Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Re-evaluation on conversion system of BactoScan and BactoCount for raw milk in South Korea

국내 원유 세균수 검사장비 박토스캔 및 박토카운트의 보정식 재평가

  • Kim, ESeul (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Kim, Jin-Hwan (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Byun, Yeong-Seob (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Park, Dasom (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Young (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Yoon, Soon-Seek (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Moon, Jin-San (Bacterial Disease Division, Animal and Plant Quarantine Agency)
  • 김이슬 (농림축산검역본부 세균질병과) ;
  • 김진환 (농림축산검역본부 세균질병과) ;
  • 변영섭 (농림축산검역본부 세균질병과) ;
  • 박다솜 (농림축산검역본부 세균질병과) ;
  • 김하영 (농림축산검역본부 세균질병과) ;
  • 윤순식 (농림축산검역본부 세균질병과) ;
  • 문진산 (농림축산검역본부 세균질병과)
  • Received : 2022.01.12
  • Accepted : 2022.03.17
  • Published : 2022.03.30
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Abstract

The total bacteria count is significantly important factor for hygienic quality in raw milk. BactoScan FMTM and BactoCount IBCTM are the automated instruments for the determination of the total bacterial count in raw milk. They have been used after calibration by standard plate count (SPC) method in South Korea since 2000. It is necessary to re-evaluate for total bacterial counter according to the improvement of milk quality and the change of milk quality grade. Therefore, this study was evaluated the conversion mode of the two machines by SPC method. We collected 921 bulk-tank milk samples throughout the concentration range of 1,000~1,000,000 CFU/mL from June 2020 to October 2021. As a result, when compared by the SPC value, there was a slight difference in total bacterial count in BactoScan below 10,000 CFU/mL and above 200,000 CFU/mL and in BactoCount above 100,000 CFU/mL, respectively. Therefore, the conversion factor for BactoScan and the conversion equation for BactoCount were newly adjusted based on SPC value, and then the correlation coefficients (R2) was 0.85 or higher. In addition, the correlation (R2) between BactoScan and BactoCount was 0.91, which means the results were high positive correlation. These results are expected to contribute to improving the accuracy of the automated instruments for determining of total bacterial count in raw milk.

Keywords

Acknowledgement

원유 세균수 검사 시료 제공에 도움을 주신 경북동물위생시험소 본소 관계자분들에게 감사드린다. 본 연구는 2020년 농림축산검역본부의 농림축산검역검사기술개발사업(원유 세균수 검사장비 표준화에 관한 연구, N-1543081-2020-21-02)의 연구비 지원에 의해 수행되었다.

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