Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular characterization of H3N2 influenza A virus isolated from a pig by next generation sequencing in Korea

  • Oh, Yeonsu (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Moon, Sung-Hyun (College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University) ;
  • Ko, Young-Seung (College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University) ;
  • Na, Eun-Jee (College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University) ;
  • Tark, Dong-Seob (Korea Zoonosis Research Institute, Jeonbuk National University) ;
  • Oem, Jae-Ku (College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University) ;
  • Kim, Won-Il (College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University) ;
  • Rim, Chaekwang (RCK Co. Ltd.) ;
  • Cho, Ho-Seong (Korea Zoonosis Research Institute, Jeonbuk National University)
  • Received : 2022.03.29
  • Accepted : 2022.03.30
  • Published : 2022.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Swine influenza (SI) is an important respiratory disease in pigs and epidemic worldwide, which is caused by influenza A virus (IAV) belonging to the family of Orthomyxoviridae. As seen again in the 2009 swine-origin influenza A H1N1 pandemic, pigs are known to be susceptible to swine, avian, and human IAVs, and can serve as a 'mixing vessel' for the generation of novel IAV variants. To this end, the emergence of swine influenza viruses must be kept under close surveillance. Herein, we report the isolation and phylogenetic study of a swine IAV, A/swine/Korea/21810/2021 (sw21810, H3N2 subtype). BLASTN sequence analysis of 8 gene segments of the isolated virus revealed a high degree of nucleotide similarity (94.76 to 100%) to porcine strains circulating in Korea and the United States. Out of 8 genome segments, the HA gene was closely related to that of isolates from cluster I. Additionally, the NA gene of the isolate belonged to a Korean Swine H1N1 origin, and the PB2, PB1, NP and NS genes of the isolate were grouped into that of the Triple reassortant swine H3N2 origin virus. The PA and M genes of the isolate belonged to 2009 Pandemic H1N1 lineage. Human infection with mutants was most common through contact with infected pigs. Our results suggest the need for periodic close monitoring of this novel swine H3N2 influenza virus from a public health perspective.

Keywords

Acknowledgement

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Animal Disease Management Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (321008-1).

References

  1. Cabanski CR, Magrini V, Griffith M, Griffith OL, McGrath S, Zhang J, Walker J, Ly A, Demeter R, Fulton RS, Pong WW, Gutmann DH, Govindan R, Mardis ER, Maher CA. 2014. cDNA hybrid capture improves transcriptome analysis on low-input and archived samples. J Mol Diagn 16(4): 440-451. https://doi.org/10.1016/j.jmoldx.2014.03.004
  2. Graham RM, Doyle CJ, Jennison AV. 2014. Real-time investigation of a Legionella pneumophila outbreak using whole genome sequencing. Epidemiol Infect 142(11): 2347-23451. https://doi.org/10.1017/S0950268814000375
  3. Kim JI, Lee I, Park S, Lee S, Hwang MW, Bae JY, Heo J, Kim D, Jang SI, Kim K, Park MS. 2014. Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012. PLoS One 9(2): e88782. https://doi.org/10.1371/journal.pone.0088782
  4. Kim SH, Kim HJ, Jin YH, Yeoul JJ, Lee KK, Oem JK, Lee MH, Park CK. 2013. Isolation of influenza A(H3N2)v virus from pigs and characterization of its biological properties in pigs and mice. Arch Virol 158(11): 2351-2357. https://doi.org/10.1007/s00705-012-1571-9
  5. Noh JY, Lo VT, Kim YJ, Yoon SW, Jeong DG, Na W, Song D, Kim HK. 2020. Complete Coding Sequence of a Swine Influenza A Variant (H3N2) Virus Isolated in the Republic of Korea in 2017. Microbiol Resour Announc 9(7): e01355-19.
  6. Scholtissek C. 1990. Pigs as the "mixing vessel" for the creation of new pandemic influenza A viruses. Med Princ Pract 2: 65-71. https://doi.org/10.1159/000157337
  7. Tindale LC, Baticados W, Duan J, Coombe M, Jassem A, Tang P, Uyaguari-Diaz M, Moore R, Himsworth C, Hsiao W, Prystajecky N. 2020. Extraction and Detection of Avian Influenza Virus From Wetland Sediment Using Enrichment-Based Targeted Resequencing. Front Vet Sci 7: 301. https://doi.org/10.3389/fvets.2020.00301
  8. Tong S, Zhu X, Li Y, Shi M, Zhang J, Bourgeois M, Yang H, Chen X, Recuenco S, Gomez J, Chen LM, Johnson A, Tao Y, Dreyfus C, Yu W, McBride R, Carney PJ, Gilbert AT, Chang J, Guo Z, Davis CT, Paulson JC, Stevens J, Rupprecht CE, Holmes EC, Wilson IA, Donis RO. 2013. New world bats harbor diverse influenza A viruses. PLoS Pathog 9(10): e1003657. https://doi.org/10.1371/journal.ppat.1003657
  9. Trebbien R, Bragstad K, Larsen LE, Nielsen J, Botner A, Heegaard PM, Fomsgaard A, Viuff B, Hjulsager CK. 2013. Genetic and biological characterisation of an avian-like H1N2 swine influenza virus generated by reassortment of circulating avian-like H1N1 and H3N2 subtypes in Denmark. Virol J 10: 290. https://doi.org/10.1186/1743-422X-10-290
  10. Turner J, O'Neill P, Grant M, Mumford RA, Thwaites R, Studholme DJ. 2017. Genome sequences of 12 isolates of the EU1 lineage of Phytophthora ramorum, a fungus-like pathogen that causes extensive damage and mortality to a wide range of trees and other plants. Genom Data 12: 17-21. https://doi.org/10.1016/j.gdata.2017.02.006
  11. Van Reeth K, Vincent A. 2019. Influenza viruses. Pp. 576-593. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, Zhang J (ed.). Diseases of Swine. John Willey and Sons Inc., Ames, IA.
  12. Zhou B, Donnelly ME, Scholes DT, St George K, Hatta M, Kawaoka Y, Wentworth DE. 2009. Single-reaction genomic amplification accelerates sequencing and vaccine production for classical and Swine origin human influenza A viruses. J Virol 83(19): 10309-10313. https://doi.org/10.1128/JVI.01109-09