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http://dx.doi.org/10.7847/jfp.2022.35.1.135

Molecular identification and phylogenetic analysis of Neobenedenia spp. isolated from small yellow croaker (Larimichthys polyactis)  

Seo, Han-Gill (South Sea Fisheries Research Institute, National Institute of Fisheries Science)
Kim, Hyo-Won (South Sea Fisheries Research Institute, National Institute of Fisheries Science)
Kim, Jung-Hyun (Jeju Fisheries Research Institute, National Institute of Fisheries Sci)
Publication Information
Journal of fish pathology / v.35, no.1, 2022 , pp. 135-140 More about this Journal
Abstract
In this study, we determined the cause of a disease outbreak in small yellow croaker(Larimichthys polyactis) in Jeju island. The major external signs in the dead fish were hemorrhage of the skin. Vibrio harveyi were isolated from a few fishes and viruses were not detected from the diseased fish. However, flukes were confirmed on the skin and we conducted molecular identification and phylogenetic analysis of the isolated parasites. The obtained 28S rRNA sequence of our specimen(Accession No. OM333244) showed the highest homology with Neobenedenia girellae, while the COI sequence of our specimen showed the highest homology with N. melleni. Further sequence analysis with other genes and morphological observation are necessary for accurate identification.
Keywords
small yellow croaker; Neobenedenia spp.; 28S rRNA; COI;
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