Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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The List of Korean Organisms Registered in the NCBI Nucleotide Database for Environmental DNA Research

환경유전자 연구를 위한 NCBI Nucleotide 데이터베이스에 등록된 국내 생물 목록 현황

  • Ihn-Sil Kwak (Department of Ocean Integrated Science, Chonnam National University) ;
  • Chang Woo Ji (Fisheries Science Institute, Chonnam National University) ;
  • Won-Seok Kim (Department of Ocean Integrated Science, Chonnam National University) ;
  • Dongsoo Kong (Kyonggi University)
  • 곽인실 (전남대학교 해양융합과학과) ;
  • 지창우 (전남대학교 수산과학연구소) ;
  • 김원석 (전남대학교 해양융합과학과) ;
  • 공동수 (경기대학교 생명과학과)
  • Received : 2022.11.07
  • Accepted : 2022.12.17
  • Published : 2022.12.31
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Abstract

Recently, with the development of genetic technology, interest in environmental DNA (eDNA) to study biodiversity according to molecular biological approaches is increasing. Environmental DNA has many advantages over traditional research methods for biological communities distributed in the environment but highly depends on the established base sequence database. This study conducted a comprehensive analysis of the habitat status and classification at the genus level, which is mainly used in eDNA (12S rRNA, 16S rRNA, 18S rRNA, COI, and CYTB), focusing on Korean registration taxon groups (phytoplankton, zooplankton, macroinvertebrates, and fish). As a result, phytoplankton and zooplankton showed the highest taxa proportion in 18S rRNA, and macroinvertebrates observed the highest ratio in the nucleotide sequence database in COI. In fish, all genes except 18S rRNA showed a high taxon ratio. Based on the Korean registration taxon group, the gene construction of the top 20 genera according to bio density observed that most of the phytoplankton were registered in 18S rRNA, and the most significant number of COI nucleotide sequences were established in macroinvertebrates. In addition, it was confirmed that there is a nucleotide sequence for the top 20 genera in 12S rRNA, 16S rRNA, and CYTB in fish. These results provided comprehensive information on the genes suitable for eDNA research for each taxon group.

국내 서식하는 수서 생물(식물플랑크톤, 동물플랑크톤, 저서대형무척추동물, 어류)에 대한 eDNA 연구에 주요 이용되는 유전자인 12S rRNA와 16S rRNA, 18S rRNA, COI, CYTB를 대상으로 속(Genus) 수준의 등록 현황을 조사하였다. 그 결과 식물플랑크톤과 동물플랑크톤은 18S rRNA에서 가장 높은 등록 속 비율을 보였으며, 저서무척추동물은 COI에서 가장 높은 등록 속 비율을 확인하였다. 어류에서는 18S rRNA를 제외한 모든 유전자에서 90%에 가까운 높은 비율을 보였다. 분류군에 따른 우점 생물의 상위 20속에 대한 유전자 등록 현황은 식물플랑크톤은 18S rRNA에서 19속이, 저서무척추동물은 COI에서 18개 속이 등록되어 있었다. 어류에서는 12S rRNA, 16S rRNA, CYTB에서 상위 20의 모든 유전자 염기서열이 존재함을 확인하였다. 본 자료 분석을 통하여 각 분류군별 eDNA 연구에 적합한 유전자 데이터베이스의 양적인 정보를 파악하였다.

Keywords

Acknowledgement

본 결과물은 환경부의 재원으로 한국환경산업기술원 수생태계 건강성 확보 기술개발사업의 지원(과제번호: 2021003050001, 2022003050006)과 한국연구재단의 지원(NRF-2018R1A6A1A03024314)을 받아 연구되었습니다.

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