Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Analysis of Behavioral Changes in Angelfish (Pterophyllum scalare) Infected with Bacterial Pathogens using Video Tracking

Video tracking을 이용한 병원성 세균에 감염된 angelfish (Pterophyllum scalare)의 행동 변화 분석

  • Yoon-Jae, Kim (Department of aquatic life medicine, Pukyong National University) ;
  • Young-Ung, Heo (Department of aquatic life medicine, Pukyong National University) ;
  • Ju-Sung, Kim (Department of aquatic life medicine, Pukyong National University) ;
  • Min-Kyo, Kim (Department of aquatic life medicine, Pukyong National University) ;
  • Do-Hyung, Kim (Department of aquatic life medicine, Pukyong National University)
  • 김윤재 (부경대학교 수산생명의학과) ;
  • 허영웅 (부경대학교 수산생명의학과) ;
  • 김주성 (부경대학교 수산생명의학과) ;
  • 김민교 (부경대학교 수산생명의학과) ;
  • 김도형 (부경대학교 수산생명의학과)
  • Received : 2022.10.11
  • Accepted : 2022.12.01
  • Published : 2022.12.31
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Abstract

In recent years, there have been many studies investigating changes in animal behavior using video tracking technology to track motion. However, there have been very few studies and results on changes in the behavior of fish infected with a pathogen. Therefore, the present study attempted to analyze the behavior of angelfish (Pterophyllum scalare) infected with bacterial pathogens using video tracking. Two cameras were placed in front of the water tank to obtain behavior data, and tracking was performed for three days until the day of death. Data such as average speed, changes in speed, the locations of the fish in the tank, and fractal dimension were statistically analyzed based on the fish speed and location in the tank of the fish. For bacterial infection, an individual angelfish was intraperitoneally injected with approximately 106 CFU ml-1 of Aeromonas hydrophila or Edwardsiella piscicida. The experiment was carried out five times for each group. Fish infected with the bacterial pathogens showed a tendency to increase in speed and to spend more time in the upper part of the tank one or two days before death. On the day the fish died, the average speed, changes in speed, and the fractal dimension value were significantly lower than the corresponding values in the control group, and the fish also remained in the lower part of the tank. Our results indicated that behavioral changes in fish could be successfully detected earlier than death using video tracking technology, and that this method presents potential for disease monitoring in aquaculture.

Keywords

Acknowledgement

이 논문은 2022학년도 부경대학교 국립대학육성사업 지원비(PhiNX 보호학문 차세대육성)에 의하여 연구되었음.

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