Browse > Article
http://dx.doi.org/10.7853/kjvs.2022.45.1.39

Re-evaluation on conversion system of BactoScan and BactoCount for raw milk in South Korea  

Kim, ESeul (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Kim, Jin-Hwan (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Byun, Yeong-Seob (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Park, Dasom (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Kim, Ha-Young (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Yoon, Soon-Seek (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Moon, Jin-San (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Publication Information
Korean Journal of Veterinary Service / v.45, no.1, 2022 , pp. 39-45 More about this Journal
Abstract
The total bacteria count is significantly important factor for hygienic quality in raw milk. BactoScan FMTM and BactoCount IBCTM are the automated instruments for the determination of the total bacterial count in raw milk. They have been used after calibration by standard plate count (SPC) method in South Korea since 2000. It is necessary to re-evaluate for total bacterial counter according to the improvement of milk quality and the change of milk quality grade. Therefore, this study was evaluated the conversion mode of the two machines by SPC method. We collected 921 bulk-tank milk samples throughout the concentration range of 1,000~1,000,000 CFU/mL from June 2020 to October 2021. As a result, when compared by the SPC value, there was a slight difference in total bacterial count in BactoScan below 10,000 CFU/mL and above 200,000 CFU/mL and in BactoCount above 100,000 CFU/mL, respectively. Therefore, the conversion factor for BactoScan and the conversion equation for BactoCount were newly adjusted based on SPC value, and then the correlation coefficients (R2) was 0.85 or higher. In addition, the correlation (R2) between BactoScan and BactoCount was 0.91, which means the results were high positive correlation. These results are expected to contribute to improving the accuracy of the automated instruments for determining of total bacterial count in raw milk.
Keywords
BactoCount; BactoScan; Conversion system; Raw milk; Total bacterial count;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 ISO 21187-1:2003. Milk - Quantitative determination of bacteriological quality - Guidance for establishing a conversion relationship between routine method results and anchor method results and its verification. www.iso.org.
2 Walter S, Thomas B. 2016. Application of a unified European conversion factor for the transformation of Bactoscan FC values. Food Agroscope Science 36: 1-11.
3 농림축산검역본부. 2020. 2020년 원유검사 표준화 교육. pp. 41-48.
4 농림축산검역본부. 2021. 원유검사 표준용액 제조 및 사용지침서. pp. 101-125.
5 Cunningham JD, Connie LS. 1988. Collaborative study of the bactoscan-an automated method for determinations of total bacteria in raw milk. Canadian Institute of Food Science and Technology Journal 21(5): 464-466.   DOI
6 Desmeasures NM, Gueguen M. 1997. Monitoring the microbiology of high quality milk by monthly sampling over 2 years. J Dairy Res 64: 271-280.   DOI
7 Madden RH, Gordon A, Corcionivoschi N. 2017. Determination of the BactoScan conversion Factor for the United Kingdom. Dairy microbiology 70: 17-19.
8 IDF. 1998. Milk payment systems for ex-farm milk. no 3331. Brussels, Belgium.
9 ISO 16140:2003. Microbiology of the food and animal feeding stuffs-Protocol for the validation of alternative methods. www.iso.org.
10 ISO 4833-1:2013. Microbiology of the food chain Horizontal method for the enumeration of microorganisms Part 1: Colony count at 30 degrees C by the pour plate technique. pp. 9. www.iso.org.
11 Suhren G, Walte HG. 2000. First experiences with automatic flow cytometric determination of total bacterial count in raw milk. Bulletin of the International Dairy Federation. 50: 249-275.
12 IDF. 1991. Milk and milk products enumeration of microorganisms colony count technique at 37℃. FILIDF standard no 100B. Brussels, Belgium.
13 농림축산검역본부. 2019. 원유검사 표준화 운영 실무 가이드라인. pp. 43-52.
14 Bolzoni G, Marcolini A, Fontana A, Monaco L, Ferrini AM. 2017. BactoScan FC: conversion system for results at the national level in Italy and reproducibility of total bacterial counting four years after implementation. Euroreference 46-54.
15 Gunasekera TS, Attfield PV, Veal DA. 2000. A flow cytometry method for rapid detection and enumeration of total bacterial bacteria in milk. Appl Environ Microbiol 66: 1228-1232.   DOI
16 IDF. 2004. Milk - Quantitive determination of microbiological quality - Guidance on establishing and verifying a conversion relationship between routine method results and anchor method results. FIL-IDF standard no 196. Brussels, Belgium.
17 REGULATION(EC) No 853/2004 of the European parliament and of the council of 29 April 2004 laying down specific hygiene rules for the hygiene of foodstuffs.
18 ISO 16297/IDF 161. 2013. Milk - Bacterial count- Protocol for the evaluation of alternative method. www.iso.org.
19 ISO 4833:2003. Microbiology of the food and animal feeding stuffs-Horizontal method for the enumeration of microorganisms-Colony count technique at 30 degrees C. www.iso.org.
20 Gunasekera TS, Veal DA, Attfield PV. 2003. Potential for broad applications of flow cytometry and fluorescence technique in microbiological and somatic cell analysis of milk. Int J Food Microbiol 85(3): 269-279.   DOI
21 Kang HJ, Kim JH, Byun YS, Lee H, Lee HY, Kim J, Hong S, Kim HY, Yoon SS, Moon JS. 2020. Development and evaluation of standard samples for quality control of automated total bacterial counter in raw milk. Korean J Vet Serv 43(3): 147-154.   DOI