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Molecular identification and phylogenetic analysis of Neobenedenia spp. isolated from small yellow croaker (Larimichthys polyactis)

참조기(Larimichthys polyactis)에서 분리된 Neobenedenia spp.에 대한 분자 생물 동정 및 계통수 분석

  • Seo, Han-Gill (South Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Hyo-Won (South Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Jung-Hyun (Jeju Fisheries Research Institute, National Institute of Fisheries Sci)
  • 서한길 (국립수산과학원 남해수산연구소) ;
  • 김효원 (국립수산과학원 남해수산연구소) ;
  • 김정현 (국립수산과학원 제주수산연구소)
  • Received : 2022.03.21
  • Accepted : 2022.05.25
  • Published : 2022.06.30

Abstract

In this study, we determined the cause of a disease outbreak in small yellow croaker(Larimichthys polyactis) in Jeju island. The major external signs in the dead fish were hemorrhage of the skin. Vibrio harveyi were isolated from a few fishes and viruses were not detected from the diseased fish. However, flukes were confirmed on the skin and we conducted molecular identification and phylogenetic analysis of the isolated parasites. The obtained 28S rRNA sequence of our specimen(Accession No. OM333244) showed the highest homology with Neobenedenia girellae, while the COI sequence of our specimen showed the highest homology with N. melleni. Further sequence analysis with other genes and morphological observation are necessary for accurate identification.

Keywords

Acknowledgement

본 연구는 국립수산과학원(고수온 대응 양식품종 교잡바리류 양성기술 연구, R2022004)의 지원에 의해 수행되었습니다.

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