Pharmacokinetics of Active Indole and Oxindole Alkaloids in Uncaria Hook. Front Pharmacol. 2021;12:688670.   DOI
18 Vestergaard P, Rejnmark L, Mosekilde L. Proton pump inhibitors, histamine H2 receptor antagonists, and other antacid medications and the risk of fracture. Calcif Tissue Int. 2006;79:76-83.   DOI
19 Ju YS. Ungok Herbology. Seoul : Woosuk. 2013:1670.
20 Tian Z, Zhang S, Wang H, Chen Z, Sun M, Sun L, Gong L, Li Y, Jiang H. Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats. Front Pharmacol. 2020;11:910.   DOI
21 Nunes CDR, Barreto Arantes M, Menezes de Faria Pereira S, Leandro da Cruz L, de Souza Passos M, Pereira de Moraes L, Vieira IJC, Barros de Oliveira D. Plants as Sources of Anti-Inflammatory Agents. Molecules. 2020;25(16):3726.   DOI
22 Rama P, Vignesh A, Lakshmanan G. In vitro antioxidant activity of achyranthes aspera linn. Int Med Pharm. 2013;3(2):37-78.
23 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9-10):1231-7.   DOI
24 Jiao H, Wang SY. Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. J Agric Food Chem. 2000;48(11):5672-6.   DOI
25 Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199-200.   DOI
26 Rahman MM, Islam MB, Biswas M, Khurshid Alam AH. In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res Notes. 2015;8:621.   DOI
27 Zhang Y, Cui Y, Dai S, Deng W, Wang H, Qin W, Yang H, Liu H, Yue J, Wu D, Wang J, Guo H. Isorhynchophylline enhances Nrf2 and inhibits MAPK pathway in cardiac hypertrophy. Naunyn Schmiedebergs Arch Pharmacol. 2020;393(2):203-12.   DOI
28 Lee JA, Kim SH, Kim MJ, Ahn JH, Park HJ, Lee WR, Roh SS. Protective Effects of Chrysanthemi Indici Flos Extract and Flaxseed Oil Mixture on HCl/ethanol-induced Acute Gastric Lesion Mice. Kor J Herbol. 2018;33(6):19-28.
29 Bagchi D, Carryl OR, Tran MX, Krohn RL, Bagchi DJ, Garg A, Bagchi M, Mitra S, Stohs SJ. Stress, diet and alcohol-induced oxidative gastrointestinal mucosal injury in rats and protection by bismuth subsalicylate. J Appl Toxicol. 1998;18(1):3-13.   DOI
30 Yeo D, Hwang SJ, Kim WJ, Youn HJ, Lee HJ. The aqueous extract from Artemisia capillaris inhibits acute gastric mucosal injury by inhibition of ROS and NF-kB. Biomed Pharmacother. 2018;99:681-7.   DOI
31 Ali SF, LeBel CP, Bondy SC. Reactive oxygen species formation as a biomarker of methylmercury and trimethyltin neurotoxicity. Neurotoxicology. 1992 ; 13 : 637-648.
32 Kim JH, Li L, Kim MS, Cho EJ, Kim HY, Choi JS. Hesperidin and Hesperetin Protect against Oxidative Stresson Hepatic Toxicity in Rats. J Korean Med Obes Res. 2022;22(1):1-10.   DOI
33 Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978;86(1):271-8.   DOI
34 Oh GH, Nam JB, Yang SM, Joung WH, Jeong JS, Shin JM, Kang SG. A Study on the Antioxidant Effectiveness of the Extracts from Superheated Steaming Torrefied Wood. J of Korea TAPPI. 2018;50(2):5-12.
35 Lee JA, Shin MR, Lee SN, Park SA, Park HJ. Effect of a Mixture of Rhei Rhizoma and Scutellariae Radix Extract on Acute Reflux Esophagitis Rats. Kor J Herbol. 2020;35(6):43-53.
36 Gao W, Guo L, Yang Y, Wang Y, Xia S, Gong H, Zhang BK, Yan M. Dissecting the Crosstalk Between Nrf2 and NF-κB Response Pathways in Drug-Induced Toxicity. Front Cell Dev Biol. 2022;9:809952.   DOI