The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The comparative study on the anti-obesity effect of Arctium lappa L. roots with and without microwave processing

마이크로파 처리 유무에 따른 우엉뿌리의 항비만 효과 비교 연구

  • Seo, Seong-Wook (Department of Rehabilitation Sciences, Graduate School, Daegu University) ;
  • Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Kyoung (Department of Physical Therapy, College of Rehabilitation Sciences, Daegu University) ;
  • Chung, Dong-Min (ShinWoo co.) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 서성욱 (대구대학교 대학원 재활과학과) ;
  • 김민주 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김경 (대구대학교 재활과학대학 물리치료학과) ;
  • 정동민 ((주)신우코퍼레이션) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2022.06.13
  • Accepted : 2022.07.25
  • Published : 2022.07.30
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Abstract

Objective : The present study comparatively analyzed the anti-obesity effect of Arctium lappa L. roots with and without microwave processing. Methods : Four HFD groups except for the Normal group (n=8) were allocated: Control, microwave-processed dried Arctium lappa L. roots (MAL) extract 400 mg/kg/d (MAL400), MAL extract 800 mg/kg/d (MAL800), and the dried Arctium lappa L. roots (DAL) extract 800 mg/kg/d (DAL800). The efficacy of MAL and DAL was confirmed in terms of the serum biochemical index, protein expressions related to the synthesis of triglyceride (TG) and total cholesterol (TC) and 𝛽-oxidation, and histopathological staining. Results : Both MAL and DAL treatments significantly reduced final body weight and body weight gain. MAL800 treatment significantly reduced serum TG, TC, low-density lipoprotein cholesterol, and leptin levels, but the serum high-density lipoprotein cholesterol and adiponectin concentrations were dramatically increased. In particular, leptin in the MAL800 group was reduced by 14.1% compared with the DAL800 group. Moreover, the MAL800 treatment showed an effect superior to the DAL800 treatment in the reduction of serum TC, the sirtuin 1 (Sirt1) activation, and the inhibition of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGCR) gene expression. In particular, unlike the DAL800 treatment, MAL treatment significantly led to the activation of peroxisome proliferator-activated receptor alpha (PPAR𝛼). Subsequently, PPAR𝛼 meaningfully regulated downstream proteins associated with 𝛽-oxidation. Conclusion : These findings suggest that Arctium lappa L. roots with microwave processing effectively ameliorate obesity through the regulation of leptin and TC and the promotion of 𝛽-oxidation compared with Arctium lappa L. roots without microwave processing.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2018R1A5A2025272).

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