Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Distribution of resistance genes against lincomycin of pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus)

양식 넙치에서 분리한 어병세균의 lincomycin에 대한 내성 유전자의 분포

  • Kim, Ye Ji (Department of Marine Life Sciences, Jeju National University) ;
  • Jun, Lyu Jin (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Young Juhn (Department of Marine Life Sciences, Jeju National University) ;
  • Ko, Ye Jin (Department of Marine Life Sciences, Jeju National University) ;
  • Han, So Ri (Fishcare laboratory) ;
  • Kim, Sung Hyun (Fishcare laboratory) ;
  • Jeong, Joon Bum (Department of Marine Life Sciences, Jeju National University)
  • 김예지 (제주대학교 해양생명과학과) ;
  • 전려진 (제주대학교 해양생명과학과) ;
  • 이영준 (제주대학교 해양생명과학과) ;
  • 고예진 (제주대학교 해양생명과학과) ;
  • 한소리 (피쉬케어연구소) ;
  • 김성현 (피쉬케어연구소) ;
  • 정준범 (제주대학교 해양생명과학과)
  • Received : 2022.04.29
  • Accepted : 2022.05.27
  • Published : 2022.06.30
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Abstract

Lincomycin as one of the lincosamides antibiotics have been mainly used in human and livestock fields, but have not been used in aquaculture. In this study, the distribution of minimum inhibitory concentration (MIC) values against lincomycin and the detection of the macrolide-lincosamide-streptogramin (MLS) resistance gene were confirmed in bacterial pathogens isolated from cultured olive flounder (Paralichthys olivaceus). Of the 107 strains isolated from Jeju, 36 strains of Gram-positive bacteria and 71 strains of Gram-negative bacteria were identified. Most of Streptococcus spp. was found to have a MIC value of less than or equal to 0.5 ㎍/mL, and Edwardsiella piscicida was found to have a MIC value higher than 1,024 ㎍/mL. V. harveyi and V. alginolyticus mostly showed MIC values of 256 ㎍/mL, but V. scophthalmi displayed values of 8~64 ㎍/mL. In the detection of MLS resistance gene, erm(B) was detected in 9 strains of Streptococcus spp., and erm(A) was confirmed in one strain.

Keywords

Acknowledgement

이 논문은 2021학년도 제주대학교 교원성과지원사업에 의하여 연구되었음.

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