Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Lipidomic profiling of Skipjack tuna (Katsuwonus pelamis) by ultrahigh-performance liquid chromatography coupled to high resolution mass spectrometry

  • Hu, Lingping (College of Food Science and Technology, Hainan Tropical Ocean University) ;
  • Hu, Zhiheng (College of Food Science and Technology, Hainan Tropical Ocean University) ;
  • Chin, Yaoxian (College of Food Science and Technology, Hainan Tropical Ocean University) ;
  • Yu, Haixia (Ocean Research Center of Zhoushan, Zhejiang University) ;
  • Xu, Jianhong (Zhejiang Retronx Foodstuff Industry Co., Ltd.) ;
  • Zhou, Jianwei (College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University) ;
  • Liu, Donghong (College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University) ;
  • Kang, Mengli (Processing Technology Research, Ningbo Academy of Agricultural Sciences) ;
  • Hu, Yaqin (College of Food Science and Technology, Hainan Tropical Ocean University)
  • Received : 2021.10.15
  • Accepted : 2022.01.13
  • Published : 2022.03.31
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Abstract

A method of ultrahigh performance liquid chromatography coupled to high resolution mass spectrometry (UPLC-HRMS) was established for characterization of the lipid profile of Skipjack tuna. Over 300 lipid molecular species were identified through cross-acquisition in both positive and negative ion mode. Phospholipids (PLs) were dominant in Skipjack tuna. Lysophosphatidylethanolamine (LPE), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC) and phosphatidylcholine (PC) were the main lipid molecular species in PLs, accounting for 89.24% of the total PLs. The ratio of sphingolipids (SLs) and glycerolipids (GLs) were considerable, accounting for 12.30% and 13.60% of the total lipids respectively. Ceramide (Cer) was the main lipid molecular species of SLs, accounting for 64.96% of total SLs, followed by sphingomyelin (SM), accounting for 25.45% of total SLs. Ether diglycerides (ether DG) were the main lipid molecular species of GLs (97.83%). The main fatty acids (FAs) are unsaturated fatty acids (UFAs) in Skipjack tuna. Besides, a new FAs class branched fatty acid esters of hydroxy fatty acids (FAHFA) was detected, together with the FA. The active lipids identified in this study can be used to evaluate the nutritional value of Skipjack tuna.

Keywords

Acknowledgement

The authors are grateful to Jian Ji (Jiangnan University) for his help in the data processing. The authors are also grateful to Ying Shao, Fan Yang and Chen Nie (Technology Center of Qingdao Customs District) for their help in instrument operation.

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