한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제35권1호
  • /
  • Pages.1-10
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  • 2022
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
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DOI QR Code

산백국(山白菊) 열수추출물이 RAW 264.7 대식세포에 미치는 항염증 효과

Anti-inflammatory Effects of Aster glehni Water Extracts in LPS-stimulated RAW 264.7 Macrophages

  • 고호건 (상지대학교 한의과대학 안이비인후피부과학교실) ;
  • 이규영 (상지대학교 한의과대학 안이비인후피부과학교실) ;
  • 홍철희 (상지대학교 한의과대학 안이비인후피부과학교실)
  • Ko, Ho-Geon (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University) ;
  • Lee, Kyou-Young (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University) ;
  • Hong, Chul-Hee (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
  • 투고 : 2022.01.14
  • 심사 : 2022.02.11
  • 발행 : 2022.02.25
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초록

Objectives : This study was conducted to confirm the anti-inflammatory effects of Aster glehni Water extracts. Methods : In this study, MTT assay was performed to detect cell viability. To evaluate the anti-inflammatory effects of Aster glehni Water extracts, we examined NO production in LPS-induced macrophages. Expressions of iNOS, COX-2, ERK, p38, JNK were also investigated by using western blot assay. Results : Aster glehni Water extracts have no cytotoxicity at 15.625-1,000㎍/㎖ in RAW 264.7 cells. Aster glehni Extracts inhibited the NO production in a dose-dependent manner in RAW 264.7 cells treated with LPS. Pretreated 250, 500, 1,000㎍/㎖ of Aster glehni water extracts had significantly suppressed expression levels of iNOS, COX-2, p-ERK, p-p38, p-JNK. Conclusions : These results suggest that Aster glehni Water extracts have anti-inflammatory effects and can be used for various inflammatory skin diseases.

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참고문헌

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