Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antibacterial Effect of Chitosan-Modified Fe3O4 Nanozymes on Acinetobacter baumannii

  • Wang, Wenjun (The First Affiliated Hospital of Guangzhou Medical University) ;
  • Wu, ziman (The First Affiliated Hospital of Guangzhou Medical University) ;
  • Shi, peiru (Guangzhou Medical University (KingMed school of Laboratory Medicine)) ;
  • Wu, pinyun (Guangzhou Medical University (KingMed school of Laboratory Medicine)) ;
  • Qin, peng (Guangzhou Medical University (KingMed school of Laboratory Medicine)) ;
  • Yu, lin (The First Affiliated Hospital of Guangzhou Medical University)
  • Received : 2021.07.27
  • Accepted : 2021.10.17
  • Published : 2022.02.28
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Abstract

The aim of this study was to determine whether the antibacterial activity of chitosan-modified Fe3O4 (CS@Fe3O4) nanomaterials against Acinetobacter baumannii (A. baumannii) is mediated through changes in biofilm formation and reactive oxygen species (ROS) production. For this purpose, the broth dilution method was used to examine the effect of CS@Fe3O4 nanoparticles on bacterial growth. The effects of CS@Fe3O4 nanoparticles on biofilm formation were measured using a semi-quantitative crystal violet staining assay. In addition, a bacterial ROS detection kit was used to detect the production of ROS in bacteria. The results showed that CS@Fe3O4 nanoparticles had a significant inhibitory effect on the colony growth and biofilm formation of drug-resistant A. baumannii (p < 0.05). The ROS stress assay revealed significantly higher ROS levels in A. baumannii subjected to CS@Fe3O4 nanoparticle treatment than the control group (p < 0.05). Thus, we demonstrated for the first time that CS@Fe3O4 nanoparticles had an inhibitory effect on A. baumannii in vitro, and that the antibacterial effect of CS@Fe3O4 nanoparticles on drug-resistant A. baumannii was more significant than on drug-sensitive bacteria. Our findings suggest that the antibacterial mechanism of CS@Fe3O4 nanoparticles is mediated through inhibition of biofilm formation in drug-resistant bacteria, as well as stimulation of A. baumannii to produce ROS. In summary, our data indicate that CS@Fe3O4 nanoparticles could be used to treat infections caused by drug-resistant A. baumannii.

Keywords

Acknowledgement

This work was funded by the Science and Technology Innovation Project of Guangzhou Medical University (2019A075 & 2020A063), Zhongnanshan Medical Foundation of Guangdong Province, and the Penghua Care Fund to the Medical Pioneers against Covid-19 of Shenzhen Social Commonweal Foundation.

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