Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Heat-Killed Lactiplantibacillus plantarum LRCC5314 Mitigates the Effects of Stress-Related Type 2 Diabetes in Mice via Gut Microbiome Modulation

  • Nam, YoHan (Department of Microbiology, College of Medicine, Chung-Ang University) ;
  • Yoon, Seokmin (Department of Microbiology, College of Medicine, Chung-Ang University) ;
  • Baek, Jihye (Department of Microbiology, College of Medicine, Chung-Ang University) ;
  • Kim, Jong-Hwa (Department of Microbiology, College of Medicine, Chung-Ang University) ;
  • Park, Miri (Lotte R&D Center) ;
  • Hwang, KwangWoo (College of Pharmacy, 2 Chung‐Ang University) ;
  • Kim, Wonyong (Department of Microbiology, College of Medicine, Chung-Ang University)
  • Received : 2021.11.04
  • Accepted : 2021.12.15
  • Published : 2022.03.28
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Abstract

The incidence of stress-related type 2 diabetes (stress-T2D), which is aggravated by physiological stress, is increasing annually. The effects of Lactobacillus, a key component of probiotics, have been widely studied in diabetes; however, studies on the effects of postbiotics are still limited. Here, we aimed to examine the mechanism through which heat-killed Lactiplantibacillus plantarum LRCC5314 (HK-LRCC5314) alleviates stress-T2D in a cold-induced stress-T2D C57BL/6 mouse model. HK-LRCC5314 markedly decreased body weight gain, adipose tissue (neck, subcutaneous, and epididymal) weight, and fasting glucose levels. In the adipose tissue, mRNA expression levels of stress-T2D associated factors (NPY, Y2R, GLUT4, adiponectin, and leptin) and pro-inflammatory factors (TNF-α, IL-6, and CCL-2) were also altered. Furthermore, HK-LRCC5314 increased the abundance of Barnesiella, Alistipes, and butyrate-producing bacteria, including Akkermansia, in feces and decreased the abundance of Ruminococcus, Dorea, and Clostridium. Thus, these findings suggest that HK-LRCC5314 exerts protective effects against stress-T2D via gut microbiome modulation, suggesting its potential as a supplement for managing stress-T2D.

Keywords

Acknowledgement

This work was supported by the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea [as part of the (multi-ministerial) Genome Technology-to-Business Translation Program; No. 918004-4] and the Chung-Ang University Research Scholarship Grant in 2021.

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